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Evolution of hepatitis C virus variants following blood transfusion from one infected donor to several recipients: a long-term follow-up

Love, Arthur; Molnegren, Vilma; Månsson, Ann-Sofie LU ; Smáradóttir, Agnes; Thorsteinsson, Sigurdur B. and Widell, Anders LU (2004) In Journal of General Virology 85(2). p.441-450
Abstract
Variants of hepatitis C virus (HCV) from a single infected blood donor and 13 viraemic recipients who were traced were examined by sequencing and cloning to determine the extent of virus diversity in hypervariable region 1. Serum-derived viral isolates were studied from the donor when his HCV infection was discovered in 1993, in his recipients that year (0·3–5 years post-transfusion) and 5 years later in the donor and six viraemic recipients who were still alive. Viral variants of broad diversity were readily demonstrated in the baseline samples of the donor (nucleotide p-distance 0·130), but significantly less (P<0·00003) diversity was observed in the recipients' first samples (p-distances within recipients 0·003–0·062). In the first... (More)
Variants of hepatitis C virus (HCV) from a single infected blood donor and 13 viraemic recipients who were traced were examined by sequencing and cloning to determine the extent of virus diversity in hypervariable region 1. Serum-derived viral isolates were studied from the donor when his HCV infection was discovered in 1993, in his recipients that year (0·3–5 years post-transfusion) and 5 years later in the donor and six viraemic recipients who were still alive. Viral variants of broad diversity were readily demonstrated in the baseline samples of the donor (nucleotide p-distance 0·130), but significantly less (P<0·00003) diversity was observed in the recipients' first samples (p-distances within recipients 0·003–0·062). In the first blood samples of the recipients, many of the viral variants identified were closely related to a strain variant from the donor. In follow-up samples drawn 5 years later from the donor and six recipients, the p-distance among donor clones had increased (0·172, P<0·0005) compared with the recipients, who displayed significantly narrower quasispecies (0·011–0·086). A common finding was that recipients of blood components processed from the same donation differed substantially in persisting HCV infectious sequence. Markedly few changes leading to changes of amino acids had occurred during follow-up in four of six recipients. These results question the significance of the development of viral variants as a necessary phenomenon in the evolution of HCV and pathogenesis of the disease. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of General Virology
volume
85
issue
2
pages
441 - 450
publisher
Society for General Microbiology
external identifiers
  • wos:000189152500018
  • scopus:1342267790
ISSN
1465-2099
DOI
10.1099/vir.0.19439-0
language
English
LU publication?
yes
id
3155d9c2-04af-47dd-94b8-34392646ba86 (old id 141973)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14769902&query_hl=134
date added to LUP
2007-07-25 16:16:15
date last changed
2017-03-12 04:12:05
@article{3155d9c2-04af-47dd-94b8-34392646ba86,
  abstract     = {Variants of hepatitis C virus (HCV) from a single infected blood donor and 13 viraemic recipients who were traced were examined by sequencing and cloning to determine the extent of virus diversity in hypervariable region 1. Serum-derived viral isolates were studied from the donor when his HCV infection was discovered in 1993, in his recipients that year (0·3–5 years post-transfusion) and 5 years later in the donor and six viraemic recipients who were still alive. Viral variants of broad diversity were readily demonstrated in the baseline samples of the donor (nucleotide p-distance 0·130), but significantly less (P&lt;0·00003) diversity was observed in the recipients' first samples (p-distances within recipients 0·003–0·062). In the first blood samples of the recipients, many of the viral variants identified were closely related to a strain variant from the donor. In follow-up samples drawn 5 years later from the donor and six recipients, the p-distance among donor clones had increased (0·172, P&lt;0·0005) compared with the recipients, who displayed significantly narrower quasispecies (0·011–0·086). A common finding was that recipients of blood components processed from the same donation differed substantially in persisting HCV infectious sequence. Markedly few changes leading to changes of amino acids had occurred during follow-up in four of six recipients. These results question the significance of the development of viral variants as a necessary phenomenon in the evolution of HCV and pathogenesis of the disease.},
  author       = {Love, Arthur and Molnegren, Vilma and Månsson, Ann-Sofie and Smáradóttir, Agnes and Thorsteinsson, Sigurdur B. and Widell, Anders},
  issn         = {1465-2099},
  language     = {eng},
  number       = {2},
  pages        = {441--450},
  publisher    = {Society for General Microbiology},
  series       = {Journal of General Virology},
  title        = {Evolution of hepatitis C virus variants following blood transfusion from one infected donor to several recipients: a long-term follow-up},
  url          = {http://dx.doi.org/10.1099/vir.0.19439-0},
  volume       = {85},
  year         = {2004},
}