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Effects in vitro and in vivo by apomorphine in the rat corpus cavernosum.

Matsumoto, Kenshi LU ; Yoshida, Masaki; Andersson, Karl-Erik LU and Hedlund, Petter LU (2005) In British Journal of Pharmacology 146(2). p.259-267
Abstract
1 The study was performed to clarify if apomorphine at the level of the rat corpus cavernosum can produce erectile responses or interfere with nerve-induced penile erection. 2 Apomorphine ( 10(-9) - 10(-4) M) exhibited a 10-fold higher potency to relax phenylephrine ( Phe)-than endothelin-1 ( ET-1)-induced contractions. Relaxant effects of apomorphine in Phe-activated corpus cavernosum did not change tissue levels of cyclic nucleotides,and were unaffected by inhibition of the synthesis of nitric oxide, or by inhibition of the soluble guanylate cyclase. 3 Relaxations by apomorphine of ET-1-contracted rat corpus cavernosum were not influenced by alpha(2)-adrenoceptor blockade ( yohimbine, 10(-7) M), or by the dopamine D1-like receptor... (More)
1 The study was performed to clarify if apomorphine at the level of the rat corpus cavernosum can produce erectile responses or interfere with nerve-induced penile erection. 2 Apomorphine ( 10(-9) - 10(-4) M) exhibited a 10-fold higher potency to relax phenylephrine ( Phe)-than endothelin-1 ( ET-1)-induced contractions. Relaxant effects of apomorphine in Phe-activated corpus cavernosum did not change tissue levels of cyclic nucleotides,and were unaffected by inhibition of the synthesis of nitric oxide, or by inhibition of the soluble guanylate cyclase. 3 Relaxations by apomorphine of ET-1-contracted rat corpus cavernosum were not influenced by alpha(2)-adrenoceptor blockade ( yohimbine, 10(-7) M), or by the dopamine D1-like receptor antagonist SCH 23390 ( 10(-6) M). Clozapine ( 10(-6) M), a proposed dopamine D2-like receptor antagonist, partly reduced apomorphine-induced relaxations, and significantly altered the - log IC50 value for apomorphine. 4 Nerve-induced contractions of the rat corpus cavernosum were attenuated by apomorphine in a concentration-dependent and biphasic manner. Yohimbine ( 10(-7) M) abolished the biphasic concentration - response pattern. SCH 23390 ( 10(-6) M) attenuated the inhibitory effects of apomorphine on contractions, and significantly altered the - log IC50 value for the compound. 5 In anesthetized rats ( 50 mg kg(-1) pentobarbital sodium, 10 mg kg(-1) ketamine), intracavernous apomorphine ( 100, 300, or 1000 nmol) did not have effects on basal cavernous pressure under resting conditions, and did not affect filling or emptying rates, or peak pressures of the rat corpus cavernosum during submaximal activation of the cavernous nerve. In awake rats, apomorphine produced a maximal number of erections at 300 nmol kg(-1). 6 In the rat isolated corpus cavernosum, pre- and postjunctional effects of apomorphine appear to involve dopamine D1- and D2-like receptors, as well as alpha-adrenoceptors. At relevant systemic doses of apomorphine, peripheral effects of the compound are unlikely to contribute to its proerectile effects in rats. (Less)
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organization
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Contribution to journal
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published
subject
keywords
alpha-adrenoceptors, apomorphine, dopamine receptors, rat, corpus cavernosum, relaxation
in
British Journal of Pharmacology
volume
146
issue
2
pages
259 - 267
publisher
The British Pharmacological Society
external identifiers
  • wos:000232317100013
  • pmid:16025145
  • scopus:30544445518
ISSN
1476-5381
DOI
10.1038/sj.bjp.0706317
language
English
LU publication?
yes
id
44a5a19e-d40d-4e71-abde-e634ac2a6fb0 (old id 142091)
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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16025145&dopt=Abstract
date added to LUP
2007-07-05 13:49:01
date last changed
2017-10-22 04:29:28
@article{44a5a19e-d40d-4e71-abde-e634ac2a6fb0,
  abstract     = {1 The study was performed to clarify if apomorphine at the level of the rat corpus cavernosum can produce erectile responses or interfere with nerve-induced penile erection. 2 Apomorphine ( 10(-9) - 10(-4) M) exhibited a 10-fold higher potency to relax phenylephrine ( Phe)-than endothelin-1 ( ET-1)-induced contractions. Relaxant effects of apomorphine in Phe-activated corpus cavernosum did not change tissue levels of cyclic nucleotides,and were unaffected by inhibition of the synthesis of nitric oxide, or by inhibition of the soluble guanylate cyclase. 3 Relaxations by apomorphine of ET-1-contracted rat corpus cavernosum were not influenced by alpha(2)-adrenoceptor blockade ( yohimbine, 10(-7) M), or by the dopamine D1-like receptor antagonist SCH 23390 ( 10(-6) M). Clozapine ( 10(-6) M), a proposed dopamine D2-like receptor antagonist, partly reduced apomorphine-induced relaxations, and significantly altered the - log IC50 value for apomorphine. 4 Nerve-induced contractions of the rat corpus cavernosum were attenuated by apomorphine in a concentration-dependent and biphasic manner. Yohimbine ( 10(-7) M) abolished the biphasic concentration - response pattern. SCH 23390 ( 10(-6) M) attenuated the inhibitory effects of apomorphine on contractions, and significantly altered the - log IC50 value for the compound. 5 In anesthetized rats ( 50 mg kg(-1) pentobarbital sodium, 10 mg kg(-1) ketamine), intracavernous apomorphine ( 100, 300, or 1000 nmol) did not have effects on basal cavernous pressure under resting conditions, and did not affect filling or emptying rates, or peak pressures of the rat corpus cavernosum during submaximal activation of the cavernous nerve. In awake rats, apomorphine produced a maximal number of erections at 300 nmol kg(-1). 6 In the rat isolated corpus cavernosum, pre- and postjunctional effects of apomorphine appear to involve dopamine D1- and D2-like receptors, as well as alpha-adrenoceptors. At relevant systemic doses of apomorphine, peripheral effects of the compound are unlikely to contribute to its proerectile effects in rats.},
  author       = {Matsumoto, Kenshi and Yoshida, Masaki and Andersson, Karl-Erik and Hedlund, Petter},
  issn         = {1476-5381},
  keyword      = {alpha-adrenoceptors,apomorphine,dopamine receptors,rat,corpus cavernosum,relaxation},
  language     = {eng},
  number       = {2},
  pages        = {259--267},
  publisher    = {The British Pharmacological Society},
  series       = {British Journal of Pharmacology},
  title        = {Effects in vitro and in vivo by apomorphine in the rat corpus cavernosum.},
  url          = {http://dx.doi.org/10.1038/sj.bjp.0706317},
  volume       = {146},
  year         = {2005},
}