Advanced

Optical Lectin Based Biosensor as Tool for Bacteria Identification

Masarova, Jana LU ; Dey, Estera LU and Danielsson, Bengt LU (2004) In Polish Journal of Microbiology2004-01-01+01:00 53(Supplement). p.23-27
Abstract
Biosensor techniques are based on biospecific interaction between the biological parts of biosensor with the analyte. In biosensor construction, antibodies are usually used for the detection of analytes such as microorganism, because of very strong and highly specific interaction. The disadvantages of this assay are a long time needed for antibody isolation and purification as well as difficult regeneration of biosensor chip. The use of lectins instead of antibodies could solve these problems because a several hundred lectins are commercially available and their stability in standard buffers is better compared to monoclonal antibodies. While antibody can only be used to detect that antigen it was designed for, lectin as low affinity... (More)
Biosensor techniques are based on biospecific interaction between the biological parts of biosensor with the analyte. In biosensor construction, antibodies are usually used for the detection of analytes such as microorganism, because of very strong and highly specific interaction. The disadvantages of this assay are a long time needed for antibody isolation and purification as well as difficult regeneration of biosensor chip. The use of lectins instead of antibodies could solve these problems because a several hundred lectins are commercially available and their stability in standard buffers is better compared to monoclonal antibodies. While antibody can only be used to detect that antigen it was designed for, lectin as low affinity molecule may bind several different pathogens. Using the discriminative effect of an artificial neural network the application of a lectin array will compensate for the lower specificity.

Microbial surfaces bear many of the sugar residues capable of interacting with lectins. The ability of lectins to react with microbial glycoconjugates means that it is possible to employ them as probes and sorbents for whole cells, mutants and numerous cellular constituents and metabolites, and it makes them useful tools for identification or typing of bacteria. Lectins are attractive reagents for the clinical diagnostic laboratory because of their diverse specificity, commercial availability, a wide range of molecular weights, and their stability in standard buffers. The construction of lectin biosensor could be an advantage method for detection of pathogenic bacteria. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Polish Journal of Microbiology2004-01-01+01:00
volume
53
issue
Supplement
pages
23 - 27
publisher
Polskie Towarzystwo Mikrobiologów
external identifiers
  • scopus:11244272770
ISSN
1733-1331
language
English
LU publication?
yes
id
13ae2cd1-2f64-47ea-86b4-90c1b53a50cb (old id 142517)
alternative location
http://www.amp.microbiology.pl/full/micros_2004.pdf
date added to LUP
2007-07-16 10:44:37
date last changed
2017-01-01 06:43:43
@article{13ae2cd1-2f64-47ea-86b4-90c1b53a50cb,
  abstract     = {Biosensor techniques are based on biospecific interaction between the biological parts of biosensor with the analyte. In biosensor construction, antibodies are usually used for the detection of analytes such as microorganism, because of very strong and highly specific interaction. The disadvantages of this assay are a long time needed for antibody isolation and purification as well as difficult regeneration of biosensor chip. The use of lectins instead of antibodies could solve these problems because a several hundred lectins are commercially available and their stability in standard buffers is better compared to monoclonal antibodies. While antibody can only be used to detect that antigen it was designed for, lectin as low affinity molecule may bind several different pathogens. Using the discriminative effect of an artificial neural network the application of a lectin array will compensate for the lower specificity.<br/><br>
Microbial surfaces bear many of the sugar residues capable of interacting with lectins. The ability of lectins to react with microbial glycoconjugates means that it is possible to employ them as probes and sorbents for whole cells, mutants and numerous cellular constituents and metabolites, and it makes them useful tools for identification or typing of bacteria. Lectins are attractive reagents for the clinical diagnostic laboratory because of their diverse specificity, commercial availability, a wide range of molecular weights, and their stability in standard buffers. The construction of lectin biosensor could be an advantage method for detection of pathogenic bacteria.},
  author       = {Masarova, Jana and Dey, Estera and Danielsson, Bengt},
  issn         = {1733-1331},
  language     = {eng},
  number       = {Supplement},
  pages        = {23--27},
  publisher    = {Polskie Towarzystwo Mikrobiologów},
  series       = {Polish Journal of Microbiology2004-01-01+01:00},
  title        = {Optical Lectin Based Biosensor as Tool for Bacteria Identification},
  volume       = {53},
  year         = {2004},
}