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Acute retinal ischemia caused by controlled low ocular perfusion pressure in a porcine model. Electrophysiological and histological characterisation

Kyhn, Maria Voss; Warfvinge, Karin LU ; Scherfig, Erik; Kiilgaard, Jens F.; Prause, Jan Ulrik; Klassen, Henry; Young, Michael and la Cour, Morten (2009) In Experimental Eye Research 88(6). p.1100-1106
Abstract
The purpose of this study was to establish, and characterize a porcine model of acute, controlled retinal ischemia. The controlled retinal ischemia was produced by clamping the ocular perfusion pressure (OPP) in the left eye to 5 mm Hg for 2 h. The OPP was defined as mean arterial blood pressure (MAP) minus the intraocular pressure (IOP). It was clamped to 0-30 mm Hg by continuous monitoring of MAP and adjustment of the IOP, which was controlled by cannulation of the anterior chamber. Inner retinal function was assessed by induced multifocal electroretinography (mfERG) with comparisons of the amplitudes obtained in the experimental, left eye, and the control, right eye. Quantitative histology was performed to measure the survival of... (More)
The purpose of this study was to establish, and characterize a porcine model of acute, controlled retinal ischemia. The controlled retinal ischemia was produced by clamping the ocular perfusion pressure (OPP) in the left eye to 5 mm Hg for 2 h. The OPP was defined as mean arterial blood pressure (MAP) minus the intraocular pressure (IOP). It was clamped to 0-30 mm Hg by continuous monitoring of MAP and adjustment of the IOP, which was controlled by cannulation of the anterior chamber. Inner retinal function was assessed by induced multifocal electroretinography (mfERG) with comparisons of the amplitudes obtained in the experimental, left eye, and the control, right eye. Quantitative histology was performed to measure the survival of ganglion cells, amacrine cells and horizontal cells 2-6 weeks after the ischemic insult. An OPP of 5 mm Hg for 2 h induced significant reductions in the amplitudes of iN1 to 20% (CI: 13-30%), and iPr2 to 14% (95% CI: 8-22%) of their baseline values. No signs of recovery were found within the 6-week observation period. Quantitative histology revealed a highly significant reduction in the number of ganglion cells, amacrine cells and horizontal cells after the ischemic insult. This model seems to be suitable for investigations of therapeutic initiatives in diseases involving acute retinal ischemia. (C) 2009 Elsevier Ltd. All rights reserved. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
perfusion pressure, low, retinal ischemia, induced components, pig, multifocal ERG, acute glaucoma, anaesthesia
in
Experimental Eye Research
volume
88
issue
6
pages
1100 - 1106
publisher
Elsevier
external identifiers
  • wos:000266312600012
  • scopus:67349188922
ISSN
0014-4835
DOI
10.1016/j.exer.2009.01.016
language
English
LU publication?
yes
id
bb29e11b-b826-4fe6-bd33-7f8fb10d9731 (old id 1425242)
date added to LUP
2009-07-02 16:24:48
date last changed
2017-01-01 04:56:47
@article{bb29e11b-b826-4fe6-bd33-7f8fb10d9731,
  abstract     = {The purpose of this study was to establish, and characterize a porcine model of acute, controlled retinal ischemia. The controlled retinal ischemia was produced by clamping the ocular perfusion pressure (OPP) in the left eye to 5 mm Hg for 2 h. The OPP was defined as mean arterial blood pressure (MAP) minus the intraocular pressure (IOP). It was clamped to 0-30 mm Hg by continuous monitoring of MAP and adjustment of the IOP, which was controlled by cannulation of the anterior chamber. Inner retinal function was assessed by induced multifocal electroretinography (mfERG) with comparisons of the amplitudes obtained in the experimental, left eye, and the control, right eye. Quantitative histology was performed to measure the survival of ganglion cells, amacrine cells and horizontal cells 2-6 weeks after the ischemic insult. An OPP of 5 mm Hg for 2 h induced significant reductions in the amplitudes of iN1 to 20% (CI: 13-30%), and iPr2 to 14% (95% CI: 8-22%) of their baseline values. No signs of recovery were found within the 6-week observation period. Quantitative histology revealed a highly significant reduction in the number of ganglion cells, amacrine cells and horizontal cells after the ischemic insult. This model seems to be suitable for investigations of therapeutic initiatives in diseases involving acute retinal ischemia. (C) 2009 Elsevier Ltd. All rights reserved.},
  author       = {Kyhn, Maria Voss and Warfvinge, Karin and Scherfig, Erik and Kiilgaard, Jens F. and Prause, Jan Ulrik and Klassen, Henry and Young, Michael and la Cour, Morten},
  issn         = {0014-4835},
  keyword      = {perfusion pressure,low,retinal ischemia,induced components,pig,multifocal ERG,acute glaucoma,anaesthesia},
  language     = {eng},
  number       = {6},
  pages        = {1100--1106},
  publisher    = {Elsevier},
  series       = {Experimental Eye Research},
  title        = {Acute retinal ischemia caused by controlled low ocular perfusion pressure in a porcine model. Electrophysiological and histological characterisation},
  url          = {http://dx.doi.org/10.1016/j.exer.2009.01.016},
  volume       = {88},
  year         = {2009},
}