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Femtosecond Carotenoid to Retinal Energy Transfer in Xanthorhodopsin

Polivka, Tomas; Balashov, Sergei P.; Chabera, Pavel; Imasheva, Eleonora S.; Yartsev, Arkady LU ; Sundström, Villy LU and Lanyi, Janos K. (2009) In Biophysical Journal 96(6). p.2268-2277
Abstract
Xanthorhodopsin of the extremely halophilic bacterium Salinibacter ruber represents a novel antenna system. It consists of a carbonyl carotenoid, salinixanthin, bound to a retinal protein that serves as a light-driven transmembrane proton pump similar to bacteriorhodopsin of archaea. Here we apply the femtosecond transient absorption technique to reveal the excited-state dynamics of salinixanthin both in solution and in xanthorhodopsin. The results not only disclose extremely fast energy transfer rates and pathways, they also reveal effects of the binding site on the excited-state properties of the carotenoid. We compared the excited-state dynamics of salinixanthin in xanthorhodopsin and in NaBH4-treated xanthorhodopsin. The NaBH4... (More)
Xanthorhodopsin of the extremely halophilic bacterium Salinibacter ruber represents a novel antenna system. It consists of a carbonyl carotenoid, salinixanthin, bound to a retinal protein that serves as a light-driven transmembrane proton pump similar to bacteriorhodopsin of archaea. Here we apply the femtosecond transient absorption technique to reveal the excited-state dynamics of salinixanthin both in solution and in xanthorhodopsin. The results not only disclose extremely fast energy transfer rates and pathways, they also reveal effects of the binding site on the excited-state properties of the carotenoid. We compared the excited-state dynamics of salinixanthin in xanthorhodopsin and in NaBH4-treated xanthorhodopsin. The NaBH4 treatment prevents energy transfer without perturbing the carotenoid binding site, and allows observation of changes in salinixanthin excited-state dynamics related to specific binding. The S-1 lifetimes of salinixanthin in untreated and NaBH4-treated xanthorhodopsin were identical (3 ps), confirming the absence of the S-1-mediated energy transfer. The kinetics of salinixanthin S-2 decay probed in the near-infrared region demonstrated a change of the S-2 lifetime from 66 fs in untreated xanthorhodopsin to 110 fs in the NaBH4-treated protein. This corresponds to a salinixanthin-retinal energy transfer time of 165 fs and an efficiency of 40%. In addition, binding of salinixanthin to xanthorhodopsin increases the population of the S* state that decays in 6 ps predominantly to the ground state, but a small fraction (<10%) of the S* state generates a triplet state. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biophysical Journal
volume
96
issue
6
pages
2268 - 2277
publisher
Cell Press
external identifiers
  • wos:000266376700023
  • scopus:66149084418
ISSN
1542-0086
DOI
10.1016/j.bpj.2009.01.004
language
English
LU publication?
yes
id
039ca48e-7ba3-47dc-a929-37d7befba644 (old id 1425407)
date added to LUP
2009-07-02 12:11:24
date last changed
2017-11-19 03:30:48
@article{039ca48e-7ba3-47dc-a929-37d7befba644,
  abstract     = {Xanthorhodopsin of the extremely halophilic bacterium Salinibacter ruber represents a novel antenna system. It consists of a carbonyl carotenoid, salinixanthin, bound to a retinal protein that serves as a light-driven transmembrane proton pump similar to bacteriorhodopsin of archaea. Here we apply the femtosecond transient absorption technique to reveal the excited-state dynamics of salinixanthin both in solution and in xanthorhodopsin. The results not only disclose extremely fast energy transfer rates and pathways, they also reveal effects of the binding site on the excited-state properties of the carotenoid. We compared the excited-state dynamics of salinixanthin in xanthorhodopsin and in NaBH4-treated xanthorhodopsin. The NaBH4 treatment prevents energy transfer without perturbing the carotenoid binding site, and allows observation of changes in salinixanthin excited-state dynamics related to specific binding. The S-1 lifetimes of salinixanthin in untreated and NaBH4-treated xanthorhodopsin were identical (3 ps), confirming the absence of the S-1-mediated energy transfer. The kinetics of salinixanthin S-2 decay probed in the near-infrared region demonstrated a change of the S-2 lifetime from 66 fs in untreated xanthorhodopsin to 110 fs in the NaBH4-treated protein. This corresponds to a salinixanthin-retinal energy transfer time of 165 fs and an efficiency of 40%. In addition, binding of salinixanthin to xanthorhodopsin increases the population of the S* state that decays in 6 ps predominantly to the ground state, but a small fraction (&lt;10%) of the S* state generates a triplet state.},
  author       = {Polivka, Tomas and Balashov, Sergei P. and Chabera, Pavel and Imasheva, Eleonora S. and Yartsev, Arkady and Sundström, Villy and Lanyi, Janos K.},
  issn         = {1542-0086},
  language     = {eng},
  number       = {6},
  pages        = {2268--2277},
  publisher    = {Cell Press},
  series       = {Biophysical Journal},
  title        = {Femtosecond Carotenoid to Retinal Energy Transfer in Xanthorhodopsin},
  url          = {http://dx.doi.org/10.1016/j.bpj.2009.01.004},
  volume       = {96},
  year         = {2009},
}