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Application of supercritical carbon dioxide(scCO(2)) for the extraction of glycolipids from Lactobacillus plantarum B-01

Rakhuba, Dzianis; Novik, Galina LU and Dey, Estera LU (2009) In Journal of Supercritical Fluids 49(1). p.45-51
Abstract
Process parameters for the extraction of glycolipids by scCO(2) from Lactobacillus plantarum B-01 were optimized by carrying out a two-level fractional design experiment. The highest glycolipids extract yield was obtained at 250 bar, 40 degrees C, CO2 flow rate, 10 g/min and hydrometanolic co-solvent, 9.3% (v/v). In the lipid extracts the carbohydrate moiety of glycolipids and in phospholipids phosphorus moiety were quantified and compared to the reference material isolated by classical organic solvent extraction. By TLC four groups of glycolipids were identified. A two step scCO(2) extraction process was established, whereby the second step yielded seven times more of glycolipids and four times more of phospholipids than in the extracts... (More)
Process parameters for the extraction of glycolipids by scCO(2) from Lactobacillus plantarum B-01 were optimized by carrying out a two-level fractional design experiment. The highest glycolipids extract yield was obtained at 250 bar, 40 degrees C, CO2 flow rate, 10 g/min and hydrometanolic co-solvent, 9.3% (v/v). In the lipid extracts the carbohydrate moiety of glycolipids and in phospholipids phosphorus moiety were quantified and compared to the reference material isolated by classical organic solvent extraction. By TLC four groups of glycolipids were identified. A two step scCO(2) extraction process was established, whereby the second step yielded seven times more of glycolipids and four times more of phospholipids than in the extracts obtained with organic solvents. Prolonged scCO(2) extraction to 9 h resulted in 620 mu g of glycolipids and 875 mu g of phospholipids in a mg of extract in comparison to (1.5 h) 55 mu g and 15 mu g of glycolipids and phospholipids, respectively. (C) 2008 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Glycolipids, plantarum, Lactobacillus, Supercritical carbon dioxide, Lactic acid bacteria, Phospholipids, Neutral lipids
in
Journal of Supercritical Fluids
volume
49
issue
1
pages
45 - 51
publisher
Elsevier
external identifiers
  • wos:000266178500007
  • scopus:64249142070
ISSN
0896-8446
DOI
10.1016/j.supflu.2008.11.016
language
English
LU publication?
yes
id
b187eb33-c6c8-4cf6-828a-f6c6022e5287 (old id 1425616)
date added to LUP
2009-07-01 13:52:18
date last changed
2017-10-01 03:47:25
@article{b187eb33-c6c8-4cf6-828a-f6c6022e5287,
  abstract     = {Process parameters for the extraction of glycolipids by scCO(2) from Lactobacillus plantarum B-01 were optimized by carrying out a two-level fractional design experiment. The highest glycolipids extract yield was obtained at 250 bar, 40 degrees C, CO2 flow rate, 10 g/min and hydrometanolic co-solvent, 9.3% (v/v). In the lipid extracts the carbohydrate moiety of glycolipids and in phospholipids phosphorus moiety were quantified and compared to the reference material isolated by classical organic solvent extraction. By TLC four groups of glycolipids were identified. A two step scCO(2) extraction process was established, whereby the second step yielded seven times more of glycolipids and four times more of phospholipids than in the extracts obtained with organic solvents. Prolonged scCO(2) extraction to 9 h resulted in 620 mu g of glycolipids and 875 mu g of phospholipids in a mg of extract in comparison to (1.5 h) 55 mu g and 15 mu g of glycolipids and phospholipids, respectively. (C) 2008 Elsevier B.V. All rights reserved.},
  author       = {Rakhuba, Dzianis and Novik, Galina and Dey, Estera},
  issn         = {0896-8446},
  keyword      = {Glycolipids,plantarum,Lactobacillus,Supercritical carbon dioxide,Lactic acid bacteria,Phospholipids,Neutral lipids},
  language     = {eng},
  number       = {1},
  pages        = {45--51},
  publisher    = {Elsevier},
  series       = {Journal of Supercritical Fluids},
  title        = {Application of supercritical carbon dioxide(scCO(2)) for the extraction of glycolipids from Lactobacillus plantarum B-01},
  url          = {http://dx.doi.org/10.1016/j.supflu.2008.11.016},
  volume       = {49},
  year         = {2009},
}