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Critical comparison of performances of superficially porous particles and sub-2 mu m particles under optimized ultra-high pressure conditions

Zhang, Yuan LU ; Wang, Xiaoli; Mukherjee, Partha and Petersson, Patrik (2009) In Journal of Chromatography A 1216(21). p.4597-4605
Abstract
The performance of 2.7 mu m superficially porous particles at 600 bar and sub-2 mu m fully porous particles at 1000 bar were compared by the Poppe plot method. Theoretical Poppe plots were first constructed for each stationary phase to compare their kinetic performance at different analysis times. The theory was then verified by experiments under the optimized conditions identified from the Poppe plot calculation. We found that the 2.7 mu m superficially porous particles at 600 bar can provide similar performance compared to the sub-2 mu m fully porous particles at ultra-high pressure (1000bar)when analysis times are very short (e.g. sub-minute). As analysis time increases, the superficially porous particles start to outperform the sub-2... (More)
The performance of 2.7 mu m superficially porous particles at 600 bar and sub-2 mu m fully porous particles at 1000 bar were compared by the Poppe plot method. Theoretical Poppe plots were first constructed for each stationary phase to compare their kinetic performance at different analysis times. The theory was then verified by experiments under the optimized conditions identified from the Poppe plot calculation. We found that the 2.7 mu m superficially porous particles at 600 bar can provide similar performance compared to the sub-2 mu m fully porous particles at ultra-high pressure (1000bar)when analysis times are very short (e.g. sub-minute). As analysis time increases, the superficially porous particles start to outperform the sub-2 mu m particles and can give much higher efficiencies (e.g. > 2 times higher plate Count) at very long analysis times (>3 h). The comparison was extended to gradient elution of a mixture of pharmaceutical interest by constructing gradient peak capacity Poppe plots and similar behavior was observed. (C) 2009 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ultra-high pressure, Sub-2 mu m particle, Superficially porous particle, Poppe plot, Performance comparison, Peak capacity, Fast LC
in
Journal of Chromatography A
volume
1216
issue
21
pages
4597 - 4605
publisher
Elsevier
external identifiers
  • wos:000266175100009
  • scopus:65249099055
ISSN
0021-9673
DOI
10.1016/j.chroma.2009.03.071
language
English
LU publication?
yes
id
9ba4b8ad-60ce-440b-aee3-c72d9cf8a4af (old id 1425636)
date added to LUP
2009-07-01 13:55:44
date last changed
2017-05-21 03:57:58
@article{9ba4b8ad-60ce-440b-aee3-c72d9cf8a4af,
  abstract     = {The performance of 2.7 mu m superficially porous particles at 600 bar and sub-2 mu m fully porous particles at 1000 bar were compared by the Poppe plot method. Theoretical Poppe plots were first constructed for each stationary phase to compare their kinetic performance at different analysis times. The theory was then verified by experiments under the optimized conditions identified from the Poppe plot calculation. We found that the 2.7 mu m superficially porous particles at 600 bar can provide similar performance compared to the sub-2 mu m fully porous particles at ultra-high pressure (1000bar)when analysis times are very short (e.g. sub-minute). As analysis time increases, the superficially porous particles start to outperform the sub-2 mu m particles and can give much higher efficiencies (e.g. > 2 times higher plate Count) at very long analysis times (>3 h). The comparison was extended to gradient elution of a mixture of pharmaceutical interest by constructing gradient peak capacity Poppe plots and similar behavior was observed. (C) 2009 Elsevier B.V. All rights reserved.},
  author       = {Zhang, Yuan and Wang, Xiaoli and Mukherjee, Partha and Petersson, Patrik},
  issn         = {0021-9673},
  keyword      = {Ultra-high pressure,Sub-2 mu m particle,Superficially porous particle,Poppe plot,Performance comparison,Peak capacity,Fast LC},
  language     = {eng},
  number       = {21},
  pages        = {4597--4605},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Critical comparison of performances of superficially porous particles and sub-2 mu m particles under optimized ultra-high pressure conditions},
  url          = {http://dx.doi.org/10.1016/j.chroma.2009.03.071},
  volume       = {1216},
  year         = {2009},
}