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Disposable Electrodes for Capacitive Immunosensor

Teeparuksapun, Kosin; Kanatharana, Proespichaya; Limbut, Warakorn; Thammakhet, Chongdee; Asawatreratanakul, Punnee; Mattiasson, Bo LU ; Wongkittisuksa, Booncharoen; Limsakul, Chusak and Thavarungkul, Panote (2009) In Electroanalysis 21(9). p.1066-1074
Abstract
Disposable electrodes were fabricated by coating chromium (5 nm) and gold (200 nm) on glass strips (5.0 mm x 25.4 mm) and used in a label-free immunosensor. Human Serum albumin (HSA) and its antigen (anti-HSA) were used as a model system. Electropolymerization of o-phenylenediamine was used for the immobilization of anti-HSA by covalent binding. A linear relationship was obtained in the range from 1.0 x 10-(14) to 1.0 x 10-(19) M with a limit of detection of 8.0 x 10(-15) M. Each modified electrode can be reused up to 30 times. The developed system was applied for human serum samples and compared to Albumin BCG method.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Capacitive immunosensor, o-Phenylenediamine, Disposable electrode, Thermal evaporation, Human serum albumin
in
Electroanalysis
volume
21
issue
9
pages
1066 - 1074
publisher
John Wiley & Sons
external identifiers
  • wos:000266153000009
  • scopus:66649135028
ISSN
1040-0397
DOI
10.1002/elan.200804517
language
English
LU publication?
yes
id
43c1f966-6df2-431d-9b4f-0ad8b2c7e023 (old id 1425688)
date added to LUP
2009-07-01 13:20:25
date last changed
2017-01-01 06:18:48
@article{43c1f966-6df2-431d-9b4f-0ad8b2c7e023,
  abstract     = {Disposable electrodes were fabricated by coating chromium (5 nm) and gold (200 nm) on glass strips (5.0 mm x 25.4 mm) and used in a label-free immunosensor. Human Serum albumin (HSA) and its antigen (anti-HSA) were used as a model system. Electropolymerization of o-phenylenediamine was used for the immobilization of anti-HSA by covalent binding. A linear relationship was obtained in the range from 1.0 x 10-(14) to 1.0 x 10-(19) M with a limit of detection of 8.0 x 10(-15) M. Each modified electrode can be reused up to 30 times. The developed system was applied for human serum samples and compared to Albumin BCG method.},
  author       = {Teeparuksapun, Kosin and Kanatharana, Proespichaya and Limbut, Warakorn and Thammakhet, Chongdee and Asawatreratanakul, Punnee and Mattiasson, Bo and Wongkittisuksa, Booncharoen and Limsakul, Chusak and Thavarungkul, Panote},
  issn         = {1040-0397},
  keyword      = {Capacitive immunosensor,o-Phenylenediamine,Disposable electrode,Thermal evaporation,Human serum albumin},
  language     = {eng},
  number       = {9},
  pages        = {1066--1074},
  publisher    = {John Wiley & Sons},
  series       = {Electroanalysis},
  title        = {Disposable Electrodes for Capacitive Immunosensor},
  url          = {http://dx.doi.org/10.1002/elan.200804517},
  volume       = {21},
  year         = {2009},
}