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Disposable Electrodes for Capacitive Immunosensor

Teeparuksapun, Kosin ; Kanatharana, Proespichaya ; Limbut, Warakorn ; Thammakhet, Chongdee ; Asawatreratanakul, Punnee ; Mattiasson, Bo LU ; Wongkittisuksa, Booncharoen ; Limsakul, Chusak and Thavarungkul, Panote (2009) In Electroanalysis 21(9). p.1066-1074
Abstract
Disposable electrodes were fabricated by coating chromium (5 nm) and gold (200 nm) on glass strips (5.0 mm x 25.4 mm) and used in a label-free immunosensor. Human Serum albumin (HSA) and its antigen (anti-HSA) were used as a model system. Electropolymerization of o-phenylenediamine was used for the immobilization of anti-HSA by covalent binding. A linear relationship was obtained in the range from 1.0 x 10-(14) to 1.0 x 10-(19) M with a limit of detection of 8.0 x 10(-15) M. Each modified electrode can be reused up to 30 times. The developed system was applied for human serum samples and compared to Albumin BCG method.
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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Capacitive immunosensor, o-Phenylenediamine, Disposable electrode, Thermal evaporation, Human serum albumin
in
Electroanalysis
volume
21
issue
9
pages
1066 - 1074
publisher
John Wiley & Sons Inc.
external identifiers
  • wos:000266153000009
  • scopus:66649135028
ISSN
1040-0397
DOI
10.1002/elan.200804517
language
English
LU publication?
yes
id
43c1f966-6df2-431d-9b4f-0ad8b2c7e023 (old id 1425688)
date added to LUP
2016-04-01 14:34:49
date last changed
2022-03-22 00:45:40
@article{43c1f966-6df2-431d-9b4f-0ad8b2c7e023,
  abstract     = {{Disposable electrodes were fabricated by coating chromium (5 nm) and gold (200 nm) on glass strips (5.0 mm x 25.4 mm) and used in a label-free immunosensor. Human Serum albumin (HSA) and its antigen (anti-HSA) were used as a model system. Electropolymerization of o-phenylenediamine was used for the immobilization of anti-HSA by covalent binding. A linear relationship was obtained in the range from 1.0 x 10-(14) to 1.0 x 10-(19) M with a limit of detection of 8.0 x 10(-15) M. Each modified electrode can be reused up to 30 times. The developed system was applied for human serum samples and compared to Albumin BCG method.}},
  author       = {{Teeparuksapun, Kosin and Kanatharana, Proespichaya and Limbut, Warakorn and Thammakhet, Chongdee and Asawatreratanakul, Punnee and Mattiasson, Bo and Wongkittisuksa, Booncharoen and Limsakul, Chusak and Thavarungkul, Panote}},
  issn         = {{1040-0397}},
  keywords     = {{Capacitive immunosensor; o-Phenylenediamine; Disposable electrode; Thermal evaporation; Human serum albumin}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{1066--1074}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Electroanalysis}},
  title        = {{Disposable Electrodes for Capacitive Immunosensor}},
  url          = {{http://dx.doi.org/10.1002/elan.200804517}},
  doi          = {{10.1002/elan.200804517}},
  volume       = {{21}},
  year         = {{2009}},
}