Efficient production of mesencephalic dopamine neurons by Lmx1a expression in embryonic stem cells
(2009) In Proceedings of the National Academy of Sciences 106(18). p.7613-7618- Abstract
- Signaling factors involved in CNS development have been used to control the differentiation of embryonic stem cells (ESCs) into mesencephalic dopamine (mesDA) neurons, but tend to generate a limited yield of desired cell type. Here we show that forced expression of Lmx1a, a transcription factor functioning as a determinant of mesDA neurons during embryogenesis, effectively can promote the generation of mesDA neurons from mouse and human ESCs. Under permissive culture conditions, 75%-95% of mouse ESC-derived neurons express molecular and physiological properties characteristic of bona fide mesDA neurons. Similar to primary mesDA neurons, these cells integrate and innervate the striatum of 6-hydroxy dopamine lesioned neonatal rats. Thus, the... (More)
- Signaling factors involved in CNS development have been used to control the differentiation of embryonic stem cells (ESCs) into mesencephalic dopamine (mesDA) neurons, but tend to generate a limited yield of desired cell type. Here we show that forced expression of Lmx1a, a transcription factor functioning as a determinant of mesDA neurons during embryogenesis, effectively can promote the generation of mesDA neurons from mouse and human ESCs. Under permissive culture conditions, 75%-95% of mouse ESC-derived neurons express molecular and physiological properties characteristic of bona fide mesDA neurons. Similar to primary mesDA neurons, these cells integrate and innervate the striatum of 6-hydroxy dopamine lesioned neonatal rats. Thus, the enriched generation of functional mesDA neurons by forced expression of Lmx1a may be of future importance in cell replacement therapy of Parkinson disease. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1426303
- author
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Parkinson, neuroscience, intrinsic determinant, magnetic bead sorting, disease, transplantation
- in
- Proceedings of the National Academy of Sciences
- volume
- 106
- issue
- 18
- pages
- 7613 - 7618
- publisher
- National Academy of Sciences
- external identifiers
-
- wos:000265783600063
- scopus:66149085781
- pmid:19383789
- ISSN
- 1091-6490
- DOI
- 10.1073/pnas.0902396106
- language
- English
- LU publication?
- yes
- id
- 03561632-4498-4509-a4f9-8894cbb1aa80 (old id 1426303)
- date added to LUP
- 2016-04-01 11:49:53
- date last changed
- 2022-03-20 19:33:39
@article{03561632-4498-4509-a4f9-8894cbb1aa80, abstract = {{Signaling factors involved in CNS development have been used to control the differentiation of embryonic stem cells (ESCs) into mesencephalic dopamine (mesDA) neurons, but tend to generate a limited yield of desired cell type. Here we show that forced expression of Lmx1a, a transcription factor functioning as a determinant of mesDA neurons during embryogenesis, effectively can promote the generation of mesDA neurons from mouse and human ESCs. Under permissive culture conditions, 75%-95% of mouse ESC-derived neurons express molecular and physiological properties characteristic of bona fide mesDA neurons. Similar to primary mesDA neurons, these cells integrate and innervate the striatum of 6-hydroxy dopamine lesioned neonatal rats. Thus, the enriched generation of functional mesDA neurons by forced expression of Lmx1a may be of future importance in cell replacement therapy of Parkinson disease.}}, author = {{Friling, Stina and Andersson, Elisabet and Thompson, Lachlan and Jönsson, Marie and Hebsgaard, Josephine and Nanou, Evanthia and Alekseenko, Zhanna and Marklund, Ulrika and Kjellander, Susanna and Volakakis, Nikolaos and Hovatta, Outi and El Manira, Abdeljabbar and Björklund, Anders and Perlmann, Thomas and Ericson, Johan}}, issn = {{1091-6490}}, keywords = {{Parkinson; neuroscience; intrinsic determinant; magnetic bead sorting; disease; transplantation}}, language = {{eng}}, number = {{18}}, pages = {{7613--7618}}, publisher = {{National Academy of Sciences}}, series = {{Proceedings of the National Academy of Sciences}}, title = {{Efficient production of mesencephalic dopamine neurons by Lmx1a expression in embryonic stem cells}}, url = {{http://dx.doi.org/10.1073/pnas.0902396106}}, doi = {{10.1073/pnas.0902396106}}, volume = {{106}}, year = {{2009}}, }