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Capacitive biosensor for quantification of trace amounts of DNA

Numnuam, Apon; Kanatharana, Proespichaya; Mattiasson, Bo LU ; Asawatreratanakul, Punnee; Wongkittisuksa, Booncharoen; Limsakul, Chusak and Thavarungkul, Panote (2009) In Biosensors & Bioelectronics 24(8). p.2559-2565
Abstract
A flow injection capacitive biosensor system to detect trace amounts DNA has been developed based on the affinity binding between immobilized histone and DNA. Histones from calf thymus and shrimp were immobilized on gold electrodes covered with self-assembled monolayer (SAM) of thioctic acid. Each of these histones was used to detect DNA from calf thymus, shrimp and Escherichia coli. The studies indicated that histones can bind better with DNA from the same source and give higher sensitivity than the binding with DNA from different sources. Under optimum conditions, both histones from calf thymus and shrimp provided the same lower detection limit of 10(-5) ng l(-1) for DNA from different sources, i.e., calf thymus, shrimp and E. coli. The... (More)
A flow injection capacitive biosensor system to detect trace amounts DNA has been developed based on the affinity binding between immobilized histone and DNA. Histones from calf thymus and shrimp were immobilized on gold electrodes covered with self-assembled monolayer (SAM) of thioctic acid. Each of these histones was used to detect DNA from calf thymus, shrimp and Escherichia coli. The studies indicated that histones can bind better with DNA from the same source and give higher sensitivity than the binding with DNA from different sources. Under optimum conditions, both histones from calf thymus and shrimp provided the same lower detection limit of 10(-5) ng l(-1) for DNA from different sources, i.e., calf thymus, shrimp and E. coli. The standard curve for the affinity reaction between calf thymus histone and DNA shows sigmoidal behavior and two linear ranges, 10(-5) to 10(-2) ng l(-1) and 10(-1) to 10(2) ng l(-1), could be obtained. The immobilized histones were stable and after regeneration good reproducibility of the signal could be obtained up to 43 times with a %R.S.D. of 3.1. When applied to analyze residual DNA in crude protein extracted from white shrimp recoveries were obtained between 80% and 116%. (c) 2009 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Self-assembled monolayer, Capacitive biosensor, Histone, SAM, DNA-assay
in
Biosensors & Bioelectronics
volume
24
issue
8
pages
2559 - 2565
publisher
Elsevier
external identifiers
  • wos:000265811000041
  • scopus:62749083856
ISSN
1873-4235
DOI
10.1016/j.bios.2009.01.005
language
English
LU publication?
yes
id
43e6dfbe-4102-424b-b44b-70d65cb118ca (old id 1426444)
date added to LUP
2009-06-29 15:15:05
date last changed
2017-01-01 06:13:51
@article{43e6dfbe-4102-424b-b44b-70d65cb118ca,
  abstract     = {A flow injection capacitive biosensor system to detect trace amounts DNA has been developed based on the affinity binding between immobilized histone and DNA. Histones from calf thymus and shrimp were immobilized on gold electrodes covered with self-assembled monolayer (SAM) of thioctic acid. Each of these histones was used to detect DNA from calf thymus, shrimp and Escherichia coli. The studies indicated that histones can bind better with DNA from the same source and give higher sensitivity than the binding with DNA from different sources. Under optimum conditions, both histones from calf thymus and shrimp provided the same lower detection limit of 10(-5) ng l(-1) for DNA from different sources, i.e., calf thymus, shrimp and E. coli. The standard curve for the affinity reaction between calf thymus histone and DNA shows sigmoidal behavior and two linear ranges, 10(-5) to 10(-2) ng l(-1) and 10(-1) to 10(2) ng l(-1), could be obtained. The immobilized histones were stable and after regeneration good reproducibility of the signal could be obtained up to 43 times with a %R.S.D. of 3.1. When applied to analyze residual DNA in crude protein extracted from white shrimp recoveries were obtained between 80% and 116%. (c) 2009 Elsevier B.V. All rights reserved.},
  author       = {Numnuam, Apon and Kanatharana, Proespichaya and Mattiasson, Bo and Asawatreratanakul, Punnee and Wongkittisuksa, Booncharoen and Limsakul, Chusak and Thavarungkul, Panote},
  issn         = {1873-4235},
  keyword      = {Self-assembled monolayer,Capacitive biosensor,Histone,SAM,DNA-assay},
  language     = {eng},
  number       = {8},
  pages        = {2559--2565},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Capacitive biosensor for quantification of trace amounts of DNA},
  url          = {http://dx.doi.org/10.1016/j.bios.2009.01.005},
  volume       = {24},
  year         = {2009},
}