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Chromato-panning: an efficient new mode of identifying suitable ligands from phage display libraries

Noppe, Wim LU ; Plieva, Fatima LU ; Galaev, Igor LU ; Pottel, Hans; Deckmyn, Hans and Mattiasson, Bo LU (2009) In BMC Biotechnology 9.
Abstract
Background: Phage Display technology is a well established technique for high throughput screening of affinity ligands. Here we describe a new compact chromato-panning procedure for selection of suitable binders from a phage peptide display library. Results: Both phages and E. coli cells pass non-hindered through the interconnected pores of macroporous gel, so called cryogel. After coupling a ligand to a monolithic cryogel column, the phage library was applied on the column and non-bound phages were washed out. The selection of strong phage-binders was achieved already after the first panning cycle due to the efficient separation of phage-binders from phage-non-binders in chromatographic mode rather than in batch mode as in traditional... (More)
Background: Phage Display technology is a well established technique for high throughput screening of affinity ligands. Here we describe a new compact chromato-panning procedure for selection of suitable binders from a phage peptide display library. Results: Both phages and E. coli cells pass non-hindered through the interconnected pores of macroporous gel, so called cryogel. After coupling a ligand to a monolithic cryogel column, the phage library was applied on the column and non-bound phages were washed out. The selection of strong phage-binders was achieved already after the first panning cycle due to the efficient separation of phage-binders from phage-non-binders in chromatographic mode rather than in batch mode as in traditional biopanning procedures. E. coli cells were applied on the column for infection with the specifically bound phages. Conclusion: Chromato-panning allows combining several steps of the panning procedure resulting in 4-8 fold decrease of total time needed for phage selection. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
BMC Biotechnology
volume
9
publisher
BioMed Central
external identifiers
  • wos:000265608000001
  • scopus:64849104120
ISSN
1472-6750
DOI
10.1186/1472-6750-9-21
language
English
LU publication?
yes
id
c02feb63-0c75-498d-9b63-6043d6df0ab2 (old id 1428033)
date added to LUP
2009-06-25 10:56:13
date last changed
2017-09-24 04:01:00
@article{c02feb63-0c75-498d-9b63-6043d6df0ab2,
  abstract     = {Background: Phage Display technology is a well established technique for high throughput screening of affinity ligands. Here we describe a new compact chromato-panning procedure for selection of suitable binders from a phage peptide display library. Results: Both phages and E. coli cells pass non-hindered through the interconnected pores of macroporous gel, so called cryogel. After coupling a ligand to a monolithic cryogel column, the phage library was applied on the column and non-bound phages were washed out. The selection of strong phage-binders was achieved already after the first panning cycle due to the efficient separation of phage-binders from phage-non-binders in chromatographic mode rather than in batch mode as in traditional biopanning procedures. E. coli cells were applied on the column for infection with the specifically bound phages. Conclusion: Chromato-panning allows combining several steps of the panning procedure resulting in 4-8 fold decrease of total time needed for phage selection.},
  author       = {Noppe, Wim and Plieva, Fatima and Galaev, Igor and Pottel, Hans and Deckmyn, Hans and Mattiasson, Bo},
  issn         = {1472-6750},
  language     = {eng},
  publisher    = {BioMed Central},
  series       = {BMC Biotechnology},
  title        = {Chromato-panning: an efficient new mode of identifying suitable ligands from phage display libraries},
  url          = {http://dx.doi.org/10.1186/1472-6750-9-21},
  volume       = {9},
  year         = {2009},
}