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Amperometric Biosensor For Ethanol Based On A Phenothiazine Derivative Modified Carbon Paste Electrode

Gligor, Delia; Csöregi, Elisabeth LU and Popescu, Ionel Catalin (2008) In Studia Universitatis Babes-Bolyai, Chemia 53(1). p.55-62
Abstract
A new amperometric biosensor for ethanol, based on carbon paste electrode modified with alcohol dehydrogenase (ADH), polyethylenimine (PEI) and using a phenothiazine derivative (DDDP; 16H,18H-dibenzo[c,1]7,9-dithia-16,18-diazapentacene) as redox mediator for NADH recycling, was developed. The biosensor response is the result of mediated oxidation of NADH, generated in the enzymatic reaction between ADH and ethanol (in the presence of NAD(+)). The biosensor sensitivity (calculated as the ratio I-max/K-M(app)) was 0.035 mA M-1 and the detection limit was 0.26 mM, while the linear response range was from 0.1 to 20 mM ethanol.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
phenothiazine derivative, adenine dinucleotide, nicotinamide, ethanol, amperometric biosensors, alcohol dehydrogenase, polyethyleneimine, modified carbon paste electrodes
in
Studia Universitatis Babes-Bolyai, Chemia
volume
53
issue
1
pages
55 - 62
publisher
Univ Babes-Bolyai
external identifiers
  • wos:000266403700008
ISSN
2065-9520
language
English
LU publication?
yes
id
0d8ef4f3-c39c-43ae-8b08-afd57cc56e65 (old id 1428191)
date added to LUP
2009-06-25 10:53:36
date last changed
2016-04-15 19:53:34
@article{0d8ef4f3-c39c-43ae-8b08-afd57cc56e65,
  abstract     = {A new amperometric biosensor for ethanol, based on carbon paste electrode modified with alcohol dehydrogenase (ADH), polyethylenimine (PEI) and using a phenothiazine derivative (DDDP; 16H,18H-dibenzo[c,1]7,9-dithia-16,18-diazapentacene) as redox mediator for NADH recycling, was developed. The biosensor response is the result of mediated oxidation of NADH, generated in the enzymatic reaction between ADH and ethanol (in the presence of NAD(+)). The biosensor sensitivity (calculated as the ratio I-max/K-M(app)) was 0.035 mA M-1 and the detection limit was 0.26 mM, while the linear response range was from 0.1 to 20 mM ethanol.},
  author       = {Gligor, Delia and Csöregi, Elisabeth and Popescu, Ionel Catalin},
  issn         = {2065-9520},
  keyword      = {phenothiazine derivative,adenine dinucleotide,nicotinamide,ethanol,amperometric biosensors,alcohol dehydrogenase,polyethyleneimine,modified carbon paste electrodes},
  language     = {eng},
  number       = {1},
  pages        = {55--62},
  publisher    = {Univ Babes-Bolyai},
  series       = {Studia Universitatis Babes-Bolyai, Chemia},
  title        = {Amperometric Biosensor For Ethanol Based On A Phenothiazine Derivative Modified Carbon Paste Electrode},
  volume       = {53},
  year         = {2008},
}