Advanced

AMPEROMETRIC BIOSENSORS FOR GLUCOSE AND ETHANOL DETERMINATION IN WINE USING FLOW INJECTION ANALYSIS

Muresan, Laura LU ; Zor, Kinga LU ; Nistor, Mihaela LU ; Csöregi, Elisabeth LU and Popescu, Ionel Catalin (2008) In Studia Universitatis Babes-Bolyai, Chemia 53(1). p.71-79
Abstract
Reagentless amperometric biosensors for glucose and ethanol were developed and successfully applied for monitoring glucose and ethanol concentrations in wine during the fermentation process. The glucose biosensor was based on commercially available glucose oxidase and horseradish peroxidase co-immobilized on solid graphite using Os(II)-redox hydrogel (RH) [1]. In the case of ethanol biosensor, the quinohemoprotein dependent alcohol dehydrogenase was immobilized on the graphite electrode surface using the same RH [2]. Both biosensors were operated at low applied potentials (-50 mV vs. Ag/AgCl, KCl0.1M for glucose biosensor, and +250 mV vs. Ag/AgCl, KCl0.1M for ethanol biosensor), where biases from interferences are minimal. The... (More)
Reagentless amperometric biosensors for glucose and ethanol were developed and successfully applied for monitoring glucose and ethanol concentrations in wine during the fermentation process. The glucose biosensor was based on commercially available glucose oxidase and horseradish peroxidase co-immobilized on solid graphite using Os(II)-redox hydrogel (RH) [1]. In the case of ethanol biosensor, the quinohemoprotein dependent alcohol dehydrogenase was immobilized on the graphite electrode surface using the same RH [2]. Both biosensors were operated at low applied potentials (-50 mV vs. Ag/AgCl, KCl0.1M for glucose biosensor, and +250 mV vs. Ag/AgCl, KCl0.1M for ethanol biosensor), where biases from interferences are minimal. The bioelectroanalytical parameters, estimated from flow injection analysis measurements, were found as follows: sensitivity, 0.73 +/- 0.01 mu A mM(-1) for glucose and 0.45 +/- 0.01 mu A mM(-1) for ethanol; linear range up to 1 mM in both cases; detection limit, 7.0 mu M for glucose and 8.9 mu M for ethanol. The results for real samples were found in good agreement with those reported by Barsan et al. [3]. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
flow injection analysis, amperometric biosensors, ethanol, glucose, wine
in
Studia Universitatis Babes-Bolyai, Chemia
volume
53
issue
1
pages
71 - 79
publisher
Univ Babes-Bolyai
external identifiers
  • wos:000266403700010
ISSN
2065-9520
language
English
LU publication?
yes
id
1fb9bafd-6c3f-4bb9-9ad6-d37e259f292b (old id 1428195)
date added to LUP
2009-06-25 10:54:39
date last changed
2016-04-15 20:17:36
@article{1fb9bafd-6c3f-4bb9-9ad6-d37e259f292b,
  abstract     = {Reagentless amperometric biosensors for glucose and ethanol were developed and successfully applied for monitoring glucose and ethanol concentrations in wine during the fermentation process. The glucose biosensor was based on commercially available glucose oxidase and horseradish peroxidase co-immobilized on solid graphite using Os(II)-redox hydrogel (RH) [1]. In the case of ethanol biosensor, the quinohemoprotein dependent alcohol dehydrogenase was immobilized on the graphite electrode surface using the same RH [2]. Both biosensors were operated at low applied potentials (-50 mV vs. Ag/AgCl, KCl0.1M for glucose biosensor, and +250 mV vs. Ag/AgCl, KCl0.1M for ethanol biosensor), where biases from interferences are minimal. The bioelectroanalytical parameters, estimated from flow injection analysis measurements, were found as follows: sensitivity, 0.73 +/- 0.01 mu A mM(-1) for glucose and 0.45 +/- 0.01 mu A mM(-1) for ethanol; linear range up to 1 mM in both cases; detection limit, 7.0 mu M for glucose and 8.9 mu M for ethanol. The results for real samples were found in good agreement with those reported by Barsan et al. [3].},
  author       = {Muresan, Laura and Zor, Kinga and Nistor, Mihaela and Csöregi, Elisabeth and Popescu, Ionel Catalin},
  issn         = {2065-9520},
  keyword      = {flow injection analysis,amperometric biosensors,ethanol,glucose,wine},
  language     = {eng},
  number       = {1},
  pages        = {71--79},
  publisher    = {Univ Babes-Bolyai},
  series       = {Studia Universitatis Babes-Bolyai, Chemia},
  title        = {AMPEROMETRIC BIOSENSORS FOR GLUCOSE AND ETHANOL DETERMINATION IN WINE USING FLOW INJECTION ANALYSIS},
  volume       = {53},
  year         = {2008},
}