AMPEROMETRIC BIOSENSORS FOR GLUCOSE AND ETHANOL DETERMINATION IN WINE USING FLOW INJECTION ANALYSIS
(2008) In Studia Universitatis Babes-Bolyai, Chemia 53(1). p.71-79- Abstract
- Reagentless amperometric biosensors for glucose and ethanol were developed and successfully applied for monitoring glucose and ethanol concentrations in wine during the fermentation process. The glucose biosensor was based on commercially available glucose oxidase and horseradish peroxidase co-immobilized on solid graphite using Os(II)-redox hydrogel (RH) [1]. In the case of ethanol biosensor, the quinohemoprotein dependent alcohol dehydrogenase was immobilized on the graphite electrode surface using the same RH [2]. Both biosensors were operated at low applied potentials (-50 mV vs. Ag/AgCl, KCl0.1M for glucose biosensor, and +250 mV vs. Ag/AgCl, KCl0.1M for ethanol biosensor), where biases from interferences are minimal. The... (More)
- Reagentless amperometric biosensors for glucose and ethanol were developed and successfully applied for monitoring glucose and ethanol concentrations in wine during the fermentation process. The glucose biosensor was based on commercially available glucose oxidase and horseradish peroxidase co-immobilized on solid graphite using Os(II)-redox hydrogel (RH) [1]. In the case of ethanol biosensor, the quinohemoprotein dependent alcohol dehydrogenase was immobilized on the graphite electrode surface using the same RH [2]. Both biosensors were operated at low applied potentials (-50 mV vs. Ag/AgCl, KCl0.1M for glucose biosensor, and +250 mV vs. Ag/AgCl, KCl0.1M for ethanol biosensor), where biases from interferences are minimal. The bioelectroanalytical parameters, estimated from flow injection analysis measurements, were found as follows: sensitivity, 0.73 +/- 0.01 mu A mM(-1) for glucose and 0.45 +/- 0.01 mu A mM(-1) for ethanol; linear range up to 1 mM in both cases; detection limit, 7.0 mu M for glucose and 8.9 mu M for ethanol. The results for real samples were found in good agreement with those reported by Barsan et al. [3]. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1428195
- author
- Muresan, Laura LU ; Zor, Kinga LU ; Nistor, Mihaela LU ; Csöregi, Elisabeth LU and Popescu, Ionel Catalin
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- flow injection analysis, amperometric biosensors, ethanol, glucose, wine
- in
- Studia Universitatis Babes-Bolyai, Chemia
- volume
- 53
- issue
- 1
- pages
- 71 - 79
- publisher
- Universitatea Babes-Bolyai
- external identifiers
-
- wos:000266403700010
- ISSN
- 2065-9520
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 1fb9bafd-6c3f-4bb9-9ad6-d37e259f292b (old id 1428195)
- date added to LUP
- 2016-04-01 12:22:50
- date last changed
- 2018-11-21 20:06:50
@article{1fb9bafd-6c3f-4bb9-9ad6-d37e259f292b, abstract = {{Reagentless amperometric biosensors for glucose and ethanol were developed and successfully applied for monitoring glucose and ethanol concentrations in wine during the fermentation process. The glucose biosensor was based on commercially available glucose oxidase and horseradish peroxidase co-immobilized on solid graphite using Os(II)-redox hydrogel (RH) [1]. In the case of ethanol biosensor, the quinohemoprotein dependent alcohol dehydrogenase was immobilized on the graphite electrode surface using the same RH [2]. Both biosensors were operated at low applied potentials (-50 mV vs. Ag/AgCl, KCl0.1M for glucose biosensor, and +250 mV vs. Ag/AgCl, KCl0.1M for ethanol biosensor), where biases from interferences are minimal. The bioelectroanalytical parameters, estimated from flow injection analysis measurements, were found as follows: sensitivity, 0.73 +/- 0.01 mu A mM(-1) for glucose and 0.45 +/- 0.01 mu A mM(-1) for ethanol; linear range up to 1 mM in both cases; detection limit, 7.0 mu M for glucose and 8.9 mu M for ethanol. The results for real samples were found in good agreement with those reported by Barsan et al. [3].}}, author = {{Muresan, Laura and Zor, Kinga and Nistor, Mihaela and Csöregi, Elisabeth and Popescu, Ionel Catalin}}, issn = {{2065-9520}}, keywords = {{flow injection analysis; amperometric biosensors; ethanol; glucose; wine}}, language = {{eng}}, number = {{1}}, pages = {{71--79}}, publisher = {{Universitatea Babes-Bolyai}}, series = {{Studia Universitatis Babes-Bolyai, Chemia}}, title = {{AMPEROMETRIC BIOSENSORS FOR GLUCOSE AND ETHANOL DETERMINATION IN WINE USING FLOW INJECTION ANALYSIS}}, volume = {{53}}, year = {{2008}}, }