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Kinetic Modeling of Charge-Transfer Quenching in the CP29 Minor Complex

Cheng, Y. C.; Ahn, T. K.; Avenson, T. J.; Zigmantas, Donatas LU ; Niyogi, K. K.; Ballottari, M.; Bassi, R. and Fleming, G. R. (2008) In The Journal of Physical Chemistry Part B 112(42). p.13418-13423
Abstract
We performed transient absorption (TA) measurements on CP29 minor light-harvesting complexes that were reconstituted in vitro with either violaxanthin (Vio) or zeaxanthin (Zea) and demonstrate that the Zea-bound CP29 complexes exhibit charge-transfer (CT) quenching that has been correlated with the energy-dependent quenching (qE) in higher plants. Simulations of the difference TA kinetics reveal two-phase kinetics for intracomplex energy transfer to the CT quenching site in CP29 complexes, with a fast <500 fs component and a similar to 6 ps component. Specific chlorophyll sites within CP29 are identified as likely locations for CT quenching. We also construct a kinetic model for CT quenching during qE in an intact system that... (More)
We performed transient absorption (TA) measurements on CP29 minor light-harvesting complexes that were reconstituted in vitro with either violaxanthin (Vio) or zeaxanthin (Zea) and demonstrate that the Zea-bound CP29 complexes exhibit charge-transfer (CT) quenching that has been correlated with the energy-dependent quenching (qE) in higher plants. Simulations of the difference TA kinetics reveal two-phase kinetics for intracomplex energy transfer to the CT quenching site in CP29 complexes, with a fast <500 fs component and a similar to 6 ps component. Specific chlorophyll sites within CP29 are identified as likely locations for CT quenching. We also construct a kinetic model for CT quenching during qE in an intact system that incorporates CP29 as a CT trap and show that the model is consistent with previous in vivo measurements on spinach thylakoid membranes. Finally, we compare simulations of CT quenching in thylakoids with those of the individual CP29 complexes and propose that CP29 rather than LHCII is a site of CT quenching. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
lhcii complex, peak shift, excited-states, cation formation, chlorophyll fluorescence, green plants, energy-transfer, plant antenna protein, photosystem-ii complex, light-harvesting complex
in
The Journal of Physical Chemistry Part B
volume
112
issue
42
pages
13418 - 13423
publisher
The American Chemical Society
external identifiers
  • scopus:55649100322
ISSN
1520-5207
DOI
10.1021/jp802730c
language
English
LU publication?
no
id
fc7d5cea-e403-4bb0-84db-ad45ab08ab61 (old id 1432930)
date added to LUP
2009-07-02 14:40:57
date last changed
2017-01-15 04:01:06
@article{fc7d5cea-e403-4bb0-84db-ad45ab08ab61,
  abstract     = {We performed transient absorption (TA) measurements on CP29 minor light-harvesting complexes that were reconstituted in vitro with either violaxanthin (Vio) or zeaxanthin (Zea) and demonstrate that the Zea-bound CP29 complexes exhibit charge-transfer (CT) quenching that has been correlated with the energy-dependent quenching (qE) in higher plants. Simulations of the difference TA kinetics reveal two-phase kinetics for intracomplex energy transfer to the CT quenching site in CP29 complexes, with a fast &lt;500 fs component and a similar to 6 ps component. Specific chlorophyll sites within CP29 are identified as likely locations for CT quenching. We also construct a kinetic model for CT quenching during qE in an intact system that incorporates CP29 as a CT trap and show that the model is consistent with previous in vivo measurements on spinach thylakoid membranes. Finally, we compare simulations of CT quenching in thylakoids with those of the individual CP29 complexes and propose that CP29 rather than LHCII is a site of CT quenching.},
  author       = {Cheng, Y. C. and Ahn, T. K. and Avenson, T. J. and Zigmantas, Donatas and Niyogi, K. K. and Ballottari, M. and Bassi, R. and Fleming, G. R.},
  issn         = {1520-5207},
  keyword      = {lhcii complex,peak shift,excited-states,cation formation,chlorophyll fluorescence,green plants,energy-transfer,plant antenna protein,photosystem-ii complex,light-harvesting complex},
  language     = {eng},
  number       = {42},
  pages        = {13418--13423},
  publisher    = {The American Chemical Society},
  series       = {The Journal of Physical Chemistry Part B},
  title        = {Kinetic Modeling of Charge-Transfer Quenching in the CP29 Minor Complex},
  url          = {http://dx.doi.org/10.1021/jp802730c},
  volume       = {112},
  year         = {2008},
}