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A multipurpose capacitive biosensor for assay and quality control of human immunoglobulin G.

Labib, Mahmoud LU ; Hedström, Martin LU ; Amin, Magdy and Mattiasson, Bo LU (2009) In Biotechnology and Bioengineering 104. p.312-320
Abstract
We report a flow-injection biosensor system with a capacitive transducer for assay and quality control of human immunoglobulin G (hIgG). The sensing platform is based on self-assembled monolayers (SAMs) of carboxylic acid terminated alkyl-thiols with covalently attached concanavalin A. The electrochemical characteristics of the sensor surface were assessed by cyclic voltammetry using a permeable redox couple (potassium ferricyanide). The developed biosensor proved capable of performing a sensitive label-free assay of hIgG with a detection limit of 1.0 microg mL(-1). The capacitance response depended linearly on hIgG concentration over the range from 5.0 to 100 microg mL(-1), in a logarithmic plot. Typical measurements were performed in 15... (More)
We report a flow-injection biosensor system with a capacitive transducer for assay and quality control of human immunoglobulin G (hIgG). The sensing platform is based on self-assembled monolayers (SAMs) of carboxylic acid terminated alkyl-thiols with covalently attached concanavalin A. The electrochemical characteristics of the sensor surface were assessed by cyclic voltammetry using a permeable redox couple (potassium ferricyanide). The developed biosensor proved capable of performing a sensitive label-free assay of hIgG with a detection limit of 1.0 microg mL(-1). The capacitance response depended linearly on hIgG concentration over the range from 5.0 to 100 microg mL(-1), in a logarithmic plot. Typical measurements were performed in 15 min and up to 18 successive assays were achieved without significant loss of sensitivity using a single electrode. In addition, the biosensor can detect hIgG aggregates with concentrations as low as 0.01% of the total hIgG content (5.0 microg mL(-1)). Hence, it represents a potential post-size-exclusion chromatography-UV (post-SEC-UV) binding assay for in-process quality control of hIgG, which cannot be detected by SEC-UV singly at concentrations below 0.3% of the total hIgG content. Biotechnol. Bioeng. (c) 2009 Wiley Periodicals, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology and Bioengineering
volume
104
pages
312 - 320
publisher
John Wiley & Sons
external identifiers
  • wos:000269846900009
  • pmid:19562733
  • scopus:70349329559
ISSN
1097-0290
DOI
10.1002/bit.22395
language
English
LU publication?
yes
id
7f5fa750-883b-45cf-b9e3-929b17d4f0e0 (old id 1433763)
date added to LUP
2009-07-03 09:16:32
date last changed
2017-09-24 03:27:58
@article{7f5fa750-883b-45cf-b9e3-929b17d4f0e0,
  abstract     = {We report a flow-injection biosensor system with a capacitive transducer for assay and quality control of human immunoglobulin G (hIgG). The sensing platform is based on self-assembled monolayers (SAMs) of carboxylic acid terminated alkyl-thiols with covalently attached concanavalin A. The electrochemical characteristics of the sensor surface were assessed by cyclic voltammetry using a permeable redox couple (potassium ferricyanide). The developed biosensor proved capable of performing a sensitive label-free assay of hIgG with a detection limit of 1.0 microg mL(-1). The capacitance response depended linearly on hIgG concentration over the range from 5.0 to 100 microg mL(-1), in a logarithmic plot. Typical measurements were performed in 15 min and up to 18 successive assays were achieved without significant loss of sensitivity using a single electrode. In addition, the biosensor can detect hIgG aggregates with concentrations as low as 0.01% of the total hIgG content (5.0 microg mL(-1)). Hence, it represents a potential post-size-exclusion chromatography-UV (post-SEC-UV) binding assay for in-process quality control of hIgG, which cannot be detected by SEC-UV singly at concentrations below 0.3% of the total hIgG content. Biotechnol. Bioeng. (c) 2009 Wiley Periodicals, Inc.},
  author       = {Labib, Mahmoud and Hedström, Martin and Amin, Magdy and Mattiasson, Bo},
  issn         = {1097-0290},
  language     = {eng},
  pages        = {312--320},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {A multipurpose capacitive biosensor for assay and quality control of human immunoglobulin G.},
  url          = {http://dx.doi.org/10.1002/bit.22395},
  volume       = {104},
  year         = {2009},
}