Proteolytic degradation of human salivary MUC5B by dental biofilms.
(2009) In Microbiology- Abstract
- The degradation of complex substrates, like salivary mucins, requires an arsenal of glycosidases and proteases to sequentially degrade the oligosaccharides and polypeptide backbone. MUC5B is a complex oligomeric glycoprotein, heterogeneous in molecular mass (14-40 x 106 Da), with a diverse repertoire of oligosaccharides, differing in composition and charge. The aim of this study was to investigate whether proteolytic degradation of the mucin polypeptide backbone could be identified and if cooperation of dental biofilm bacteria was required. Cooperative bacterial-mediated proteolysis of MUC5B was determined by comparing individual and mixed consortia of strains isolated from supragingival plaque and freshly harvested supragingival plaque.... (More)
- The degradation of complex substrates, like salivary mucins, requires an arsenal of glycosidases and proteases to sequentially degrade the oligosaccharides and polypeptide backbone. MUC5B is a complex oligomeric glycoprotein, heterogeneous in molecular mass (14-40 x 106 Da), with a diverse repertoire of oligosaccharides, differing in composition and charge. The aim of this study was to investigate whether proteolytic degradation of the mucin polypeptide backbone could be identified and if cooperation of dental biofilm bacteria was required. Cooperative bacterial-mediated proteolysis of MUC5B was determined by comparing individual and mixed consortia of strains isolated from supragingival plaque and freshly harvested supragingival plaque. Proteolytic activity was analyzed using fluorescent labelled substrate and by visualizing mucin degradation by SDS-PAGE. Dental plaque degraded the polypeptide backbone of the salivary MUC5B mucin. The mucin also was degraded by a specific consortium of isolated species from supragingival plaque, although individual species and other consortia did not. Select bacteria in supragingival dental plaque, therefore, cooperate as a consortium to proteolyze human salivary MUC5B and hydrolyze glycosides. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1433878
- author
- Wickström, Claes LU ; Herzberg, Mark C ; Beighton, David and Svensater, Gunnel
- organization
- publishing date
- 2009-06-25
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Microbiology
- publisher
- MAIK Nauka/Interperiodica
- external identifiers
-
- pmid:19556293
- scopus:69949129819
- pmid:19556293
- ISSN
- 1465-2080
- DOI
- 10.1099/mic.0.030536-0
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Mucosal biology (013212033)
- id
- f2dfe253-2d45-4b6a-b0ed-be1f730a644c (old id 1433878)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/19556293?dopt=Abstract
- date added to LUP
- 2016-04-04 07:08:01
- date last changed
- 2022-02-05 22:12:39
@article{f2dfe253-2d45-4b6a-b0ed-be1f730a644c,
abstract = {{The degradation of complex substrates, like salivary mucins, requires an arsenal of glycosidases and proteases to sequentially degrade the oligosaccharides and polypeptide backbone. MUC5B is a complex oligomeric glycoprotein, heterogeneous in molecular mass (14-40 x 106 Da), with a diverse repertoire of oligosaccharides, differing in composition and charge. The aim of this study was to investigate whether proteolytic degradation of the mucin polypeptide backbone could be identified and if cooperation of dental biofilm bacteria was required. Cooperative bacterial-mediated proteolysis of MUC5B was determined by comparing individual and mixed consortia of strains isolated from supragingival plaque and freshly harvested supragingival plaque. Proteolytic activity was analyzed using fluorescent labelled substrate and by visualizing mucin degradation by SDS-PAGE. Dental plaque degraded the polypeptide backbone of the salivary MUC5B mucin. The mucin also was degraded by a specific consortium of isolated species from supragingival plaque, although individual species and other consortia did not. Select bacteria in supragingival dental plaque, therefore, cooperate as a consortium to proteolyze human salivary MUC5B and hydrolyze glycosides.}},
author = {{Wickström, Claes and Herzberg, Mark C and Beighton, David and Svensater, Gunnel}},
issn = {{1465-2080}},
language = {{eng}},
month = {{06}},
publisher = {{MAIK Nauka/Interperiodica}},
series = {{Microbiology}},
title = {{Proteolytic degradation of human salivary MUC5B by dental biofilms.}},
url = {{http://dx.doi.org/10.1099/mic.0.030536-0}},
doi = {{10.1099/mic.0.030536-0}},
year = {{2009}},
}