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Proteolytic degradation of human salivary MUC5B by dental biofilms.

Wickström, Claes LU ; Herzberg, Mark C; Beighton, David and Svensater, Gunnel (2009) In Microbiology1994-01-01+01:00
Abstract
The degradation of complex substrates, like salivary mucins, requires an arsenal of glycosidases and proteases to sequentially degrade the oligosaccharides and polypeptide backbone. MUC5B is a complex oligomeric glycoprotein, heterogeneous in molecular mass (14-40 x 106 Da), with a diverse repertoire of oligosaccharides, differing in composition and charge. The aim of this study was to investigate whether proteolytic degradation of the mucin polypeptide backbone could be identified and if cooperation of dental biofilm bacteria was required. Cooperative bacterial-mediated proteolysis of MUC5B was determined by comparing individual and mixed consortia of strains isolated from supragingival plaque and freshly harvested supragingival plaque.... (More)
The degradation of complex substrates, like salivary mucins, requires an arsenal of glycosidases and proteases to sequentially degrade the oligosaccharides and polypeptide backbone. MUC5B is a complex oligomeric glycoprotein, heterogeneous in molecular mass (14-40 x 106 Da), with a diverse repertoire of oligosaccharides, differing in composition and charge. The aim of this study was to investigate whether proteolytic degradation of the mucin polypeptide backbone could be identified and if cooperation of dental biofilm bacteria was required. Cooperative bacterial-mediated proteolysis of MUC5B was determined by comparing individual and mixed consortia of strains isolated from supragingival plaque and freshly harvested supragingival plaque. Proteolytic activity was analyzed using fluorescent labelled substrate and by visualizing mucin degradation by SDS-PAGE. Dental plaque degraded the polypeptide backbone of the salivary MUC5B mucin. The mucin also was degraded by a specific consortium of isolated species from supragingival plaque, although individual species and other consortia did not. Select bacteria in supragingival dental plaque, therefore, cooperate as a consortium to proteolyze human salivary MUC5B and hydrolyze glycosides. (Less)
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author
organization
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Contribution to journal
publication status
published
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in
Microbiology1994-01-01+01:00
publisher
MAIK Nauka/Interperiodica
external identifiers
  • pmid:19556293
  • scopus:69949129819
ISSN
1465-2080
DOI
10.1099/mic.0.030536-0
language
English
LU publication?
yes
id
f2dfe253-2d45-4b6a-b0ed-be1f730a644c (old id 1433878)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19556293?dopt=Abstract
date added to LUP
2009-07-06 11:14:00
date last changed
2017-10-01 04:55:11
@article{f2dfe253-2d45-4b6a-b0ed-be1f730a644c,
  abstract     = {The degradation of complex substrates, like salivary mucins, requires an arsenal of glycosidases and proteases to sequentially degrade the oligosaccharides and polypeptide backbone. MUC5B is a complex oligomeric glycoprotein, heterogeneous in molecular mass (14-40 x 106 Da), with a diverse repertoire of oligosaccharides, differing in composition and charge. The aim of this study was to investigate whether proteolytic degradation of the mucin polypeptide backbone could be identified and if cooperation of dental biofilm bacteria was required. Cooperative bacterial-mediated proteolysis of MUC5B was determined by comparing individual and mixed consortia of strains isolated from supragingival plaque and freshly harvested supragingival plaque. Proteolytic activity was analyzed using fluorescent labelled substrate and by visualizing mucin degradation by SDS-PAGE. Dental plaque degraded the polypeptide backbone of the salivary MUC5B mucin. The mucin also was degraded by a specific consortium of isolated species from supragingival plaque, although individual species and other consortia did not. Select bacteria in supragingival dental plaque, therefore, cooperate as a consortium to proteolyze human salivary MUC5B and hydrolyze glycosides.},
  author       = {Wickström, Claes and Herzberg, Mark C and Beighton, David and Svensater, Gunnel},
  issn         = {1465-2080},
  language     = {eng},
  month        = {06},
  publisher    = {MAIK Nauka/Interperiodica},
  series       = {Microbiology1994-01-01+01:00},
  title        = {Proteolytic degradation of human salivary MUC5B by dental biofilms.},
  url          = {http://dx.doi.org/10.1099/mic.0.030536-0},
  year         = {2009},
}