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The utilization of glycogen accumulating organisms for mixed culture production of polyhydroxyalkanoates.

Bengtsson, Simon LU (2009) In Biotechnology and Bioengineering 104. p.698-708
Abstract
Production of polyhydroxyalkanoates (PHA) by an open mixed culture enriched in glycogen accumulating organisms (GAOs) under alternating anaerobic-aerobic conditions with acetate as carbon source was investigated. The culture exhibited a stable enrichment performance over the 450 day operating period with regards to phenotypic behavior and microbial community structure. Candidatus Competibacter phosphatis dominated the culture at between 54 and 70 % of the bacterial biomass throughout the study, as determined by fluorescence in situ hybridization. In batch experiments under anaerobic conditions, PHA containing 3-hydroxybutyrate (3HB) and 27 mol-% 3-hydroxyvalerate (3HV) was accumulated up to 49 % of cell dry weight utilizing the glycogen... (More)
Production of polyhydroxyalkanoates (PHA) by an open mixed culture enriched in glycogen accumulating organisms (GAOs) under alternating anaerobic-aerobic conditions with acetate as carbon source was investigated. The culture exhibited a stable enrichment performance over the 450 day operating period with regards to phenotypic behavior and microbial community structure. Candidatus Competibacter phosphatis dominated the culture at between 54 and 70 % of the bacterial biomass throughout the study, as determined by fluorescence in situ hybridization. In batch experiments under anaerobic conditions, PHA containing 3-hydroxybutyrate (3HB) and 27 mol-% 3-hydroxyvalerate (3HV) was accumulated up to 49 % of cell dry weight utilizing the glycogen pool stored in the SBR cycle. Under aerobic and ammonia limited conditions, PHA comprising only 3HB was accumulated to 60 % of cell dry weight. Glycogen was consumed during aerobic PHA accumulation as well as under anaerobic conditions, but with different stoichiometry. Under aerobic conditions 0.31 C-mol glycogen was consumed per consumed C-mol acetate compared to 0.99 under anaerobic conditions. Both the PHA biomass content and the specific PHA production rate obtained were similar to what is typically obtained using the more commonly applied aerobic dynamic feeding strategy. (c) 2009 Wiley Periodicals, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology and Bioengineering
volume
104
pages
698 - 708
publisher
John Wiley & Sons
external identifiers
  • wos:000271173400007
  • pmid:19530079
  • scopus:70350492881
ISSN
1097-0290
DOI
10.1002/bit.22444
language
English
LU publication?
yes
id
ec2c1a2d-860b-4670-8e37-a1347916c97d (old id 1434243)
date added to LUP
2009-07-02 14:59:09
date last changed
2017-03-26 03:40:57
@article{ec2c1a2d-860b-4670-8e37-a1347916c97d,
  abstract     = {Production of polyhydroxyalkanoates (PHA) by an open mixed culture enriched in glycogen accumulating organisms (GAOs) under alternating anaerobic-aerobic conditions with acetate as carbon source was investigated. The culture exhibited a stable enrichment performance over the 450 day operating period with regards to phenotypic behavior and microbial community structure. Candidatus Competibacter phosphatis dominated the culture at between 54 and 70 % of the bacterial biomass throughout the study, as determined by fluorescence in situ hybridization. In batch experiments under anaerobic conditions, PHA containing 3-hydroxybutyrate (3HB) and 27 mol-% 3-hydroxyvalerate (3HV) was accumulated up to 49 % of cell dry weight utilizing the glycogen pool stored in the SBR cycle. Under aerobic and ammonia limited conditions, PHA comprising only 3HB was accumulated to 60 % of cell dry weight. Glycogen was consumed during aerobic PHA accumulation as well as under anaerobic conditions, but with different stoichiometry. Under aerobic conditions 0.31 C-mol glycogen was consumed per consumed C-mol acetate compared to 0.99 under anaerobic conditions. Both the PHA biomass content and the specific PHA production rate obtained were similar to what is typically obtained using the more commonly applied aerobic dynamic feeding strategy. (c) 2009 Wiley Periodicals, Inc.},
  author       = {Bengtsson, Simon},
  issn         = {1097-0290},
  language     = {eng},
  pages        = {698--708},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {The utilization of glycogen accumulating organisms for mixed culture production of polyhydroxyalkanoates.},
  url          = {http://dx.doi.org/10.1002/bit.22444},
  volume       = {104},
  year         = {2009},
}