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Monitoring of glucose and glutamate using enzyme microstructures and scanning electrochemical microscopy.

Muresan, Laura LU ; Nistor, Mihaela LU ; Gáspár, Szilveszter ; Popescu, Ionel Cătălin and Csöregi, Elisabeth LU (2009) In Bioelectrochemistry 76. p.81-86
Abstract
Glucose oxidase and glutamate oxidase lines, with typical width of 100 microm, were patterned on gold surfaces using a micro-dispensing system, by shooting 100 pl droplets of the corresponding enzyme solutions. The probe of a scanning electrochemical microscope (SECM) was then carefully positioned in the close proximity of the enzyme microstructure and poised to +600 mV vs. Ag/AgCl, KCl 0.1 M. The H(2)O(2), generated by the enzyme lines at different concentrations of glucose and glutamate in the surrounding solution, was sequentially monitored. Reproducible calibration curves for glucose and glutamate were obtained in one single experiment, proving that the combination of enzyme microstructures with SECM can provide a new way of achieving... (More)
Glucose oxidase and glutamate oxidase lines, with typical width of 100 microm, were patterned on gold surfaces using a micro-dispensing system, by shooting 100 pl droplets of the corresponding enzyme solutions. The probe of a scanning electrochemical microscope (SECM) was then carefully positioned in the close proximity of the enzyme microstructure and poised to +600 mV vs. Ag/AgCl, KCl 0.1 M. The H(2)O(2), generated by the enzyme lines at different concentrations of glucose and glutamate in the surrounding solution, was sequentially monitored. Reproducible calibration curves for glucose and glutamate were obtained in one single experiment, proving that the combination of enzyme microstructures with SECM can provide a new way of achieving multianalyte detection. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Bioelectrochemistry
volume
76
pages
81 - 86
publisher
Elsevier
external identifiers
  • wos:000270014400014
  • pmid:19520620
  • scopus:68549094259
  • pmid:19520620
ISSN
1878-562X
DOI
10.1016/j.bioelechem.2009.05.004
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
id
9e45df28-9c96-4b05-bb5f-533acbd174c6 (old id 1434296)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19520620?dopt=Abstract
date added to LUP
2016-04-01 12:25:30
date last changed
2021-02-17 04:15:28
@article{9e45df28-9c96-4b05-bb5f-533acbd174c6,
  abstract     = {Glucose oxidase and glutamate oxidase lines, with typical width of 100 microm, were patterned on gold surfaces using a micro-dispensing system, by shooting 100 pl droplets of the corresponding enzyme solutions. The probe of a scanning electrochemical microscope (SECM) was then carefully positioned in the close proximity of the enzyme microstructure and poised to +600 mV vs. Ag/AgCl, KCl 0.1 M. The H(2)O(2), generated by the enzyme lines at different concentrations of glucose and glutamate in the surrounding solution, was sequentially monitored. Reproducible calibration curves for glucose and glutamate were obtained in one single experiment, proving that the combination of enzyme microstructures with SECM can provide a new way of achieving multianalyte detection.},
  author       = {Muresan, Laura and Nistor, Mihaela and Gáspár, Szilveszter and Popescu, Ionel Cătălin and Csöregi, Elisabeth},
  issn         = {1878-562X},
  language     = {eng},
  pages        = {81--86},
  publisher    = {Elsevier},
  series       = {Bioelectrochemistry},
  title        = {Monitoring of glucose and glutamate using enzyme microstructures and scanning electrochemical microscopy.},
  url          = {http://dx.doi.org/10.1016/j.bioelechem.2009.05.004},
  doi          = {10.1016/j.bioelechem.2009.05.004},
  volume       = {76},
  year         = {2009},
}