Pooling control in variable preparative chromatography processes.
Westerberg, Karin LU ; Degerman, Marcus LU and Nilsson, Bernt LU
(2010)
In Bioprocess and Biosystems Engineering
33.
p.375-382
- Abstract
- Preparative chromatographic columns that run at high loads are highly sensitive to batch-to-batch disturbances of the process parameters, placing high demands on the strategy used for pooling of the product fractions. A new approach to pooling control is presented in a proof-of-concept study. A model-based sensitivity analysis was performed identifying the critical process parameters to product purity and optimal cut points. From this, the robust fixed cut points were found and pooling control strategies for variations in the critical parameters were designed. Direct measurements and indirect measurements based on the UV detector signal were used as control signals. The method is demonstrated for two case studies of preparative protein... (More)
- Preparative chromatographic columns that run at high loads are highly sensitive to batch-to-batch disturbances of the process parameters, placing high demands on the strategy used for pooling of the product fractions. A new approach to pooling control is presented in a proof-of-concept study. A model-based sensitivity analysis was performed identifying the critical process parameters to product purity and optimal cut points. From this, the robust fixed cut points were found and pooling control strategies for variations in the critical parameters were designed. Direct measurements and indirect measurements based on the UV detector signal were used as control signals. The method is demonstrated for two case studies of preparative protein chromatography: hydrophobic interaction and reversed phase chromatography. The yield improved from 88.18 to 92.88% when changing from fixed to variable pooling in hydrophobic interaction chromatography, and from 35.15 to 76.27% in the highly sensitive reversed phase chromatography. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1434351
- author
- Westerberg, Karin
LU
; Degerman, Marcus
LU
and Nilsson, Bernt
LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Bioprocess and Biosystems Engineering
- volume
- 33
- pages
- 375 - 382
- publisher
- Springer
- external identifiers
-
- wos:000274398800010
- pmid:19513758
- scopus:77649236686
- ISSN
- 1615-7605
- DOI
- 10.1007/s00449-009-0335-8
- language
- English
- LU publication?
- yes
- id
- a8113e4a-e601-4a0f-afe4-33b19890915f (old id 1434351)
- date added to LUP
- 2016-04-01 14:09:29
- date last changed
- 2024-10-10 12:47:18
@article{a8113e4a-e601-4a0f-afe4-33b19890915f,
abstract = {{Preparative chromatographic columns that run at high loads are highly sensitive to batch-to-batch disturbances of the process parameters, placing high demands on the strategy used for pooling of the product fractions. A new approach to pooling control is presented in a proof-of-concept study. A model-based sensitivity analysis was performed identifying the critical process parameters to product purity and optimal cut points. From this, the robust fixed cut points were found and pooling control strategies for variations in the critical parameters were designed. Direct measurements and indirect measurements based on the UV detector signal were used as control signals. The method is demonstrated for two case studies of preparative protein chromatography: hydrophobic interaction and reversed phase chromatography. The yield improved from 88.18 to 92.88% when changing from fixed to variable pooling in hydrophobic interaction chromatography, and from 35.15 to 76.27% in the highly sensitive reversed phase chromatography.}},
author = {{Westerberg, Karin and Degerman, Marcus and Nilsson, Bernt}},
issn = {{1615-7605}},
language = {{eng}},
pages = {{375--382}},
publisher = {{Springer}},
series = {{Bioprocess and Biosystems Engineering}},
title = {{Pooling control in variable preparative chromatography processes.}},
url = {{http://dx.doi.org/10.1007/s00449-009-0335-8}},
doi = {{10.1007/s00449-009-0335-8}},
volume = {{33}},
year = {{2010}},
}