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Subcellular localization, modification and protein complex formation of the cdk-inhibitor p16 in Rb-functional and Rb-inactivated tumor cells.

Nilsson, Kristina LU and Landberg, Göran LU (2006) In International Journal of Cancer 118(5). p.1120-1125
Abstract
The cdk-inhibitor p16 is a tumor suppressor gene that is inactivated in many forms of cancer. Despite numerous studies, the exact mechanism of regulation of p16 has not been clarified, although the status of retinoblastoma (Rb) seems to be one important factor that influences the p16 expression. The specificity and validity of cytoplasmic localization of p16 observed in some tumors has further been questioned. Here, by subcellular fractionation of Rb-functional and Rb-inactivated cell lines, we show that p16 indeed is expressed in the cytoplasm as well as in the nucleus. Post translational modifications of p16 in different subcellular compartments as well as its capacity to form protein complexes were further delineated. Two dimensional... (More)
The cdk-inhibitor p16 is a tumor suppressor gene that is inactivated in many forms of cancer. Despite numerous studies, the exact mechanism of regulation of p16 has not been clarified, although the status of retinoblastoma (Rb) seems to be one important factor that influences the p16 expression. The specificity and validity of cytoplasmic localization of p16 observed in some tumors has further been questioned. Here, by subcellular fractionation of Rb-functional and Rb-inactivated cell lines, we show that p16 indeed is expressed in the cytoplasm as well as in the nucleus. Post translational modifications of p16 in different subcellular compartments as well as its capacity to form protein complexes were further delineated. Two dimensional gel electrophoresis showed that two forms of p16 appeared in the cytoplasm, while only one form was detected in the nucleus. Samples of basal cell carcinoma and squamous cell carcinoma of the skin with either functional or non-functional Rb also exhibited at least two forms of p16. In addition, cytoplasmic p16 bound cyclin dependent kinase (cdk)4/6, potentially indicating that p16 could have a function in the cytoplasm. (c) 2005 Wiley-Liss, Inc. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
two-dimensional gel electrophoresis, retinoblastoma gene product, p16
in
International Journal of Cancer
volume
118
issue
5
pages
1120 - 1125
publisher
John Wiley and Sons
external identifiers
  • pmid:16161044
  • wos:000235056100006
  • scopus:31844446633
  • pmid:16161044
ISSN
0020-7136
DOI
10.1002/ijc.21466
language
English
LU publication?
yes
id
13706c18-35e8-4a68-9b35-206cebae07c9 (old id 143677)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16161044&dopt=Abstract
date added to LUP
2016-04-01 12:09:40
date last changed
2021-10-03 04:40:39
@article{13706c18-35e8-4a68-9b35-206cebae07c9,
  abstract     = {The cdk-inhibitor p16 is a tumor suppressor gene that is inactivated in many forms of cancer. Despite numerous studies, the exact mechanism of regulation of p16 has not been clarified, although the status of retinoblastoma (Rb) seems to be one important factor that influences the p16 expression. The specificity and validity of cytoplasmic localization of p16 observed in some tumors has further been questioned. Here, by subcellular fractionation of Rb-functional and Rb-inactivated cell lines, we show that p16 indeed is expressed in the cytoplasm as well as in the nucleus. Post translational modifications of p16 in different subcellular compartments as well as its capacity to form protein complexes were further delineated. Two dimensional gel electrophoresis showed that two forms of p16 appeared in the cytoplasm, while only one form was detected in the nucleus. Samples of basal cell carcinoma and squamous cell carcinoma of the skin with either functional or non-functional Rb also exhibited at least two forms of p16. In addition, cytoplasmic p16 bound cyclin dependent kinase (cdk)4/6, potentially indicating that p16 could have a function in the cytoplasm. (c) 2005 Wiley-Liss, Inc.},
  author       = {Nilsson, Kristina and Landberg, Göran},
  issn         = {0020-7136},
  language     = {eng},
  number       = {5},
  pages        = {1120--1125},
  publisher    = {John Wiley and Sons},
  series       = {International Journal of Cancer},
  title        = {Subcellular localization, modification and protein complex formation of the cdk-inhibitor p16 in Rb-functional and Rb-inactivated tumor cells.},
  url          = {http://dx.doi.org/10.1002/ijc.21466},
  doi          = {10.1002/ijc.21466},
  volume       = {118},
  year         = {2006},
}