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Selective nucleotide-release from dense-core granules in insulin-secreting cells.

Obermüller, Stefanie LU ; Lindqvist, Anders LU ; Karanauskaite, Jovita LU ; Galvanovskis, Juris; Rorsman, Patrik LU and Barg, Sebastian LU (2005) In Journal of Cell Science 118(Pt 18). p.4271-4282
Abstract
Secretory granules of insulin-secreting cells are used to store and release peptide hormones as well as low-molecular-weight compounds such as nucleotides. Here we have compared the rate of exocytosis with the time courses of nucleotide and peptide release by a combination of capacitance measurements, electrophysiological detection of ATP release and single-granule imaging. We demonstrate that the release of nucleotides and peptides is delayed by similar to 0.1 and similar to 2 seconds with respect to membrane fusion, respectively. We further show that in up to 70% of the cases exocytosis does not result in significant release of the peptide cargo, likely because of a mechanism that leads to premature closure of the fusion pore. Release of... (More)
Secretory granules of insulin-secreting cells are used to store and release peptide hormones as well as low-molecular-weight compounds such as nucleotides. Here we have compared the rate of exocytosis with the time courses of nucleotide and peptide release by a combination of capacitance measurements, electrophysiological detection of ATP release and single-granule imaging. We demonstrate that the release of nucleotides and peptides is delayed by similar to 0.1 and similar to 2 seconds with respect to membrane fusion, respectively. We further show that in up to 70% of the cases exocytosis does not result in significant release of the peptide cargo, likely because of a mechanism that leads to premature closure of the fusion pore. Release of nucleotides and protons occurred regardless of whether peptides were secreted or not. These observations suggest that insulin-secreting cells are able to use the same secretory vesicles to release small molecules either alone or together with the peptide hormone. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
insulin, hormone, fusion pore, exocytosis, endocytosis, kiss-and-run, secretion
in
Journal of Cell Science
volume
118
issue
Pt 18
pages
4271 - 4282
publisher
The Company of Biologists Ltd
external identifiers
  • wos:000232549000020
  • pmid:16141231
  • scopus:27144434554
ISSN
0021-9533
DOI
10.1242/jcs.02549
language
English
LU publication?
yes
id
ff77c11a-e395-46b9-8c7b-1bdeba8bef94 (old id 143866)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16141231&dopt=Abstract
date added to LUP
2007-07-18 13:48:48
date last changed
2017-11-12 03:26:19
@article{ff77c11a-e395-46b9-8c7b-1bdeba8bef94,
  abstract     = {Secretory granules of insulin-secreting cells are used to store and release peptide hormones as well as low-molecular-weight compounds such as nucleotides. Here we have compared the rate of exocytosis with the time courses of nucleotide and peptide release by a combination of capacitance measurements, electrophysiological detection of ATP release and single-granule imaging. We demonstrate that the release of nucleotides and peptides is delayed by similar to 0.1 and similar to 2 seconds with respect to membrane fusion, respectively. We further show that in up to 70% of the cases exocytosis does not result in significant release of the peptide cargo, likely because of a mechanism that leads to premature closure of the fusion pore. Release of nucleotides and protons occurred regardless of whether peptides were secreted or not. These observations suggest that insulin-secreting cells are able to use the same secretory vesicles to release small molecules either alone or together with the peptide hormone.},
  author       = {Obermüller, Stefanie and Lindqvist, Anders and Karanauskaite, Jovita and Galvanovskis, Juris and Rorsman, Patrik and Barg, Sebastian},
  issn         = {0021-9533},
  keyword      = {insulin,hormone,fusion pore,exocytosis,endocytosis,kiss-and-run,secretion},
  language     = {eng},
  number       = {Pt 18},
  pages        = {4271--4282},
  publisher    = {The Company of Biologists Ltd},
  series       = {Journal of Cell Science},
  title        = {Selective nucleotide-release from dense-core granules in insulin-secreting cells.},
  url          = {http://dx.doi.org/10.1242/jcs.02549},
  volume       = {118},
  year         = {2005},
}