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Laminin {alpha}1 Chain Corrects Male Infertility Caused by Absence of Laminin {alpha}2 Chain.

Häger, Mattias LU ; Gawlik, Kinga LU ; Nyström, Alexander LU ; Sasaki, Takako and Durbeej-Hjalt, Madeleine LU (2005) In American Journal of Pathology 167(3). p.823-833
Abstract
Laminins are important for basement membrane structure and function. The laminin alpha2 chain is a major component of muscle basement membranes, and mutations in the laminin alpha2 gene lead to congenital muscular dystrophy in humans and mice. Although the laminin alpha2 chain is prominently expressed in testicular basement membranes, its role in testis has remained unclear. Here, we show that laminin alpha1, alpha2, beta1, beta2, gamma 1, and gamma 3 chains are the major laminin chains in basement membranes of seminiferous tubules. In laminin alpha2 chain-deficient dy(3 K)/dy(3 ASK) mice, lack of laminin alpha2 chain led to concurrent reduction of laminin gamma 3 chain and abnormal testicular basement membranes. Seminiferous tubules of... (More)
Laminins are important for basement membrane structure and function. The laminin alpha2 chain is a major component of muscle basement membranes, and mutations in the laminin alpha2 gene lead to congenital muscular dystrophy in humans and mice. Although the laminin alpha2 chain is prominently expressed in testicular basement membranes, its role in testis has remained unclear. Here, we show that laminin alpha1, alpha2, beta1, beta2, gamma 1, and gamma 3 chains are the major laminin chains in basement membranes of seminiferous tubules. In laminin alpha2 chain-deficient dy(3 K)/dy(3 ASK) mice, lack of laminin alpha2 chain led to concurrent reduction of laminin gamma 3 chain and abnormal testicular basement membranes. Seminiferous tubules of laminin alpha2 chain-deficient dy(3 K)/dy(3 K) mice displayed a defect in the timing of lumen formation, resulting in production of fewer spermatides. We also demonstrate that overexpression of laminin alpha1 chain in testis of dy(3 K)/dy(3 K) mice compensated for laminin alpha2 chain deficiency and significantly reversed the appearance of the histopathological features. We thus provide genetic data that laminin alpha chains are essential for normal testicular function in vivo. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
American Journal of Pathology
volume
167
issue
3
pages
823 - 833
publisher
American Society for Investigative Pathology
external identifiers
  • wos:000231514500017
  • pmid:16127160
ISSN
1525-2191
language
English
LU publication?
yes
id
e936512c-ce57-4724-836e-43a29647cb87 (old id 144017)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16127160&dopt=Abstract
date added to LUP
2007-07-13 14:11:00
date last changed
2016-04-15 20:28:48
@article{e936512c-ce57-4724-836e-43a29647cb87,
  abstract     = {Laminins are important for basement membrane structure and function. The laminin alpha2 chain is a major component of muscle basement membranes, and mutations in the laminin alpha2 gene lead to congenital muscular dystrophy in humans and mice. Although the laminin alpha2 chain is prominently expressed in testicular basement membranes, its role in testis has remained unclear. Here, we show that laminin alpha1, alpha2, beta1, beta2, gamma 1, and gamma 3 chains are the major laminin chains in basement membranes of seminiferous tubules. In laminin alpha2 chain-deficient dy(3 K)/dy(3 ASK) mice, lack of laminin alpha2 chain led to concurrent reduction of laminin gamma 3 chain and abnormal testicular basement membranes. Seminiferous tubules of laminin alpha2 chain-deficient dy(3 K)/dy(3 K) mice displayed a defect in the timing of lumen formation, resulting in production of fewer spermatides. We also demonstrate that overexpression of laminin alpha1 chain in testis of dy(3 K)/dy(3 K) mice compensated for laminin alpha2 chain deficiency and significantly reversed the appearance of the histopathological features. We thus provide genetic data that laminin alpha chains are essential for normal testicular function in vivo.},
  author       = {Häger, Mattias and Gawlik, Kinga and Nyström, Alexander and Sasaki, Takako and Durbeej-Hjalt, Madeleine},
  issn         = {1525-2191},
  language     = {eng},
  number       = {3},
  pages        = {823--833},
  publisher    = {American Society for Investigative Pathology},
  series       = {American Journal of Pathology},
  title        = {Laminin {alpha}1 Chain Corrects Male Infertility Caused by Absence of Laminin {alpha}2 Chain.},
  volume       = {167},
  year         = {2005},
}