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DNA gel nanoparticles: preparation and controlling the size

Moran, M. C.; Baptista, F. R.; Ramalho, A.; Miguel, M. G. and Lindman, Björn LU (2009) In Soft Matter 5(13). p.2538-2542
Abstract
A simple and novel method was used for the preparation of nano-/micro-sized DNA gel particles by nebulisation of a solution of DNA (single-or double-stranded) into an oppositely charged surfactant (cetyltrimethylammonium bromide) or protein (lysozyme, protamine sulfate) solution. The size and size distribution of the particle populations were investigated by means of fluorescence microscopy (FM), photon correlation spectroscopy (PCS) and scanning electron microscopy (SEM). Particles measuring from 0.1 to 10 mu m were obtained. FM studies suggest that the formation of the particles was carried out with conservation of the secondary structure of the nucleic acid molecules. SEM on freeze-dried and Au-shadowed samples showed a distribution of... (More)
A simple and novel method was used for the preparation of nano-/micro-sized DNA gel particles by nebulisation of a solution of DNA (single-or double-stranded) into an oppositely charged surfactant (cetyltrimethylammonium bromide) or protein (lysozyme, protamine sulfate) solution. The size and size distribution of the particle populations were investigated by means of fluorescence microscopy (FM), photon correlation spectroscopy (PCS) and scanning electron microscopy (SEM). Particles measuring from 0.1 to 10 mu m were obtained. FM studies suggest that the formation of the particles was carried out with conservation of the secondary structure of the nucleic acid molecules. SEM on freeze-dried and Au-shadowed samples showed a distribution of virtually spherical particles. It was found that, in addition to the size of the initial DNA droplets, the cationic agent is a controlling parameter of the particle size. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Soft Matter
volume
5
issue
13
pages
2538 - 2542
publisher
Royal Society of Chemistry
external identifiers
  • wos:000267272100005
  • scopus:70349275278
ISSN
1744-6848
DOI
10.1039/b822169c
language
English
LU publication?
yes
id
a00915c6-403f-4b9b-9ec7-b3a1012b7a71 (old id 1440557)
date added to LUP
2009-07-28 12:35:40
date last changed
2017-08-27 04:42:38
@article{a00915c6-403f-4b9b-9ec7-b3a1012b7a71,
  abstract     = {A simple and novel method was used for the preparation of nano-/micro-sized DNA gel particles by nebulisation of a solution of DNA (single-or double-stranded) into an oppositely charged surfactant (cetyltrimethylammonium bromide) or protein (lysozyme, protamine sulfate) solution. The size and size distribution of the particle populations were investigated by means of fluorescence microscopy (FM), photon correlation spectroscopy (PCS) and scanning electron microscopy (SEM). Particles measuring from 0.1 to 10 mu m were obtained. FM studies suggest that the formation of the particles was carried out with conservation of the secondary structure of the nucleic acid molecules. SEM on freeze-dried and Au-shadowed samples showed a distribution of virtually spherical particles. It was found that, in addition to the size of the initial DNA droplets, the cationic agent is a controlling parameter of the particle size.},
  author       = {Moran, M. C. and Baptista, F. R. and Ramalho, A. and Miguel, M. G. and Lindman, Björn},
  issn         = {1744-6848},
  language     = {eng},
  number       = {13},
  pages        = {2538--2542},
  publisher    = {Royal Society of Chemistry},
  series       = {Soft Matter},
  title        = {DNA gel nanoparticles: preparation and controlling the size},
  url          = {http://dx.doi.org/10.1039/b822169c},
  volume       = {5},
  year         = {2009},
}