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Is the variability of nickel patch test reactivity over time associated with fluctuations in the systemic T-cell reactivity to nickel?

Masjedi, K.; Bruze, Magnus LU ; Hindsén, Monica LU ; Minang, J. and Ahlborg, N. (2009) In British Journal of Dermatology 161(1). p.102-109
Abstract
Background Patch test reactivity to nickel varies over time. To what extent this variation is associated with fluctuations in the T-cell reactivity to nickel is not known. Objectives Our aim was to investigate the relationship between variation over time in the patch test and the systemic T-cell reactivity to nickel. Methods Patients (n = 15) with a history of contact allergy to nickel were subjected to three consecutive patch tests at 3-month intervals, utilizing NiSO4 at 10 concentrations ranging from 0.0032% to 12.5%. Prior to each patch test, blood mononuclear cells were analysed for T-cell reactivity to nickel by interleukin (IL)-4 and IL-13 enzyme-linked immunospot assay. Results Eleven patients reacted positively in all three patch... (More)
Background Patch test reactivity to nickel varies over time. To what extent this variation is associated with fluctuations in the T-cell reactivity to nickel is not known. Objectives Our aim was to investigate the relationship between variation over time in the patch test and the systemic T-cell reactivity to nickel. Methods Patients (n = 15) with a history of contact allergy to nickel were subjected to three consecutive patch tests at 3-month intervals, utilizing NiSO4 at 10 concentrations ranging from 0.0032% to 12.5%. Prior to each patch test, blood mononuclear cells were analysed for T-cell reactivity to nickel by interleukin (IL)-4 and IL-13 enzyme-linked immunospot assay. Results Eleven patients reacted positively in all three patch tests, two patients reacted in one or two tests and two remained negative. All 13 positive patients displayed variability over time, in terms of the lowest dose of nickel to which they responded. Also the cytokine response to nickel varied over time but the patients' mean cytokine response was positively correlated with their mean patch test reactivity (r(s) = 0.70, P < 0.01 for IL-4; r(s) = 0.78, P < 0.001 for IL-13). However, although the changes over time in patch test reactivity and the cytokine responses to nickel displayed a similar pattern in many patients, there was no significant correlation between the individuals' variation over time in vivo and in vitro. Conclusions The overall magnitude of the T-cell reactivity to nickel and the patch test reactivity are closely associated but fluctuations in the systemic T-cell reactivity cannot be singled out as the major cause of longitudinal variability in nickel patch test reactivity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cytokine, contact allergy, patch test, enzyme-linked immunospot assay
in
British Journal of Dermatology
volume
161
issue
1
pages
102 - 109
publisher
Wiley-Blackwell
external identifiers
  • wos:000267128500014
  • scopus:67649986295
ISSN
1365-2133
DOI
10.1111/j.1365-2133.2009.09182.x
language
English
LU publication?
yes
id
6f601f45-7f5c-4dd5-a14f-90196922f89b (old id 1441710)
date added to LUP
2009-07-27 15:27:56
date last changed
2017-11-05 03:30:38
@article{6f601f45-7f5c-4dd5-a14f-90196922f89b,
  abstract     = {Background Patch test reactivity to nickel varies over time. To what extent this variation is associated with fluctuations in the T-cell reactivity to nickel is not known. Objectives Our aim was to investigate the relationship between variation over time in the patch test and the systemic T-cell reactivity to nickel. Methods Patients (n = 15) with a history of contact allergy to nickel were subjected to three consecutive patch tests at 3-month intervals, utilizing NiSO4 at 10 concentrations ranging from 0.0032% to 12.5%. Prior to each patch test, blood mononuclear cells were analysed for T-cell reactivity to nickel by interleukin (IL)-4 and IL-13 enzyme-linked immunospot assay. Results Eleven patients reacted positively in all three patch tests, two patients reacted in one or two tests and two remained negative. All 13 positive patients displayed variability over time, in terms of the lowest dose of nickel to which they responded. Also the cytokine response to nickel varied over time but the patients' mean cytokine response was positively correlated with their mean patch test reactivity (r(s) = 0.70, P &lt; 0.01 for IL-4; r(s) = 0.78, P &lt; 0.001 for IL-13). However, although the changes over time in patch test reactivity and the cytokine responses to nickel displayed a similar pattern in many patients, there was no significant correlation between the individuals' variation over time in vivo and in vitro. Conclusions The overall magnitude of the T-cell reactivity to nickel and the patch test reactivity are closely associated but fluctuations in the systemic T-cell reactivity cannot be singled out as the major cause of longitudinal variability in nickel patch test reactivity.},
  author       = {Masjedi, K. and Bruze, Magnus and Hindsén, Monica and Minang, J. and Ahlborg, N.},
  issn         = {1365-2133},
  keyword      = {cytokine,contact allergy,patch test,enzyme-linked immunospot assay},
  language     = {eng},
  number       = {1},
  pages        = {102--109},
  publisher    = {Wiley-Blackwell},
  series       = {British Journal of Dermatology},
  title        = {Is the variability of nickel patch test reactivity over time associated with fluctuations in the systemic T-cell reactivity to nickel?},
  url          = {http://dx.doi.org/10.1111/j.1365-2133.2009.09182.x},
  volume       = {161},
  year         = {2009},
}