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Detection of Bronchial Function of NHBD Lung Following One-h Warm Ischemia by Organ Bath Model

Yang, Yang; Zhao, Song; Liao, Qiuming LU and Wang, Jianjun (2009) In Journal of Huazhong University of Science and Technology-Medical Sciences 29(3). p.340-343
Abstract
This study investigated the feasibility and effects of organ bath to be used for detection of bronchial function of non-heart-beating donor (NHBD) lung after 1-h warm ischemia. Sixteen Swedish pigs were divided into two groups randomly: heart-beating donor (HBD) group and NHBD with 1-h warm ischemia (NHBD-1 h) group. The bronchial rings whose lengths and inner diameters were both 1.5 mm were obtained from isolated left lungs of all the pigs. Acetylcholine, arachidonic acid natrium and papaverine were used to test and compare the contractile and relaxant function of bronchial smooth muscles and epithelium-dependent relaxation (EpiDR) response between HBD and NHBD-1 h groups. The results showed that there was no significant difference in the... (More)
This study investigated the feasibility and effects of organ bath to be used for detection of bronchial function of non-heart-beating donor (NHBD) lung after 1-h warm ischemia. Sixteen Swedish pigs were divided into two groups randomly: heart-beating donor (HBD) group and NHBD with 1-h warm ischemia (NHBD-1 h) group. The bronchial rings whose lengths and inner diameters were both 1.5 mm were obtained from isolated left lungs of all the pigs. Acetylcholine, arachidonic acid natrium and papaverine were used to test and compare the contractile and relaxant function of bronchial smooth muscles and epithelium-dependent relaxation (EpiDR) response between HBD and NHBD-1 h groups. The results showed that there was no significant difference in the values of bronchial precontraction between HBD and NHBD-1 h groups (5.18 +/- 0.07 vs 5.10 +/- 0.11 mN, P > 0.05). No significant difference in the values of EpiDR responses between HBD and NHBD-1 h groups (1.26 +/- 0.05 vs 1.23 +/- 0.07 mN, P > 0.05) was observed either. During the process of EpiDR induction, the rings had no spontaneous relaxation in two groups. In addition, papaverine solution completely relaxed the bronchial smooth muscles of all bronchial rings. It was concluded that after warm ischemia for 1 h, the contractile and relaxant abilities of bronchial smooth muscles, and the epithelium-dependent adjustment both kept intact. Organ bath model could be a liable and scientific way to evaluate the bronchial function of NHBD lung. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
non-heart-beating donor, organ bath, epithelium-dependent relaxation
in
Journal of Huazhong University of Science and Technology-Medical Sciences
volume
29
issue
3
pages
340 - 343
publisher
Huazhong University of Science and Technology
external identifiers
  • wos:000266911500015
  • scopus:70349655550
ISSN
1993-1352
DOI
10.1007/s11596-009-0315-x
language
English
LU publication?
yes
id
34f7da55-ce65-4509-b5f4-7d686e4a53a8 (old id 1442049)
date added to LUP
2009-07-27 13:33:04
date last changed
2017-01-01 04:44:00
@article{34f7da55-ce65-4509-b5f4-7d686e4a53a8,
  abstract     = {This study investigated the feasibility and effects of organ bath to be used for detection of bronchial function of non-heart-beating donor (NHBD) lung after 1-h warm ischemia. Sixteen Swedish pigs were divided into two groups randomly: heart-beating donor (HBD) group and NHBD with 1-h warm ischemia (NHBD-1 h) group. The bronchial rings whose lengths and inner diameters were both 1.5 mm were obtained from isolated left lungs of all the pigs. Acetylcholine, arachidonic acid natrium and papaverine were used to test and compare the contractile and relaxant function of bronchial smooth muscles and epithelium-dependent relaxation (EpiDR) response between HBD and NHBD-1 h groups. The results showed that there was no significant difference in the values of bronchial precontraction between HBD and NHBD-1 h groups (5.18 +/- 0.07 vs 5.10 +/- 0.11 mN, P > 0.05). No significant difference in the values of EpiDR responses between HBD and NHBD-1 h groups (1.26 +/- 0.05 vs 1.23 +/- 0.07 mN, P > 0.05) was observed either. During the process of EpiDR induction, the rings had no spontaneous relaxation in two groups. In addition, papaverine solution completely relaxed the bronchial smooth muscles of all bronchial rings. It was concluded that after warm ischemia for 1 h, the contractile and relaxant abilities of bronchial smooth muscles, and the epithelium-dependent adjustment both kept intact. Organ bath model could be a liable and scientific way to evaluate the bronchial function of NHBD lung.},
  author       = {Yang, Yang and Zhao, Song and Liao, Qiuming and Wang, Jianjun},
  issn         = {1993-1352},
  keyword      = {non-heart-beating donor,organ bath,epithelium-dependent relaxation},
  language     = {eng},
  number       = {3},
  pages        = {340--343},
  publisher    = {Huazhong University of Science and Technology},
  series       = {Journal of Huazhong University of Science and Technology-Medical Sciences},
  title        = {Detection of Bronchial Function of NHBD Lung Following One-h Warm Ischemia by Organ Bath Model},
  url          = {http://dx.doi.org/10.1007/s11596-009-0315-x},
  volume       = {29},
  year         = {2009},
}