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Studies on toll-like receptor stimuli responsiveness in HIV-1 and HIV-2 infections

Nowroozalizadeh, Salma; Månsson, Fredrik LU ; da Silva, Zacarias; Repits, Johanna LU ; Dabo, Braima; Pereira, Carla; Biague, Antonio; Albert, Jan; Nielsen, Jens and Aaby, Peter, et al. (2009) In Cytokine 46(3). p.325-331
Abstract
Background: HIV-1 and HIV-2 are two related viruses with distinct clinical outcomes, where HIV-1 is more pathogenic and transmissible than HIV-2. The pathogenesis of both infections is influenced by the dysregulation and deterioration of the adaptive immune system. However, their effects on the responsiveness of innate immunity are less well known. Here, we report on toll-like receptor (TLR) stimuli responsiveness in HIV-1 or HIV-2 infections. Methods: Whole blood from 235 individuals living in Guinea-Bissau who were uninfected, infected with HIV-1, infected with HIV-2, and/or infected with HTLV-1, was stimulated with TLR7/8 and TLR9 agonists, R-848 and unmethylated CpG DNA. After TLR7/8 and TLR9 stimuli, the expression levels of IL-12 and... (More)
Background: HIV-1 and HIV-2 are two related viruses with distinct clinical outcomes, where HIV-1 is more pathogenic and transmissible than HIV-2. The pathogenesis of both infections is influenced by the dysregulation and deterioration of the adaptive immune system. However, their effects on the responsiveness of innate immunity are less well known. Here, we report on toll-like receptor (TLR) stimuli responsiveness in HIV-1 or HIV-2 infections. Methods: Whole blood from 235 individuals living in Guinea-Bissau who were uninfected, infected with HIV-1, infected with HIV-2, and/or infected with HTLV-1, was stimulated with TLR7/8 and TLR9 agonists, R-848 and unmethylated CpG DNA. After TLR7/8 and TLR9 stimuli, the expression levels of IL-12 and IFN-alpha were related to gender, age, infection status, CD4(+) T cell counts. and plasma viral load. Results: Defective TLR9 responsiveness was observed in the advanced disease stage, along with CD4(+) T cell loss in both HIV-1 and HIV-2 infections. Moreover, TLR7/8 responsiveness was reduced in HIV-1 infected individuals compared with uninfected controls. Conclusions: Innate immunity responsiveness can be monitored by whole blood stimulation. Both advanced HIVA and HIV-2 infections may cause innate immunity dysregulation. (C) 2009 Elsevier Ltd. All rights reserved. (Less)
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published
subject
keywords
HIV-1, HIV-2, Toll-like receptors, IL-12, IFN-alpha
in
Cytokine
volume
46
issue
3
pages
325 - 331
publisher
Academic Press
external identifiers
  • wos:000266962900006
  • scopus:67349216345
ISSN
1096-0023
DOI
10.1016/j.cyto.2009.03.003
language
English
LU publication?
yes
id
c63ef6a7-b34f-431b-8ae7-104d2d99da1a (old id 1442290)
date added to LUP
2009-07-27 11:02:00
date last changed
2017-01-01 06:05:03
@article{c63ef6a7-b34f-431b-8ae7-104d2d99da1a,
  abstract     = {Background: HIV-1 and HIV-2 are two related viruses with distinct clinical outcomes, where HIV-1 is more pathogenic and transmissible than HIV-2. The pathogenesis of both infections is influenced by the dysregulation and deterioration of the adaptive immune system. However, their effects on the responsiveness of innate immunity are less well known. Here, we report on toll-like receptor (TLR) stimuli responsiveness in HIV-1 or HIV-2 infections. Methods: Whole blood from 235 individuals living in Guinea-Bissau who were uninfected, infected with HIV-1, infected with HIV-2, and/or infected with HTLV-1, was stimulated with TLR7/8 and TLR9 agonists, R-848 and unmethylated CpG DNA. After TLR7/8 and TLR9 stimuli, the expression levels of IL-12 and IFN-alpha were related to gender, age, infection status, CD4(+) T cell counts. and plasma viral load. Results: Defective TLR9 responsiveness was observed in the advanced disease stage, along with CD4(+) T cell loss in both HIV-1 and HIV-2 infections. Moreover, TLR7/8 responsiveness was reduced in HIV-1 infected individuals compared with uninfected controls. Conclusions: Innate immunity responsiveness can be monitored by whole blood stimulation. Both advanced HIVA and HIV-2 infections may cause innate immunity dysregulation. (C) 2009 Elsevier Ltd. All rights reserved.},
  author       = {Nowroozalizadeh, Salma and Månsson, Fredrik and da Silva, Zacarias and Repits, Johanna and Dabo, Braima and Pereira, Carla and Biague, Antonio and Albert, Jan and Nielsen, Jens and Aaby, Peter and Fenyö, Eva Maria and Norrgren, Hans and Holmgren, Birgitta G and Jansson, Marianne},
  issn         = {1096-0023},
  keyword      = {HIV-1,HIV-2,Toll-like receptors,IL-12,IFN-alpha},
  language     = {eng},
  number       = {3},
  pages        = {325--331},
  publisher    = {Academic Press},
  series       = {Cytokine},
  title        = {Studies on toll-like receptor stimuli responsiveness in HIV-1 and HIV-2 infections},
  url          = {http://dx.doi.org/10.1016/j.cyto.2009.03.003},
  volume       = {46},
  year         = {2009},
}