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Compact oleic acid in HAMLET.

Fast, Jonas LU ; Mossberg, Anki LU ; Nilsson, Hanna LU ; Svanborg, Catharina LU ; Akke, Mikael LU and Linse, Sara LU (2005) In FEBS Letters 579(27). p.6095-6100
Abstract
HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a complex between α-lactalbumin and oleic acid that induces apoptosis in tumor cells, but not in healthy cells. Heteronuclear nuclear magnetic resonance (NMR) spectroscopy was used to determine the structure of 13C-oleic acid in HAMLET, and to study the 15N-labeled protein. Nuclear Overhauser enhancement spectroscopy shows that the two ends of the fatty acid are in close proximity and close to the double bond, indicating that the oleic acid is bound to HAMLET in a compact conformation. The data further show that HAMLET is a partly unfolded/molten globule-like complex under physiological conditions.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
FEBS Letters
volume
579
issue
27
pages
6095 - 6100
publisher
Wiley-Blackwell
external identifiers
  • wos:000233157800017
  • pmid:16229842
  • scopus:27544442240
ISSN
1873-3468
DOI
10.1016/j.febslet.2005.08.089
language
English
LU publication?
yes
id
7fd767ac-a16f-49f1-ba64-de600d1290de (old id 144601)
date added to LUP
2007-06-29 09:46:44
date last changed
2017-07-30 04:30:21
@article{7fd767ac-a16f-49f1-ba64-de600d1290de,
  abstract     = {HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a complex between α-lactalbumin and oleic acid that induces apoptosis in tumor cells, but not in healthy cells. Heteronuclear nuclear magnetic resonance (NMR) spectroscopy was used to determine the structure of 13C-oleic acid in HAMLET, and to study the 15N-labeled protein. Nuclear Overhauser enhancement spectroscopy shows that the two ends of the fatty acid are in close proximity and close to the double bond, indicating that the oleic acid is bound to HAMLET in a compact conformation. The data further show that HAMLET is a partly unfolded/molten globule-like complex under physiological conditions.},
  author       = {Fast, Jonas and Mossberg, Anki and Nilsson, Hanna and Svanborg, Catharina and Akke, Mikael and Linse, Sara},
  issn         = {1873-3468},
  language     = {eng},
  number       = {27},
  pages        = {6095--6100},
  publisher    = {Wiley-Blackwell},
  series       = {FEBS Letters},
  title        = {Compact oleic acid in HAMLET.},
  url          = {http://dx.doi.org/10.1016/j.febslet.2005.08.089},
  volume       = {579},
  year         = {2005},
}