Compact oleic acid in HAMLET.
Fast, Jonas LU ; Mossberg, Anki LU ; Nilsson, Hanna LU ; Svanborg, Catharina LU ; Akke, Mikael LU
and Linse, Sara
LU
(2005)
In FEBS Letters
579(27).
p.6095-6100
- Abstract
- HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a complex between α-lactalbumin and oleic acid that induces apoptosis in tumor cells, but not in healthy cells. Heteronuclear nuclear magnetic resonance (NMR) spectroscopy was used to determine the structure of 13C-oleic acid in HAMLET, and to study the 15N-labeled protein. Nuclear Overhauser enhancement spectroscopy shows that the two ends of the fatty acid are in close proximity and close to the double bond, indicating that the oleic acid is bound to HAMLET in a compact conformation. The data further show that HAMLET is a partly unfolded/molten globule-like complex under physiological conditions.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/144601
- author
- Fast, Jonas
LU
; Mossberg, Anki
LU
; Nilsson, Hanna
LU
; Svanborg, Catharina
LU
; Akke, Mikael
LU
and Linse, Sara
LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- FEBS Letters
- volume
- 579
- issue
- 27
- pages
- 6095 - 6100
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000233157800017
- pmid:16229842
- scopus:27544442240
- ISSN
- 1873-3468
- DOI
- 10.1016/j.febslet.2005.08.089
- language
- English
- LU publication?
- yes
- id
- 7fd767ac-a16f-49f1-ba64-de600d1290de (old id 144601)
- date added to LUP
- 2016-04-01 15:52:06
- date last changed
- 2022-01-28 07:38:47
@article{7fd767ac-a16f-49f1-ba64-de600d1290de,
abstract = {{HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a complex between α-lactalbumin and oleic acid that induces apoptosis in tumor cells, but not in healthy cells. Heteronuclear nuclear magnetic resonance (NMR) spectroscopy was used to determine the structure of 13C-oleic acid in HAMLET, and to study the 15N-labeled protein. Nuclear Overhauser enhancement spectroscopy shows that the two ends of the fatty acid are in close proximity and close to the double bond, indicating that the oleic acid is bound to HAMLET in a compact conformation. The data further show that HAMLET is a partly unfolded/molten globule-like complex under physiological conditions.}},
author = {{Fast, Jonas and Mossberg, Anki and Nilsson, Hanna and Svanborg, Catharina and Akke, Mikael and Linse, Sara}},
issn = {{1873-3468}},
language = {{eng}},
number = {{27}},
pages = {{6095--6100}},
publisher = {{Wiley-Blackwell}},
series = {{FEBS Letters}},
title = {{Compact oleic acid in HAMLET.}},
url = {{https://lup.lub.lu.se/search/files/4497484/625010.pdf}},
doi = {{10.1016/j.febslet.2005.08.089}},
volume = {{579}},
year = {{2005}},
}