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HpaA is essential for Helicobacter pylori colonization in mice

Carlsohn, Elisabet; Nyström, Johanna; Bölin, Ingrid; Nilsson, Carol L LU and Svennerholm, Ann-Mari (2006) In Infection and Immunity 74(2). p.6-920
Abstract

Infection with the human gastric pathogen Helicobacter pylori can give rise to chronic gastritis, peptic ulcer, and gastric cancer. All H. pylori strains express the surface-localized protein HpaA, a promising candidate for a vaccine against H. pylori infection. To study the physiological importance of HpaA, a mutation of the hpaA gene was introduced into a mouse-adapted H. pylori strain. To justify that the interruption of the hpaA gene did not cause any polar effects of downstream genes or was associated with a second site mutation, the protein expression patterns of the mutant and wild-type strains were characterized by two different proteomic approaches. Two-dimensional differential in-gel electrophoresis analysis of whole-cell... (More)

Infection with the human gastric pathogen Helicobacter pylori can give rise to chronic gastritis, peptic ulcer, and gastric cancer. All H. pylori strains express the surface-localized protein HpaA, a promising candidate for a vaccine against H. pylori infection. To study the physiological importance of HpaA, a mutation of the hpaA gene was introduced into a mouse-adapted H. pylori strain. To justify that the interruption of the hpaA gene did not cause any polar effects of downstream genes or was associated with a second site mutation, the protein expression patterns of the mutant and wild-type strains were characterized by two different proteomic approaches. Two-dimensional differential in-gel electrophoresis analysis of whole-cell extracts and subcellular fractionation combined with nano-liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometry for outer membrane protein profiling revealed only minor differences in the protein profile between the mutant and the wild-type strains. Therefore, the mutant strain was tested for its colonizing ability in a well-established mouse model. While inoculation with the wild-type strain resulted in heavily H. pylori-infected mice, the HpaA mutant strain was not able to establish colonization. Thus, by combining proteomic analysis and in vivo studies, we conclude that HpaA is essential for the colonization of H. pylori in mice.

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Contribution to journal
publication status
published
keywords
Adhesins, Bacterial, Animals, Bacterial Proteins, Female, Gastric Mucosa, Gene Expression Profiling, Gene Expression Regulation, Bacterial, Helicobacter Infections, Helicobacter pylori, Humans, Mice, Mice, Inbred C57BL, Mutation, Proteome, Journal Article, Research Support, Non-U.S. Gov't
in
Infection and Immunity
volume
74
issue
2
pages
7 pages
publisher
American Society for Microbiology
external identifiers
  • scopus:31844455162
ISSN
0019-9567
DOI
10.1128/IAI.74.2.920-926.2006
language
English
LU publication?
no
id
14520c35-1b19-4ce3-a571-b273c8ab15ef
date added to LUP
2017-05-16 10:35:31
date last changed
2018-10-14 04:39:30
@article{14520c35-1b19-4ce3-a571-b273c8ab15ef,
  abstract     = {<p>Infection with the human gastric pathogen Helicobacter pylori can give rise to chronic gastritis, peptic ulcer, and gastric cancer. All H. pylori strains express the surface-localized protein HpaA, a promising candidate for a vaccine against H. pylori infection. To study the physiological importance of HpaA, a mutation of the hpaA gene was introduced into a mouse-adapted H. pylori strain. To justify that the interruption of the hpaA gene did not cause any polar effects of downstream genes or was associated with a second site mutation, the protein expression patterns of the mutant and wild-type strains were characterized by two different proteomic approaches. Two-dimensional differential in-gel electrophoresis analysis of whole-cell extracts and subcellular fractionation combined with nano-liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometry for outer membrane protein profiling revealed only minor differences in the protein profile between the mutant and the wild-type strains. Therefore, the mutant strain was tested for its colonizing ability in a well-established mouse model. While inoculation with the wild-type strain resulted in heavily H. pylori-infected mice, the HpaA mutant strain was not able to establish colonization. Thus, by combining proteomic analysis and in vivo studies, we conclude that HpaA is essential for the colonization of H. pylori in mice.</p>},
  author       = {Carlsohn, Elisabet and Nyström, Johanna and Bölin, Ingrid and Nilsson, Carol L and Svennerholm, Ann-Mari},
  issn         = {0019-9567},
  keyword      = {Adhesins, Bacterial,Animals,Bacterial Proteins,Female,Gastric Mucosa,Gene Expression Profiling,Gene Expression Regulation, Bacterial,Helicobacter Infections,Helicobacter pylori,Humans,Mice,Mice, Inbred C57BL,Mutation,Proteome,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {2},
  pages        = {6--920},
  publisher    = {American Society for Microbiology},
  series       = {Infection and Immunity},
  title        = {HpaA is essential for Helicobacter pylori colonization in mice},
  url          = {http://dx.doi.org/10.1128/IAI.74.2.920-926.2006},
  volume       = {74},
  year         = {2006},
}