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Development and validation of LC-MS/MS method for the determination of cyproheptadine in several pharmaceutical syrup formulations.

Feás, Xesús; Ye, Lei LU ; Hosseini, Seyed V; Fente, Cristina A and Cepeda, Alberto (2009) In Journal of Pharmaceutical and Biomedical Analysis 50. p.1044-1049
Abstract
A rapid and sensitive liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the qualitative and quantitative assay of cyproheptadine (CP) in pharmaceutical samples. Diphenylpyraline hydrochloride (DPP) was used as an internal standard (IS). Two multiple reaction-monitoring (MRM) transitions for each analyte were observed: 288.1/96.1 and 288.1/191.2 for CP and 282.1/167.2 and 282.1/116.3 for DPP. The retention time of the drug was 7.29min. The analytical method was successfully validated for linearity (1-100ng/ml), intra-day precision, inter-day precision, and accuracy. The limit of detection (LOD) and limit of quantification (LOQ) were 0.86 and 0.98ng/ml, respectively. The proposed method was... (More)
A rapid and sensitive liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the qualitative and quantitative assay of cyproheptadine (CP) in pharmaceutical samples. Diphenylpyraline hydrochloride (DPP) was used as an internal standard (IS). Two multiple reaction-monitoring (MRM) transitions for each analyte were observed: 288.1/96.1 and 288.1/191.2 for CP and 282.1/167.2 and 282.1/116.3 for DPP. The retention time of the drug was 7.29min. The analytical method was successfully validated for linearity (1-100ng/ml), intra-day precision, inter-day precision, and accuracy. The limit of detection (LOD) and limit of quantification (LOQ) were 0.86 and 0.98ng/ml, respectively. The proposed method was applied to analyse the cyproheptadine content from seven different syrup formulations. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Pharmaceutical and Biomedical Analysis
volume
50
pages
1044 - 1049
publisher
Elsevier
external identifiers
  • wos:000270759600054
  • pmid:19574011
  • scopus:70349189156
ISSN
0731-7085
DOI
10.1016/j.jpba.2009.06.006
language
English
LU publication?
yes
id
d27f7ab4-05d9-4727-a3de-86f8164a5ba1 (old id 1453442)
date added to LUP
2009-09-01 09:16:39
date last changed
2017-01-01 04:58:17
@article{d27f7ab4-05d9-4727-a3de-86f8164a5ba1,
  abstract     = {A rapid and sensitive liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the qualitative and quantitative assay of cyproheptadine (CP) in pharmaceutical samples. Diphenylpyraline hydrochloride (DPP) was used as an internal standard (IS). Two multiple reaction-monitoring (MRM) transitions for each analyte were observed: 288.1/96.1 and 288.1/191.2 for CP and 282.1/167.2 and 282.1/116.3 for DPP. The retention time of the drug was 7.29min. The analytical method was successfully validated for linearity (1-100ng/ml), intra-day precision, inter-day precision, and accuracy. The limit of detection (LOD) and limit of quantification (LOQ) were 0.86 and 0.98ng/ml, respectively. The proposed method was applied to analyse the cyproheptadine content from seven different syrup formulations.},
  author       = {Feás, Xesús and Ye, Lei and Hosseini, Seyed V and Fente, Cristina A and Cepeda, Alberto},
  issn         = {0731-7085},
  language     = {eng},
  pages        = {1044--1049},
  publisher    = {Elsevier},
  series       = {Journal of Pharmaceutical and Biomedical Analysis},
  title        = {Development and validation of LC-MS/MS method for the determination of cyproheptadine in several pharmaceutical syrup formulations.},
  url          = {http://dx.doi.org/10.1016/j.jpba.2009.06.006},
  volume       = {50},
  year         = {2009},
}