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Label free capacitive immunosensor for detecting calpastatin - A meat tenderness biomarker.

Zor, Kinga LU ; Ortiz, Roberto LU ; Saatci, Ebru; Bardsley, Ronald; Parr, Tim; Csöregi, Elisabeth LU and Nistor, Mihaela LU (2009) In Bioelectrochemistry 76(1-2). p.93-99
Abstract
An immunological capacitive biosensor for calpastatin was developed, optimized and applied for the analysis of meat extract samples. Anti-calpastatin antibody was immobilized on a gold electrode modified with a self-assembled monolayer of mercaptoundecanoic acid and Protein A from Staphylococcus aureus, and the obtained immunosensor was inserted as the working electrode in an electrochemical cell of a flow injection system. The dynamic range of the sensor was 20 to 160 ng/mL calpastatin. The electrode could be regenerated and re-used for more than 7 days with minimal reduction in sensitivity. For the analysis of real samples, the target analyte was extracted from the Longissimus dorsi muscle from beef carcasses directly after slaughtering.... (More)
An immunological capacitive biosensor for calpastatin was developed, optimized and applied for the analysis of meat extract samples. Anti-calpastatin antibody was immobilized on a gold electrode modified with a self-assembled monolayer of mercaptoundecanoic acid and Protein A from Staphylococcus aureus, and the obtained immunosensor was inserted as the working electrode in an electrochemical cell of a flow injection system. The dynamic range of the sensor was 20 to 160 ng/mL calpastatin. The electrode could be regenerated and re-used for more than 7 days with minimal reduction in sensitivity. For the analysis of real samples, the target analyte was extracted from the Longissimus dorsi muscle from beef carcasses directly after slaughtering. The extract was analyzed both with the developed immunosensor and microtiter plate ELISA, and a good correlation was obtained. However the immunosensor offers advantages of speed, simplicity, sensitivity and possibility for miniaturization over conventional assays for calpastatin quantification. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Bioelectrochemistry
volume
76
issue
1-2
pages
93 - 99
publisher
Elsevier
external identifiers
  • wos:000270014400016
  • pmid:19564137
  • scopus:68549110181
ISSN
1878-562X
DOI
10.1016/j.bioelechem.2009.06.001
language
English
LU publication?
yes
id
f32fc252-0b63-4638-9dc1-dac07de4cc51 (old id 1453516)
date added to LUP
2009-09-01 09:13:56
date last changed
2017-08-27 04:15:46
@article{f32fc252-0b63-4638-9dc1-dac07de4cc51,
  abstract     = {An immunological capacitive biosensor for calpastatin was developed, optimized and applied for the analysis of meat extract samples. Anti-calpastatin antibody was immobilized on a gold electrode modified with a self-assembled monolayer of mercaptoundecanoic acid and Protein A from Staphylococcus aureus, and the obtained immunosensor was inserted as the working electrode in an electrochemical cell of a flow injection system. The dynamic range of the sensor was 20 to 160 ng/mL calpastatin. The electrode could be regenerated and re-used for more than 7 days with minimal reduction in sensitivity. For the analysis of real samples, the target analyte was extracted from the Longissimus dorsi muscle from beef carcasses directly after slaughtering. The extract was analyzed both with the developed immunosensor and microtiter plate ELISA, and a good correlation was obtained. However the immunosensor offers advantages of speed, simplicity, sensitivity and possibility for miniaturization over conventional assays for calpastatin quantification.},
  author       = {Zor, Kinga and Ortiz, Roberto and Saatci, Ebru and Bardsley, Ronald and Parr, Tim and Csöregi, Elisabeth and Nistor, Mihaela},
  issn         = {1878-562X},
  language     = {eng},
  number       = {1-2},
  pages        = {93--99},
  publisher    = {Elsevier},
  series       = {Bioelectrochemistry},
  title        = {Label free capacitive immunosensor for detecting calpastatin - A meat tenderness biomarker.},
  url          = {http://dx.doi.org/10.1016/j.bioelechem.2009.06.001},
  volume       = {76},
  year         = {2009},
}