Advanced

Automated microfluidic chromatin immunoprecipitation from 2,000 cells

Wu, Angela R.; Hiatt, Joseph B.; Lu, Rung; Attema, Joanne LU ; Lobo, Neethan A.; Weissman, Irving L.; Clarke, Michael F. and Quake, Stephen R. (2009) In Lab on A Chip 9(10). p.1365-1370
Abstract
Chromatin immunoprecipitation (ChIP) is a powerful assay used to probe DNA-protein interactions. Traditional methods of implementing this assay are lengthy, Cumbersome and require a large number of cells, making it difficult to study rare cell types Such its certain cancer and stem cells. We have designed a microfluidic device to perform sensitive ChIP analysis on low cell numbers in a rapid, automated fashion while preserving the specificity of the assay. Comparing ChIP results for two modified histone protein targets, we showed our automated microfluidic ChIP (AutoChIP) from 2,000 cells to be comparable to that of conventional ChIP methods using 50,000-500,000 cells. This technology may provide a solution to the need for a high... (More)
Chromatin immunoprecipitation (ChIP) is a powerful assay used to probe DNA-protein interactions. Traditional methods of implementing this assay are lengthy, Cumbersome and require a large number of cells, making it difficult to study rare cell types Such its certain cancer and stem cells. We have designed a microfluidic device to perform sensitive ChIP analysis on low cell numbers in a rapid, automated fashion while preserving the specificity of the assay. Comparing ChIP results for two modified histone protein targets, we showed our automated microfluidic ChIP (AutoChIP) from 2,000 cells to be comparable to that of conventional ChIP methods using 50,000-500,000 cells. This technology may provide a solution to the need for a high sensitivity, rapid, and automated ChIP assay. and in doing so facilitate the use of ChIP for many interesting and valuable applications. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lab on A Chip
volume
9
issue
10
pages
1365 - 1370
publisher
Royal Society of Chemistry
external identifiers
  • wos:000268227400008
  • scopus:65649130015
ISSN
1473-0189
DOI
10.1039/b819648f
language
English
LU publication?
yes
id
229e83db-1c7d-4e88-9c2c-5860232d5504 (old id 1460495)
date added to LUP
2009-08-31 11:07:07
date last changed
2017-12-10 03:40:28
@article{229e83db-1c7d-4e88-9c2c-5860232d5504,
  abstract     = {Chromatin immunoprecipitation (ChIP) is a powerful assay used to probe DNA-protein interactions. Traditional methods of implementing this assay are lengthy, Cumbersome and require a large number of cells, making it difficult to study rare cell types Such its certain cancer and stem cells. We have designed a microfluidic device to perform sensitive ChIP analysis on low cell numbers in a rapid, automated fashion while preserving the specificity of the assay. Comparing ChIP results for two modified histone protein targets, we showed our automated microfluidic ChIP (AutoChIP) from 2,000 cells to be comparable to that of conventional ChIP methods using 50,000-500,000 cells. This technology may provide a solution to the need for a high sensitivity, rapid, and automated ChIP assay. and in doing so facilitate the use of ChIP for many interesting and valuable applications.},
  author       = {Wu, Angela R. and Hiatt, Joseph B. and Lu, Rung and Attema, Joanne and Lobo, Neethan A. and Weissman, Irving L. and Clarke, Michael F. and Quake, Stephen R.},
  issn         = {1473-0189},
  language     = {eng},
  number       = {10},
  pages        = {1365--1370},
  publisher    = {Royal Society of Chemistry},
  series       = {Lab on A Chip},
  title        = {Automated microfluidic chromatin immunoprecipitation from 2,000 cells},
  url          = {http://dx.doi.org/10.1039/b819648f},
  volume       = {9},
  year         = {2009},
}