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The use of ScCO2 for the extraction of LPS from S. enterica subsp. PCM 2266

Rybka, J. ; Grycko, P. ; da Cruz Francisco, José LU ; Gamian, A. and Dey, Estera LU (2009) 2nd International Conference on Environmental, Industrial and Applied Microbiology p.460-464
Abstract
The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266). Process parameters for the extraction of LPS from bacterial mass were optimized by carrying out a two-level fractional design experiment. Four parameters, namely temperature, CO, flow rate, pressure and co-solvent composition were analyzed. The best crude extract yields were achieved when the CO, flow rate and temperature were kept high (10g/min, 90 degrees C) and pure water was used as a co-solvent. Pressure had no statistically significant effects within the range of the study performed, whereas the other factors were relevant. The recovery of the extracted LPS by scCO(2) was about 3.3% of the biomass used,... (More)
The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266). Process parameters for the extraction of LPS from bacterial mass were optimized by carrying out a two-level fractional design experiment. Four parameters, namely temperature, CO, flow rate, pressure and co-solvent composition were analyzed. The best crude extract yields were achieved when the CO, flow rate and temperature were kept high (10g/min, 90 degrees C) and pure water was used as a co-solvent. Pressure had no statistically significant effects within the range of the study performed, whereas the other factors were relevant. The recovery of the extracted LPS by scCO(2) was about 3.3% of the biomass used, while in the classical extraction method yield was less than 2%. All isolates were characterized by SDS-PAGE, by the spectra of the thiobarbituric acid reaction products and GLC-MS analysis. (Less)
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author
; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
vaccine, Salmonella, lipopolysaccharides (LPS), Supercritical carbon dioxide (scCO(2))
host publication
Current Research Topics In Applied Microbiology And Microbial Biotechnology
pages
460 - 464
publisher
World Scientific Publishing
conference name
2nd International Conference on Environmental, Industrial and Applied Microbiology
conference location
Seville, Spain
conference dates
2007-11-28 - 2007-12-01
external identifiers
  • wos:000268100200096
language
English
LU publication?
yes
id
1b897095-0346-4727-a980-52584d0a7aac (old id 1461867)
date added to LUP
2016-04-04 11:57:19
date last changed
2023-04-18 18:21:38
@inproceedings{1b897095-0346-4727-a980-52584d0a7aac,
  abstract     = {{The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266). Process parameters for the extraction of LPS from bacterial mass were optimized by carrying out a two-level fractional design experiment. Four parameters, namely temperature, CO, flow rate, pressure and co-solvent composition were analyzed. The best crude extract yields were achieved when the CO, flow rate and temperature were kept high (10g/min, 90 degrees C) and pure water was used as a co-solvent. Pressure had no statistically significant effects within the range of the study performed, whereas the other factors were relevant. The recovery of the extracted LPS by scCO(2) was about 3.3% of the biomass used, while in the classical extraction method yield was less than 2%. All isolates were characterized by SDS-PAGE, by the spectra of the thiobarbituric acid reaction products and GLC-MS analysis.}},
  author       = {{Rybka, J. and Grycko, P. and da Cruz Francisco, José and Gamian, A. and Dey, Estera}},
  booktitle    = {{Current Research Topics In Applied Microbiology And Microbial Biotechnology}},
  keywords     = {{vaccine; Salmonella; lipopolysaccharides (LPS); Supercritical carbon dioxide (scCO(2))}},
  language     = {{eng}},
  pages        = {{460--464}},
  publisher    = {{World Scientific Publishing}},
  title        = {{The use of ScCO2 for the extraction of LPS from S. enterica subsp. PCM 2266}},
  year         = {{2009}},
}