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Fate of N-15 labelled nitrate and ammonium in a fertilized forest soil

Bengtsson, Göran LU and Bergwall, C (2000) In Soil Biology & Biochemistry 32(4). p.545-557
Abstract
The possibility that long-term atmospheric nitrogen pollution and fertilization of forest soil may serve as a basis for adaptation for enhanced transformation rates of NO3- and NH4+ in soil. bacteria was elucidated in a laboratory bioassay. Bacteria extracted from soils that had been fertilized at various rates for the last 30 yr were characterised with respect to their capability to reduce or oxidise different nitrogen sources. The same soils were used under oxic or anoxic conditions to quantify denitrification, dissimilatory nitrate reduction to ammonium (DNRA) and nitrification. (NO3-)-N-15 and (NH4+)-N-15 were added as tracers to the soils, which were incubated in bottles for 3 to 5 d. Concentrations of (N2O)-N-15 in headspace and... (More)
The possibility that long-term atmospheric nitrogen pollution and fertilization of forest soil may serve as a basis for adaptation for enhanced transformation rates of NO3- and NH4+ in soil. bacteria was elucidated in a laboratory bioassay. Bacteria extracted from soils that had been fertilized at various rates for the last 30 yr were characterised with respect to their capability to reduce or oxidise different nitrogen sources. The same soils were used under oxic or anoxic conditions to quantify denitrification, dissimilatory nitrate reduction to ammonium (DNRA) and nitrification. (NO3-)-N-15 and (NH4+)-N-15 were added as tracers to the soils, which were incubated in bottles for 3 to 5 d. Concentrations of (N2O)-N-15 in headspace and (NO3-)-N-15 and (NH4+)-N-15 in soil extracts were determined by gas chromatography-mass spectrometry. Total numbers of bacteria were similar in all soils and ranged from 3 to 4 x 10(8) cells g(-1) d.wt. of soil. Between 50 and 70% of the isolated strains were capable of reducing nitrate and the majority of them reduced nitrate to ammonium. About 0.01 parts per thousand of all isolates were classified as nitrifiers. Both nitrate reducers and nitrifiers were more common in fertilized soils than in the unfertilized control soil. The foremost fate of added (NO3-)-N-15 and (NH4+)-N-15 in all soils was immobilisation. More than 85% was immobilised in anoxic soils and between 64 and 97% in the oxic soils, with the lowest quantities in fertilized soils. As regards the remaining, non-immobilised N, DNRA dominated over denitrification, as could be expected from the higher frequency of ammonifying bacteria compared with denitrifiers. There was no obvious relationship between NH4+ produced and the amount of fertilizer applied, whereas denitrification was negatively correlated with the amount of fertilizer applied. Nitrifying activity was low in all soils with no obvious relationship between NO3- produced and fertilizer applied. Hence, no correlation was found between the relative abundance of N transforming bacteria and the transformation activity. The N flux followed essentially the same pattern as that seen for product formation. The DNRA Aux was higher than that of both denitrification and nitrification. DNRA and denitrification fluxes were highest in the control soil, whereas the nitrification flux was low in all soils. The absence of evidence for adaptation to enhanced rates of transformation of NO3- and NH4+ in soil bacteria exposed to long-term N fertilization is reflected by the low concentration of extractable inorganic N in the fertilized soils. As a consequence of the quantitative importance of immobilization of added N, differences in physiological capacity evolved in soil bacteria to immobilize and mobilise N may determine the rates by which inorganic N is available for plant growth or lost to groundwater and air. (C) 2000 Elsevier Science Ltd. All rights reserved. (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
in
Soil Biology & Biochemistry
volume
32
issue
4
pages
545 - 557
publisher
Elsevier
external identifiers
  • scopus:0034070472
ISSN
0038-0717
DOI
10.1016/S0038-0717(99)00183-2
language
English
LU publication?
yes
id
0c658a75-1fae-4f28-ad45-068690e9a819 (old id 146243)
date added to LUP
2007-06-27 08:13:01
date last changed
2017-08-06 04:29:59
@article{0c658a75-1fae-4f28-ad45-068690e9a819,
  abstract     = {The possibility that long-term atmospheric nitrogen pollution and fertilization of forest soil may serve as a basis for adaptation for enhanced transformation rates of NO3- and NH4+ in soil. bacteria was elucidated in a laboratory bioassay. Bacteria extracted from soils that had been fertilized at various rates for the last 30 yr were characterised with respect to their capability to reduce or oxidise different nitrogen sources. The same soils were used under oxic or anoxic conditions to quantify denitrification, dissimilatory nitrate reduction to ammonium (DNRA) and nitrification. (NO3-)-N-15 and (NH4+)-N-15 were added as tracers to the soils, which were incubated in bottles for 3 to 5 d. Concentrations of (N2O)-N-15 in headspace and (NO3-)-N-15 and (NH4+)-N-15 in soil extracts were determined by gas chromatography-mass spectrometry. Total numbers of bacteria were similar in all soils and ranged from 3 to 4 x 10(8) cells g(-1) d.wt. of soil. Between 50 and 70% of the isolated strains were capable of reducing nitrate and the majority of them reduced nitrate to ammonium. About 0.01 parts per thousand of all isolates were classified as nitrifiers. Both nitrate reducers and nitrifiers were more common in fertilized soils than in the unfertilized control soil. The foremost fate of added (NO3-)-N-15 and (NH4+)-N-15 in all soils was immobilisation. More than 85% was immobilised in anoxic soils and between 64 and 97% in the oxic soils, with the lowest quantities in fertilized soils. As regards the remaining, non-immobilised N, DNRA dominated over denitrification, as could be expected from the higher frequency of ammonifying bacteria compared with denitrifiers. There was no obvious relationship between NH4+ produced and the amount of fertilizer applied, whereas denitrification was negatively correlated with the amount of fertilizer applied. Nitrifying activity was low in all soils with no obvious relationship between NO3- produced and fertilizer applied. Hence, no correlation was found between the relative abundance of N transforming bacteria and the transformation activity. The N flux followed essentially the same pattern as that seen for product formation. The DNRA Aux was higher than that of both denitrification and nitrification. DNRA and denitrification fluxes were highest in the control soil, whereas the nitrification flux was low in all soils. The absence of evidence for adaptation to enhanced rates of transformation of NO3- and NH4+ in soil bacteria exposed to long-term N fertilization is reflected by the low concentration of extractable inorganic N in the fertilized soils. As a consequence of the quantitative importance of immobilization of added N, differences in physiological capacity evolved in soil bacteria to immobilize and mobilise N may determine the rates by which inorganic N is available for plant growth or lost to groundwater and air. (C) 2000 Elsevier Science Ltd. All rights reserved.},
  author       = {Bengtsson, Göran and Bergwall, C},
  issn         = {0038-0717},
  language     = {eng},
  number       = {4},
  pages        = {545--557},
  publisher    = {Elsevier},
  series       = {Soil Biology & Biochemistry},
  title        = {Fate of N-15 labelled nitrate and ammonium in a fertilized forest soil},
  url          = {http://dx.doi.org/10.1016/S0038-0717(99)00183-2},
  volume       = {32},
  year         = {2000},
}