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Heterogeneous electron transfer studies with ligninolytic redox enzymes and living bacteria. Applications in biosensors and biofuel cells

Coman, Vasile LU (2009)
Abstract
The catalytic properties and the inter-domain electron transfer of cellobiose dehydrogenase (CDH) from the ascomycete fungus Myriococcum thermophilum adsorbed on graphite and thiol (SAM) modified gold electrodes were investigated using cyclic voltammetry, flow injection

amperometry and UV-Vis spectroelectrochemistry. The fabrication and characterisation of a noncompartmentalised, mediator and cofactor free glucose–oxygen biofuel cell well-operating in glucose-containing buffers and human blood serum was performed. The biofuel cell was based on adsorbed enzymes exhibiting direct bioelectrocatalysis, viz. CDH from Dichomera saubinetii and Corynascus thermophilus used as anodic bioelements and laccase (Lc) from Trametes hirsuta and... (More)
The catalytic properties and the inter-domain electron transfer of cellobiose dehydrogenase (CDH) from the ascomycete fungus Myriococcum thermophilum adsorbed on graphite and thiol (SAM) modified gold electrodes were investigated using cyclic voltammetry, flow injection

amperometry and UV-Vis spectroelectrochemistry. The fabrication and characterisation of a noncompartmentalised, mediator and cofactor free glucose–oxygen biofuel cell well-operating in glucose-containing buffers and human blood serum was performed. The biofuel cell was based on adsorbed enzymes exhibiting direct bioelectrocatalysis, viz. CDH from Dichomera saubinetii and Corynascus thermophilus used as anodic bioelements and laccase (Lc) from Trametes hirsuta and bilirubin oxidase (BOD) from Myrothecium verrucaria used as cathodic bioelements, respectively.

Different bacterial strains of E. coli and B. subtilis were immobilised on the electrode surface and tested for electrical communication using soluble and polymeric mediators. In the case of E. coli, the introduction of cytochromes in the inner membrane facilitated the electrochemical communication when using artificial mediators, while in the case of B. subtilis, the results demonstrated that mediators did not have to pass the cytosolic membrane to bring about an efficient electronic communication between bacterial cells with a thick cell wall and electrodes. (Less)
Please use this url to cite or link to this publication:
author
supervisor
opponent
  • Professor Dr. Shin, Woonsup, Department of Chemistry and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul, Republic of Korea
organization
publishing date
type
Thesis
publication status
published
subject
keywords
cellobiose dehydrogenase, direct electron transfer, mediated electron transfer, self-assembled monolayer, flow injection analysis, amperometric biosensor, E. coli, succinate:quinone reductase, biofuel cell, B. subtilis, whole-cell biosensor
pages
184 pages
publisher
Lund University
defense location
Lecture Hall B, Centre for Chemistry and Chemical Enginering, Sölvegatan 39, Lund
defense date
2009-09-25 10:30
ISBN
978-91-7422-229-6
language
English
LU publication?
yes
id
b60056f0-99b1-4801-8e23-f97b68d02b67 (old id 1468854)
date added to LUP
2009-09-01 14:37:53
date last changed
2016-09-19 08:45:05
@phdthesis{b60056f0-99b1-4801-8e23-f97b68d02b67,
  abstract     = {The catalytic properties and the inter-domain electron transfer of cellobiose dehydrogenase (CDH) from the ascomycete fungus Myriococcum thermophilum adsorbed on graphite and thiol (SAM) modified gold electrodes were investigated using cyclic voltammetry, flow injection<br/><br>
amperometry and UV-Vis spectroelectrochemistry. The fabrication and characterisation of a noncompartmentalised, mediator and cofactor free glucose–oxygen biofuel cell well-operating in glucose-containing buffers and human blood serum was performed. The biofuel cell was based on adsorbed enzymes exhibiting direct bioelectrocatalysis, viz. CDH from Dichomera saubinetii and Corynascus thermophilus used as anodic bioelements and laccase (Lc) from Trametes hirsuta and bilirubin oxidase (BOD) from Myrothecium verrucaria used as cathodic bioelements, respectively. <br/><br>
Different bacterial strains of E. coli and B. subtilis were immobilised on the electrode surface and tested for electrical communication using soluble and polymeric mediators. In the case of E. coli, the introduction of cytochromes in the inner membrane facilitated the electrochemical communication when using artificial mediators, while in the case of B. subtilis, the results demonstrated that mediators did not have to pass the cytosolic membrane to bring about an efficient electronic communication between bacterial cells with a thick cell wall and electrodes.},
  author       = {Coman, Vasile},
  isbn         = {978-91-7422-229-6},
  keyword      = {cellobiose dehydrogenase,direct electron transfer,mediated electron transfer,self-assembled monolayer,flow injection analysis,amperometric biosensor,E. coli,succinate:quinone reductase,biofuel cell,B. subtilis,whole-cell biosensor},
  language     = {eng},
  pages        = {184},
  publisher    = {Lund University},
  school       = {Lund University},
  title        = {Heterogeneous electron transfer studies with ligninolytic redox enzymes and living bacteria. Applications in biosensors and biofuel cells},
  year         = {2009},
}