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Visualisation of ectomycorrhizal rhizomorph structure using laser scanning confocal microscopy

Schweiger, P F; Rouhier, Hervé LU and Söderström, Bengt LU (2002) In Mycological Research1989-01-01+01:002010-01-01+01:00 106(3). p.349-354
Abstract
A method for the observation of the three-dimensional structure of intact ectomycorrhizal rhizomorphs is described. The method is based on a combination of clearing the material with KOH followed by staining with congo red and subsequent imaging under a laser scanning confocal microscope (LSCM). The images obtained are of a much higher three-dimensional resolution than those obtained previously by use of conventional light microscopical techniques. The structure of highly differentiated and undifferentiated rhizomorphs is described. Applications of the method are briefly discussed.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Mycological Research1989-01-01+01:002010-01-01+01:00
volume
106
issue
3
pages
349 - 354
publisher
Elsevier
external identifiers
  • wos:000176325400013
  • scopus:0036276542
ISSN
1469-8102
DOI
10.1017/S0953756202005579
language
English
LU publication?
yes
id
1c1dc5d3-e578-4c26-8c94-ae1420466449 (old id 146907)
date added to LUP
2007-06-29 14:39:21
date last changed
2017-11-28 14:56:33
@article{1c1dc5d3-e578-4c26-8c94-ae1420466449,
  abstract     = {A method for the observation of the three-dimensional structure of intact ectomycorrhizal rhizomorphs is described. The method is based on a combination of clearing the material with KOH followed by staining with congo red and subsequent imaging under a laser scanning confocal microscope (LSCM). The images obtained are of a much higher three-dimensional resolution than those obtained previously by use of conventional light microscopical techniques. The structure of highly differentiated and undifferentiated rhizomorphs is described. Applications of the method are briefly discussed.},
  author       = {Schweiger, P F and Rouhier, Hervé and Söderström, Bengt},
  issn         = {1469-8102},
  language     = {eng},
  number       = {3},
  pages        = {349--354},
  publisher    = {Elsevier},
  series       = {Mycological Research1989-01-01+01:002010-01-01+01:00},
  title        = {Visualisation of ectomycorrhizal rhizomorph structure using laser scanning confocal microscopy},
  url          = {http://dx.doi.org/10.1017/S0953756202005579},
  volume       = {106},
  year         = {2002},
}