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Analysis of proteins in the extracellular matrix of the plant pathogenic fungus Bipolaris sorokiniana using 2-D gel electrophoresis and MS/MS

Apoga, D; Ek, B and Tunlid, Anders LU (2001) In FEMS Microbiology Letters 197(2). p.145-150
Abstract
A method was developed for isolating and sequencing proteins present in the extracellular matrix (ECM) of germlings and hyphae of filamentous fungi, Surface proteins of the cereal pathogen Bipolaris sorokiniana were labelled with a membrane impermeable biotinylating agent and extracted using a glycine-HCl buffer. Extracted proteins were purified by affinity binding to streptavidin-conjugated magnetic beads or by two-dimensional gel electrophoresis. Four of the biotinylated proteins from the ECM of B. sorokiniana were isolated, in gel digested with trypsin and partly sequenced by tandem mass spectrometry. No significant sequence similarities to proteins in databases were obtained. (C) 2001 Federation of European Microbiological Societies.... (More)
A method was developed for isolating and sequencing proteins present in the extracellular matrix (ECM) of germlings and hyphae of filamentous fungi, Surface proteins of the cereal pathogen Bipolaris sorokiniana were labelled with a membrane impermeable biotinylating agent and extracted using a glycine-HCl buffer. Extracted proteins were purified by affinity binding to streptavidin-conjugated magnetic beads or by two-dimensional gel electrophoresis. Four of the biotinylated proteins from the ECM of B. sorokiniana were isolated, in gel digested with trypsin and partly sequenced by tandem mass spectrometry. No significant sequence similarities to proteins in databases were obtained. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights, reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
FEMS Microbiology Letters
volume
197
issue
2
pages
145 - 150
publisher
Elsevier
external identifiers
  • scopus:0035853471
ISSN
1574-6968
DOI
10.1016/S0378-1097(01)00052-0
language
English
LU publication?
yes
id
ad86dd9e-2ec5-4513-9fb0-486256255db5 (old id 146963)
date added to LUP
2007-06-29 09:01:45
date last changed
2018-05-29 10:37:16
@article{ad86dd9e-2ec5-4513-9fb0-486256255db5,
  abstract     = {A method was developed for isolating and sequencing proteins present in the extracellular matrix (ECM) of germlings and hyphae of filamentous fungi, Surface proteins of the cereal pathogen Bipolaris sorokiniana were labelled with a membrane impermeable biotinylating agent and extracted using a glycine-HCl buffer. Extracted proteins were purified by affinity binding to streptavidin-conjugated magnetic beads or by two-dimensional gel electrophoresis. Four of the biotinylated proteins from the ECM of B. sorokiniana were isolated, in gel digested with trypsin and partly sequenced by tandem mass spectrometry. No significant sequence similarities to proteins in databases were obtained. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights, reserved.},
  author       = {Apoga, D and Ek, B and Tunlid, Anders},
  issn         = {1574-6968},
  language     = {eng},
  number       = {2},
  pages        = {145--150},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Letters},
  title        = {Analysis of proteins in the extracellular matrix of the plant pathogenic fungus Bipolaris sorokiniana using 2-D gel electrophoresis and MS/MS},
  url          = {http://dx.doi.org/10.1016/S0378-1097(01)00052-0},
  volume       = {197},
  year         = {2001},
}