Analysis of proteins in the extracellular matrix of the plant pathogenic fungus Bipolaris sorokiniana using 2-D gel electrophoresis and MS/MS
(2001) In FEMS Microbiology Letters 197(2). p.145-150- Abstract
- A method was developed for isolating and sequencing proteins present in the extracellular matrix (ECM) of germlings and hyphae of filamentous fungi, Surface proteins of the cereal pathogen Bipolaris sorokiniana were labelled with a membrane impermeable biotinylating agent and extracted using a glycine-HCl buffer. Extracted proteins were purified by affinity binding to streptavidin-conjugated magnetic beads or by two-dimensional gel electrophoresis. Four of the biotinylated proteins from the ECM of B. sorokiniana were isolated, in gel digested with trypsin and partly sequenced by tandem mass spectrometry. No significant sequence similarities to proteins in databases were obtained. (C) 2001 Federation of European Microbiological Societies.... (More)
- A method was developed for isolating and sequencing proteins present in the extracellular matrix (ECM) of germlings and hyphae of filamentous fungi, Surface proteins of the cereal pathogen Bipolaris sorokiniana were labelled with a membrane impermeable biotinylating agent and extracted using a glycine-HCl buffer. Extracted proteins were purified by affinity binding to streptavidin-conjugated magnetic beads or by two-dimensional gel electrophoresis. Four of the biotinylated proteins from the ECM of B. sorokiniana were isolated, in gel digested with trypsin and partly sequenced by tandem mass spectrometry. No significant sequence similarities to proteins in databases were obtained. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights, reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/146963
- author
- Apoga, D ; Ek, B and Tunlid, Anders LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- in
- FEMS Microbiology Letters
- volume
- 197
- issue
- 2
- pages
- 145 - 150
- publisher
- Oxford University Press
- external identifiers
-
- scopus:0035853471
- ISSN
- 1574-6968
- DOI
- 10.1016/S0378-1097(01)00052-0
- language
- English
- LU publication?
- yes
- id
- ad86dd9e-2ec5-4513-9fb0-486256255db5 (old id 146963)
- date added to LUP
- 2016-04-01 16:00:15
- date last changed
- 2024-01-10 23:45:41
@article{ad86dd9e-2ec5-4513-9fb0-486256255db5, abstract = {{A method was developed for isolating and sequencing proteins present in the extracellular matrix (ECM) of germlings and hyphae of filamentous fungi, Surface proteins of the cereal pathogen Bipolaris sorokiniana were labelled with a membrane impermeable biotinylating agent and extracted using a glycine-HCl buffer. Extracted proteins were purified by affinity binding to streptavidin-conjugated magnetic beads or by two-dimensional gel electrophoresis. Four of the biotinylated proteins from the ECM of B. sorokiniana were isolated, in gel digested with trypsin and partly sequenced by tandem mass spectrometry. No significant sequence similarities to proteins in databases were obtained. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights, reserved.}}, author = {{Apoga, D and Ek, B and Tunlid, Anders}}, issn = {{1574-6968}}, language = {{eng}}, number = {{2}}, pages = {{145--150}}, publisher = {{Oxford University Press}}, series = {{FEMS Microbiology Letters}}, title = {{Analysis of proteins in the extracellular matrix of the plant pathogenic fungus Bipolaris sorokiniana using 2-D gel electrophoresis and MS/MS}}, url = {{http://dx.doi.org/10.1016/S0378-1097(01)00052-0}}, doi = {{10.1016/S0378-1097(01)00052-0}}, volume = {{197}}, year = {{2001}}, }