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Laminin alpha1 domains LG4-5 are essential for the complete differentiation of visceral endoderm.

Åkerlund, Mikael LU ; Carmignac, Virginie LU ; Schéele, Susanne LU and Durbeej-Hjalt, Madeleine LU (2009) In Cell and Tissue Research1974-01-01+01:00 338(1). p.129-137
Abstract
The heterotrimeric basement membrane protein laminin-111 is essential for early mouse embryogenesis. Its beta1 and gamma1 chains are crucial for endoderm differentiation and for the formation of basement membranes, whereas alpha1 chain null mice only lack the extraembryonic Reichert's membrane. Nevertheless, mice deficient in the cell-binding alpha1 globular domains 4-5 (LG4-5) have a more severe phenotype than animals devoid of the whole alpha1 chain, as these domains are required for the formation of a polarized ectoderm. However, the influence of the alpha1LG4-5 domains on endoderm differentiation is unclear. We have used microarray analysis to compare the expression profiles of normal and alpha1LG4-5-deficient embryoid bodies and show... (More)
The heterotrimeric basement membrane protein laminin-111 is essential for early mouse embryogenesis. Its beta1 and gamma1 chains are crucial for endoderm differentiation and for the formation of basement membranes, whereas alpha1 chain null mice only lack the extraembryonic Reichert's membrane. Nevertheless, mice deficient in the cell-binding alpha1 globular domains 4-5 (LG4-5) have a more severe phenotype than animals devoid of the whole alpha1 chain, as these domains are required for the formation of a polarized ectoderm. However, the influence of the alpha1LG4-5 domains on endoderm differentiation is unclear. We have used microarray analysis to compare the expression profiles of normal and alpha1LG4-5-deficient embryoid bodies and show that genes encoding secreted plasma proteins and proteins involved in endocytosis are reduced in alpha1LG4-5-deficient embryoid bodies, indicating incomplete differentiation of the visceral endoderm. Moreover, mice lacking alpha1LG4-5 display endoderm disorganization and a defective expression of the endoderm marker Dab2. We hypothesize that alpha1LG4-5 domains provide an autocrine signal necessary for the complete differentiation of a functional visceral endoderm and vital signals for the polarization of the epiblast. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cell and Tissue Research1974-01-01+01:00
volume
338
issue
1
pages
129 - 137
publisher
Springer
external identifiers
  • wos:000270737500011
  • pmid:19657675
  • scopus:70350006669
ISSN
1432-0878
DOI
10.1007/s00441-009-0845-3
language
English
LU publication?
yes
id
1ed5b3d7-cc4e-4669-8813-196ecac99378 (old id 1469981)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19657675?dopt=Abstract
date added to LUP
2009-09-07 15:09:20
date last changed
2017-01-01 04:48:17
@article{1ed5b3d7-cc4e-4669-8813-196ecac99378,
  abstract     = {The heterotrimeric basement membrane protein laminin-111 is essential for early mouse embryogenesis. Its beta1 and gamma1 chains are crucial for endoderm differentiation and for the formation of basement membranes, whereas alpha1 chain null mice only lack the extraembryonic Reichert's membrane. Nevertheless, mice deficient in the cell-binding alpha1 globular domains 4-5 (LG4-5) have a more severe phenotype than animals devoid of the whole alpha1 chain, as these domains are required for the formation of a polarized ectoderm. However, the influence of the alpha1LG4-5 domains on endoderm differentiation is unclear. We have used microarray analysis to compare the expression profiles of normal and alpha1LG4-5-deficient embryoid bodies and show that genes encoding secreted plasma proteins and proteins involved in endocytosis are reduced in alpha1LG4-5-deficient embryoid bodies, indicating incomplete differentiation of the visceral endoderm. Moreover, mice lacking alpha1LG4-5 display endoderm disorganization and a defective expression of the endoderm marker Dab2. We hypothesize that alpha1LG4-5 domains provide an autocrine signal necessary for the complete differentiation of a functional visceral endoderm and vital signals for the polarization of the epiblast.},
  author       = {Åkerlund, Mikael and Carmignac, Virginie and Schéele, Susanne and Durbeej-Hjalt, Madeleine},
  issn         = {1432-0878},
  language     = {eng},
  number       = {1},
  pages        = {129--137},
  publisher    = {Springer},
  series       = {Cell and Tissue Research1974-01-01+01:00},
  title        = {Laminin alpha1 domains LG4-5 are essential for the complete differentiation of visceral endoderm.},
  url          = {http://dx.doi.org/10.1007/s00441-009-0845-3},
  volume       = {338},
  year         = {2009},
}