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Halomonas andesensis sp. nov., a moderate halophile isolated from the saline lake Laguna Colorada in Bolivia.

Guzmán, Daniel LU ; Quillaguaman, Jorge LU ; Munoz, Marlene LU and Hatti-Kaul, Rajni LU (2010) In International Journal of Systematic and Evolutionary Microbiology 60. p.749-753
Abstract
Strain LC6T was isolated from a water sample of the lake Laguna Colorada in the Bolivian Andes. The bacterium was characterized as moderately halophilic, motile, Gram-negative rod. The major fatty acids present in the cell were C18:1omega7c, C16:1omega7c iso 2-OH, C16:0 and C12:0 3-OH. The respiratory ubiquinones found in strain LC6T were Q-9 (97%) and Q-8 (3%). The organism was found to be an aerobic, heterotroph, able to utilize various carbohydrates and other substrates as carbon source. The G+C content (mol %) of the genomic DNA was 52.5. The organism was able to grow in the pH range of 6.0-11.0 (optimum between 7.0-8.0), temperature of 4-45 degrees C (optimum at 30-35 degrees C) and sodium chloride concentration of 0.5-20 % (w/v)... (More)
Strain LC6T was isolated from a water sample of the lake Laguna Colorada in the Bolivian Andes. The bacterium was characterized as moderately halophilic, motile, Gram-negative rod. The major fatty acids present in the cell were C18:1omega7c, C16:1omega7c iso 2-OH, C16:0 and C12:0 3-OH. The respiratory ubiquinones found in strain LC6T were Q-9 (97%) and Q-8 (3%). The organism was found to be an aerobic, heterotroph, able to utilize various carbohydrates and other substrates as carbon source. The G+C content (mol %) of the genomic DNA was 52.5. The organism was able to grow in the pH range of 6.0-11.0 (optimum between 7.0-8.0), temperature of 4-45 degrees C (optimum at 30-35 degrees C) and sodium chloride concentration of 0.5-20 % (w/v) (optimum between 1-3% w/v). Based on 16S rRNA gene sequence analysis, LC6T was 98.8 % similar to Halomonas hydrothermalis DSM 15725T and Halomonas venusta DSM 4743T, and 98.4 % similar to Halomonas aquamarina DSM 30161T, Halomonas axialensis DSM 15723T and Halomonas meridiana DSM 5425T. However, its DNA-DNA relatedness with these type strains was considerably low. LC6T resembled other Halomonas species with respect to various physiological, biochemical, and nutritional characteristics. Combined phenotypic data and DNA-DNA hybridization data supported the conclusion that LC6T is a novel species of the genus Halomonas, for which the name Halomonas andesensis (LC6T=CCUG 54844T=LMG 24243T = DSM 19434T) is proposed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Systematic and Evolutionary Microbiology
volume
60
pages
749 - 753
publisher
Society for General Microbiology
external identifiers
  • wos:000277733300005
  • pmid:19656933
  • scopus:77950608743
ISSN
1466-5026
DOI
10.1099/ijs.0.014522-0
language
English
LU publication?
yes
id
4acadabc-300d-46b2-b22e-4fdd34fd2b5d (old id 1469988)
date added to LUP
2009-09-08 13:55:03
date last changed
2018-07-08 03:18:41
@article{4acadabc-300d-46b2-b22e-4fdd34fd2b5d,
  abstract     = {Strain LC6T was isolated from a water sample of the lake Laguna Colorada in the Bolivian Andes. The bacterium was characterized as moderately halophilic, motile, Gram-negative rod. The major fatty acids present in the cell were C18:1omega7c, C16:1omega7c iso 2-OH, C16:0 and C12:0 3-OH. The respiratory ubiquinones found in strain LC6T were Q-9 (97%) and Q-8 (3%). The organism was found to be an aerobic, heterotroph, able to utilize various carbohydrates and other substrates as carbon source. The G+C content (mol %) of the genomic DNA was 52.5. The organism was able to grow in the pH range of 6.0-11.0 (optimum between 7.0-8.0), temperature of 4-45 degrees C (optimum at 30-35 degrees C) and sodium chloride concentration of 0.5-20 % (w/v) (optimum between 1-3% w/v). Based on 16S rRNA gene sequence analysis, LC6T was 98.8 % similar to Halomonas hydrothermalis DSM 15725T and Halomonas venusta DSM 4743T, and 98.4 % similar to Halomonas aquamarina DSM 30161T, Halomonas axialensis DSM 15723T and Halomonas meridiana DSM 5425T. However, its DNA-DNA relatedness with these type strains was considerably low. LC6T resembled other Halomonas species with respect to various physiological, biochemical, and nutritional characteristics. Combined phenotypic data and DNA-DNA hybridization data supported the conclusion that LC6T is a novel species of the genus Halomonas, for which the name Halomonas andesensis (LC6T=CCUG 54844T=LMG 24243T = DSM 19434T) is proposed.},
  author       = {Guzmán, Daniel and Quillaguaman, Jorge and Munoz, Marlene and Hatti-Kaul, Rajni},
  issn         = {1466-5026},
  language     = {eng},
  pages        = {749--753},
  publisher    = {Society for General Microbiology},
  series       = {International Journal of Systematic and Evolutionary Microbiology},
  title        = {Halomonas andesensis sp. nov., a moderate halophile isolated from the saline lake Laguna Colorada in Bolivia.},
  url          = {http://dx.doi.org/10.1099/ijs.0.014522-0},
  volume       = {60},
  year         = {2010},
}