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Lipid and fatty acid composition of hyphae and spores of arbuscular mycorrhizal fungi at different growth stages

Olsson, Pål Axel LU and Johansen, A (2000) In Mycological Research 104(4). p.429-434
Abstract
The lipid and fatty acid compositions of Glomus intraradices and G. claroideum mycelia, extracted from quartz sand in a compartmentalized growth system, were analysed. The fungi were grown in association with Cucumis sativus and Trifolium subterraneum, respectively. For both fungi, the fatty acids 16:1 omega 5 and 16:0 dominated in the neutral lipid fraction, and 18:1 omega 7 made up a significant part of the phospholipids. The fatty acids were used as estimators of the amount of neutral lipids and phospholipids of AM fungi as well as to calculate the biomass of different parts of their mycelium. The phospholipid content was higher in hyphae than in spores, whereas the opposite was observed for neutral lipids. In 3-mo-old G. intraradices... (More)
The lipid and fatty acid compositions of Glomus intraradices and G. claroideum mycelia, extracted from quartz sand in a compartmentalized growth system, were analysed. The fungi were grown in association with Cucumis sativus and Trifolium subterraneum, respectively. For both fungi, the fatty acids 16:1 omega 5 and 16:0 dominated in the neutral lipid fraction, and 18:1 omega 7 made up a significant part of the phospholipids. The fatty acids were used as estimators of the amount of neutral lipids and phospholipids of AM fungi as well as to calculate the biomass of different parts of their mycelium. The phospholipid content was higher in hyphae than in spores, whereas the opposite was observed for neutral lipids. In 3-mo-old G. intraradices mycelium, spores accounted for 90% of the external biomass, and calculations indicated that about 20% of the spore biomass consisted of neutral lipids. In both fungi the fatty acid compositions of hyphae and spores were similar regardless of the age of the mycelium. Using the signature fatty acid 16:1 omega 5 to calculate the distribution of AM biomass for a 2-mo-old mycelium of G. claroideum, we found that the fungal biomass was equally distributed between the external mycelium and the internal mycelium in the host root. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Mycological Research
volume
104
issue
4
pages
429 - 434
publisher
Cambridge University Press
external identifiers
  • scopus:0034060379
ISSN
1469-8102
DOI
10.1017/S0953756299001410
language
English
LU publication?
yes
id
bd220f9d-1fac-41ff-9fb7-e806dcb47c81 (old id 147027)
date added to LUP
2007-06-29 14:12:18
date last changed
2017-08-06 03:31:22
@article{bd220f9d-1fac-41ff-9fb7-e806dcb47c81,
  abstract     = {The lipid and fatty acid compositions of Glomus intraradices and G. claroideum mycelia, extracted from quartz sand in a compartmentalized growth system, were analysed. The fungi were grown in association with Cucumis sativus and Trifolium subterraneum, respectively. For both fungi, the fatty acids 16:1 omega 5 and 16:0 dominated in the neutral lipid fraction, and 18:1 omega 7 made up a significant part of the phospholipids. The fatty acids were used as estimators of the amount of neutral lipids and phospholipids of AM fungi as well as to calculate the biomass of different parts of their mycelium. The phospholipid content was higher in hyphae than in spores, whereas the opposite was observed for neutral lipids. In 3-mo-old G. intraradices mycelium, spores accounted for 90% of the external biomass, and calculations indicated that about 20% of the spore biomass consisted of neutral lipids. In both fungi the fatty acid compositions of hyphae and spores were similar regardless of the age of the mycelium. Using the signature fatty acid 16:1 omega 5 to calculate the distribution of AM biomass for a 2-mo-old mycelium of G. claroideum, we found that the fungal biomass was equally distributed between the external mycelium and the internal mycelium in the host root.},
  author       = {Olsson, Pål Axel and Johansen, A},
  issn         = {1469-8102},
  language     = {eng},
  number       = {4},
  pages        = {429--434},
  publisher    = {Cambridge University Press},
  series       = {Mycological Research},
  title        = {Lipid and fatty acid composition of hyphae and spores of arbuscular mycorrhizal fungi at different growth stages},
  url          = {http://dx.doi.org/10.1017/S0953756299001410},
  volume       = {104},
  year         = {2000},
}