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An oscillating manganese electron paramagnetic resonance signal from the S0 state of the oxygen evolving complex in Photosystem II

Åhrling, Karin A.; Peterson Årsköld, Sindra LU and Styring, Stenbjörn LU (1997) In Biochemistry 36(43). p.13148-13152
Abstract
Photosynthesis produces the oxygen necessary for all aerobic life. During this process, the manganese-containing oxygen evolving complex (OEC) in photosystem II (PSII), cycles through five oxidation states, S0-S4. One of these, S2, is known to be paramagnetic and gives rise to electron paramagnetic resonance (EPR) signals used to probe the catalytic structure and function of the OEC. The S0 state has long been thought to be paramagnetic. We report here a Mn EPR signal from the previously EPR invisible S0 state. The new signal oscillates with a period of four, indicating that it originates from fully active PSII centers. Although similar to the S2 state multiline signal, the new signal is wider (2200

gauss compared with 1850 gauss... (More)
Photosynthesis produces the oxygen necessary for all aerobic life. During this process, the manganese-containing oxygen evolving complex (OEC) in photosystem II (PSII), cycles through five oxidation states, S0-S4. One of these, S2, is known to be paramagnetic and gives rise to electron paramagnetic resonance (EPR) signals used to probe the catalytic structure and function of the OEC. The S0 state has long been thought to be paramagnetic. We report here a Mn EPR signal from the previously EPR invisible S0 state. The new signal oscillates with a period of four, indicating that it originates from fully active PSII centers. Although similar to the S2 state multiline signal, the new signal is wider (2200

gauss compared with 1850 gauss in samples produced by flashing), with different peak intensity and separation (82 gauss compared with 89 gauss). These characteristics are consistent with the S0 state EPR signal arising from a coupled MnII-MnIII intermediate. The new signal is more stable than the S2 state signal and its decay in tens of minutes is indicative of it originating from the S0 state. The S0 state signal will provide invaluable information toward the understanding of oxygen evolution in plants. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemistry
volume
36
issue
43
pages
13148 - 13152
publisher
The American Chemical Society
external identifiers
  • scopus:0030736075
ISSN
0006-2960
DOI
10.1021/bi971815w
language
English
LU publication?
yes
id
5661e611-f616-48b3-851f-2f11c061f95a (old id 1471452)
date added to LUP
2009-09-03 12:22:36
date last changed
2017-05-21 03:40:37
@article{5661e611-f616-48b3-851f-2f11c061f95a,
  abstract     = {Photosynthesis produces the oxygen necessary for all aerobic life. During this process, the manganese-containing oxygen evolving complex (OEC) in photosystem II (PSII), cycles through five oxidation states, S0-S4. One of these, S2, is known to be paramagnetic and gives rise to electron paramagnetic resonance (EPR) signals used to probe the catalytic structure and function of the OEC. The S0 state has long been thought to be paramagnetic. We report here a Mn EPR signal from the previously EPR invisible S0 state. The new signal oscillates with a period of four, indicating that it originates from fully active PSII centers. Although similar to the S2 state multiline signal, the new signal is wider (2200<br/><br>
gauss compared with 1850 gauss in samples produced by flashing), with different peak intensity and separation (82 gauss compared with 89 gauss). These characteristics are consistent with the S0 state EPR signal arising from a coupled MnII-MnIII intermediate. The new signal is more stable than the S2 state signal and its decay in tens of minutes is indicative of it originating from the S0 state. The S0 state signal will provide invaluable information toward the understanding of oxygen evolution in plants.},
  author       = {Åhrling, Karin A. and Peterson Årsköld, Sindra and Styring, Stenbjörn},
  issn         = {0006-2960},
  language     = {eng},
  number       = {43},
  pages        = {13148--13152},
  publisher    = {The American Chemical Society},
  series       = {Biochemistry},
  title        = {An oscillating manganese electron paramagnetic resonance signal from the S0 state of the oxygen evolving complex in Photosystem II},
  url          = {http://dx.doi.org/10.1021/bi971815w},
  volume       = {36},
  year         = {1997},
}