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Partition by countercurrent distribution (CCD)

Åkerlund, Hans-Erik LU (2000) In Aqueous two-phase systems : mehods and protocols p.55-64
Abstract
A crucial step in many biochemical studies is the separation and purification of the individual structures of interest. For cells, cell organelles, and membrane fragments, the use of aqueous two-phase partition has become an important tool. The basis for separation in these phase systems is the surface properties of the material, and the method, therefore, nicely complements the more traditional centrifugation techniques. Excellent separations have sometimes been obtained in just one or a few partition steps. However, in most instances, a single partition step only gives a partial or incomplete separation of components. By a multiple-extraction procedure, the resolving power can be drastically increased and components with only small... (More)
A crucial step in many biochemical studies is the separation and purification of the individual structures of interest. For cells, cell organelles, and membrane fragments, the use of aqueous two-phase partition has become an important tool. The basis for separation in these phase systems is the surface properties of the material, and the method, therefore, nicely complements the more traditional centrifugation techniques. Excellent separations have sometimes been obtained in just one or a few partition steps. However, in most instances, a single partition step only gives a partial or incomplete separation of components. By a multiple-extraction procedure, the resolving power can be drastically increased and components with only small differences in surface properties can be separated. (Less)
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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
in
Aqueous two-phase systems : mehods and protocols
editor
Hatti-Kaul, Rajni and
pages
55 - 64
publisher
Human Press Inc, Totowa, NJ
ISBN
0-89603-541-7
DOI
10.1385/1-59259-028-4:55
language
English
LU publication?
yes
id
91aa59c6-6500-4483-a799-bd7d1955a87e (old id 1474807)
date added to LUP
2009-09-29 18:07:25
date last changed
2016-04-16 07:18:55
@inbook{91aa59c6-6500-4483-a799-bd7d1955a87e,
  abstract     = {A crucial step in many biochemical studies is the separation and purification of the individual structures of interest. For cells, cell organelles, and membrane fragments, the use of aqueous two-phase partition has become an important tool. The basis for separation in these phase systems is the surface properties of the material, and the method, therefore, nicely complements the more traditional centrifugation techniques. Excellent separations have sometimes been obtained in just one or a few partition steps. However, in most instances, a single partition step only gives a partial or incomplete separation of components. By a multiple-extraction procedure, the resolving power can be drastically increased and components with only small differences in surface properties can be separated.},
  author       = {Åkerlund, Hans-Erik},
  editor       = {Hatti-Kaul, Rajni},
  isbn         = {0-89603-541-7},
  language     = {eng},
  pages        = {55--64},
  publisher    = {Human Press Inc, Totowa, NJ},
  series       = {Aqueous two-phase systems : mehods and protocols},
  title        = {Partition by countercurrent distribution (CCD)},
  url          = {http://dx.doi.org/10.1385/1-59259-028-4:55},
  year         = {2000},
}