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Dried-Reswollen Immobilized Biocatalysts for Detoxification of Organophosphorous Compounds in the Flow Systems

Efremenko, Elena N.; Lyagin, Ilya V.; Plieva, Fatima LU ; Galaev, Igor LU and Mattiasson, Bo LU (2009) In Applied Biochemistry and Biotechnology 159(1). p.251-260
Abstract
New immobilized biocatalysts based on polypeptides containing N- or C-terminal polyhistidine sequences and possessing organophosphorus hydrolase activity were investigated for detoxification of organophosphorous neurotoxic compounds in the flow systems. The biocatalysts were revealed to have a high catalytic activity within wide pH and temperature ranges 7.5-12.5 A degrees C and 15-65 A degrees C, respectively. The immobilized biocatalysts can be dried and reswollen before use with 92-93% catalytic activity remaining after drying and rehydration procedures. The half-lives of the biocatalysts under wet and dry storage conditions were 420 and 540 days, respectively.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
immobilized biocatalyst, Dried/reswollen, Organophosphorus hydrolase, Polyhistidine sequence, Organophosphorous compound
in
Applied Biochemistry and Biotechnology
volume
159
issue
1
pages
251 - 260
publisher
Humana Press
external identifiers
  • wos:000269515600023
  • scopus:70350568768
ISSN
1559-0291
DOI
10.1007/s12010-009-8564-5
language
English
LU publication?
yes
id
db8593cf-3a0b-49e3-830e-55ec1b621787 (old id 1476816)
date added to LUP
2009-09-24 11:13:04
date last changed
2017-01-01 04:49:15
@article{db8593cf-3a0b-49e3-830e-55ec1b621787,
  abstract     = {New immobilized biocatalysts based on polypeptides containing N- or C-terminal polyhistidine sequences and possessing organophosphorus hydrolase activity were investigated for detoxification of organophosphorous neurotoxic compounds in the flow systems. The biocatalysts were revealed to have a high catalytic activity within wide pH and temperature ranges 7.5-12.5 A degrees C and 15-65 A degrees C, respectively. The immobilized biocatalysts can be dried and reswollen before use with 92-93% catalytic activity remaining after drying and rehydration procedures. The half-lives of the biocatalysts under wet and dry storage conditions were 420 and 540 days, respectively.},
  author       = {Efremenko, Elena N. and Lyagin, Ilya V. and Plieva, Fatima and Galaev, Igor and Mattiasson, Bo},
  issn         = {1559-0291},
  keyword      = {immobilized biocatalyst,Dried/reswollen,Organophosphorus hydrolase,Polyhistidine sequence,Organophosphorous compound},
  language     = {eng},
  number       = {1},
  pages        = {251--260},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology},
  title        = {Dried-Reswollen Immobilized Biocatalysts for Detoxification of Organophosphorous Compounds in the Flow Systems},
  url          = {http://dx.doi.org/10.1007/s12010-009-8564-5},
  volume       = {159},
  year         = {2009},
}