Dried-Reswollen Immobilized Biocatalysts for Detoxification of Organophosphorous Compounds in the Flow Systems
(2009) In Applied Biochemistry and Biotechnology 159(1). p.251-260- Abstract
- New immobilized biocatalysts based on polypeptides containing N- or C-terminal polyhistidine sequences and possessing organophosphorus hydrolase activity were investigated for detoxification of organophosphorous neurotoxic compounds in the flow systems. The biocatalysts were revealed to have a high catalytic activity within wide pH and temperature ranges 7.5-12.5 A degrees C and 15-65 A degrees C, respectively. The immobilized biocatalysts can be dried and reswollen before use with 92-93% catalytic activity remaining after drying and rehydration procedures. The half-lives of the biocatalysts under wet and dry storage conditions were 420 and 540 days, respectively.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1476816
- author
- Efremenko, Elena N. ; Lyagin, Ilya V. ; Plieva, Fatima LU ; Galaev, Igor LU and Mattiasson, Bo LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- immobilized biocatalyst, Dried/reswollen, Organophosphorus hydrolase, Polyhistidine sequence, Organophosphorous compound
- in
- Applied Biochemistry and Biotechnology
- volume
- 159
- issue
- 1
- pages
- 251 - 260
- publisher
- Humana Press
- external identifiers
-
- wos:000269515600023
- scopus:70350568768
- pmid:19551352
- ISSN
- 1559-0291
- DOI
- 10.1007/s12010-009-8564-5
- language
- English
- LU publication?
- yes
- id
- db8593cf-3a0b-49e3-830e-55ec1b621787 (old id 1476816)
- date added to LUP
- 2016-04-01 12:05:02
- date last changed
- 2022-02-18 17:38:48
@article{db8593cf-3a0b-49e3-830e-55ec1b621787, abstract = {{New immobilized biocatalysts based on polypeptides containing N- or C-terminal polyhistidine sequences and possessing organophosphorus hydrolase activity were investigated for detoxification of organophosphorous neurotoxic compounds in the flow systems. The biocatalysts were revealed to have a high catalytic activity within wide pH and temperature ranges 7.5-12.5 A degrees C and 15-65 A degrees C, respectively. The immobilized biocatalysts can be dried and reswollen before use with 92-93% catalytic activity remaining after drying and rehydration procedures. The half-lives of the biocatalysts under wet and dry storage conditions were 420 and 540 days, respectively.}}, author = {{Efremenko, Elena N. and Lyagin, Ilya V. and Plieva, Fatima and Galaev, Igor and Mattiasson, Bo}}, issn = {{1559-0291}}, keywords = {{immobilized biocatalyst; Dried/reswollen; Organophosphorus hydrolase; Polyhistidine sequence; Organophosphorous compound}}, language = {{eng}}, number = {{1}}, pages = {{251--260}}, publisher = {{Humana Press}}, series = {{Applied Biochemistry and Biotechnology}}, title = {{Dried-Reswollen Immobilized Biocatalysts for Detoxification of Organophosphorous Compounds in the Flow Systems}}, url = {{http://dx.doi.org/10.1007/s12010-009-8564-5}}, doi = {{10.1007/s12010-009-8564-5}}, volume = {{159}}, year = {{2009}}, }