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Mixed Protein Carriers for Modulating DNA Release

Moran, M. Carmen; Pais, Alberto A. C. C.; Ramalho, Amilcar; Miguel, M. Graca and Lindman, Björn LU (2009) In Langmuir 25(17). p.10263-10270
Abstract
Aqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited in forming DNA gel particles by interfacial diffusion. We report here the formation of DNA gel particles by mixing solutions of double-stranded DNA with aqueous solutions containing two cationic proteins, lysozyme and protamine sulfate. The effect of the lysozyme/protamine ratio on the degree of DNA entrapment, surface morphology, swelling-deswelling behavior, and kinetics of DNA release has been investigated. By mixing the two proteins, we obtain particles that display higher loading efficiency and loading capacity values, in comparison to those obtained in... (More)
Aqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited in forming DNA gel particles by interfacial diffusion. We report here the formation of DNA gel particles by mixing solutions of double-stranded DNA with aqueous solutions containing two cationic proteins, lysozyme and protamine sulfate. The effect of the lysozyme/protamine ratio on the degree of DNA entrapment, surface morphology, swelling-deswelling behavior, and kinetics of DNA release has been investigated. By mixing the two proteins, we obtain particles that display higher loading efficiency and loading capacity values, in comparison to those obtained in single-protein systems. Examination of the release profiles has shown that in mixed protein particles, complex, dual-stage release kinetics is obtained. The overall release profile is dependent on the lysozyme/protamine ratio. The obtained profiles, or segments of them, are accuratelly fitted using the zero-order and first-order models, and the Weibull function. Fluorescence microscopy studies have suggested that the formation of these particles is associated with the conservation of the secondary structure of DNA. This study presents a new platform for controlled release of DNA from DNA gel particles Conned by interfacial diffusion. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
25
issue
17
pages
10263 - 10270
publisher
The American Chemical Society
external identifiers
  • wos:000269197500089
  • scopus:69949114006
ISSN
0743-7463
DOI
10.1021/la901071v
language
English
LU publication?
yes
id
d1133e07-da5a-4c21-b2f4-04331295575d (old id 1476936)
date added to LUP
2009-09-24 10:24:46
date last changed
2017-01-01 04:23:40
@article{d1133e07-da5a-4c21-b2f4-04331295575d,
  abstract     = {Aqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited in forming DNA gel particles by interfacial diffusion. We report here the formation of DNA gel particles by mixing solutions of double-stranded DNA with aqueous solutions containing two cationic proteins, lysozyme and protamine sulfate. The effect of the lysozyme/protamine ratio on the degree of DNA entrapment, surface morphology, swelling-deswelling behavior, and kinetics of DNA release has been investigated. By mixing the two proteins, we obtain particles that display higher loading efficiency and loading capacity values, in comparison to those obtained in single-protein systems. Examination of the release profiles has shown that in mixed protein particles, complex, dual-stage release kinetics is obtained. The overall release profile is dependent on the lysozyme/protamine ratio. The obtained profiles, or segments of them, are accuratelly fitted using the zero-order and first-order models, and the Weibull function. Fluorescence microscopy studies have suggested that the formation of these particles is associated with the conservation of the secondary structure of DNA. This study presents a new platform for controlled release of DNA from DNA gel particles Conned by interfacial diffusion.},
  author       = {Moran, M. Carmen and Pais, Alberto A. C. C. and Ramalho, Amilcar and Miguel, M. Graca and Lindman, Björn},
  issn         = {0743-7463},
  language     = {eng},
  number       = {17},
  pages        = {10263--10270},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {Mixed Protein Carriers for Modulating DNA Release},
  url          = {http://dx.doi.org/10.1021/la901071v},
  volume       = {25},
  year         = {2009},
}