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The observation of nano-crystalline calcium phosphate precipitate in a simple supersaturated inorganic blood serum model - composition and morphology

Sandin, Karin ; Kloo, Lars ; Odselius, Rolf LU and Olsson, Lars-Fride (2009) In Journal of Applied Biomaterials and Biomechanics 7(1). p.13-22
Abstract
Aim. Calcium phosphate deposition in blood vessels is correlated to increased mortality risk. In this study, the formation of solid calcium phosphate in an in vitro solution mimicking the inorganic part of blood serum was studied. Methods. The precipitates formed were analyzed using several experimental techniques, including infrared spectroscopy, Raman spectroscopy, X-ray energy dispersive spectroscopy, chemical analysis of combustion gases, thermogravimetric analysis, as well as transmission electron microscopy and scanning electron microscopy. Results. The results indicate a microscopically amorphous but nano-crystalline material with an overall apatite structure. A plausible stoichiometry was determined to Ca-5(PO4)(3)(HCO3)center dot... (More)
Aim. Calcium phosphate deposition in blood vessels is correlated to increased mortality risk. In this study, the formation of solid calcium phosphate in an in vitro solution mimicking the inorganic part of blood serum was studied. Methods. The precipitates formed were analyzed using several experimental techniques, including infrared spectroscopy, Raman spectroscopy, X-ray energy dispersive spectroscopy, chemical analysis of combustion gases, thermogravimetric analysis, as well as transmission electron microscopy and scanning electron microscopy. Results. The results indicate a microscopically amorphous but nano-crystalline material with an overall apatite structure. A plausible stoichiometry was determined to Ca-5(PO4)(3)(HCO3)center dot 4H(2)O with an estimated solubility constant of 6.10(-39) (mol/L)(9). Bicarbonate in the water solution was shown to be essential for the precipitation, giving implications for in vitro studies. Conclusions. The calcium phosphate formed in this study shows many similarities to pathological calcium phosphates regarding composition, morphology and crystallinity. (Journal of Applied Biomaterials & Biomechanics 2009; 7: 13-22) (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Nano-crystalline, Mineralization, Apatite, Carbonated apatite
in
Journal of Applied Biomaterials and Biomechanics
volume
7
issue
1
pages
13 - 22
publisher
Wichtig Editore
external identifiers
  • wos:000269103400002
  • scopus:68149130919
ISSN
1724-6024
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Electron Microscopy Unit (013100002)
id
ddae3f90-788f-40a6-87a0-869504588b2c (old id 1477033)
date added to LUP
2016-04-01 11:59:41
date last changed
2021-06-08 01:47:16
@article{ddae3f90-788f-40a6-87a0-869504588b2c,
  abstract     = {Aim. Calcium phosphate deposition in blood vessels is correlated to increased mortality risk. In this study, the formation of solid calcium phosphate in an in vitro solution mimicking the inorganic part of blood serum was studied. Methods. The precipitates formed were analyzed using several experimental techniques, including infrared spectroscopy, Raman spectroscopy, X-ray energy dispersive spectroscopy, chemical analysis of combustion gases, thermogravimetric analysis, as well as transmission electron microscopy and scanning electron microscopy. Results. The results indicate a microscopically amorphous but nano-crystalline material with an overall apatite structure. A plausible stoichiometry was determined to Ca-5(PO4)(3)(HCO3)center dot 4H(2)O with an estimated solubility constant of 6.10(-39) (mol/L)(9). Bicarbonate in the water solution was shown to be essential for the precipitation, giving implications for in vitro studies. Conclusions. The calcium phosphate formed in this study shows many similarities to pathological calcium phosphates regarding composition, morphology and crystallinity. (Journal of Applied Biomaterials & Biomechanics 2009; 7: 13-22)},
  author       = {Sandin, Karin and Kloo, Lars and Odselius, Rolf and Olsson, Lars-Fride},
  issn         = {1724-6024},
  language     = {eng},
  number       = {1},
  pages        = {13--22},
  publisher    = {Wichtig Editore},
  series       = {Journal of Applied Biomaterials and Biomechanics},
  title        = {The observation of nano-crystalline calcium phosphate precipitate in a simple supersaturated inorganic blood serum model - composition and morphology},
  volume       = {7},
  year         = {2009},
}