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The observation of nano-crystalline calcium phosphate precipitate in a simple supersaturated inorganic blood serum model - composition and morphology

Sandin, Karin; Kloo, Lars; Odselius, Rolf LU and Olsson, Lars-Fride (2009) In Journal of Applied Biomaterials and Biomechanics 7(1). p.13-22
Abstract
Aim. Calcium phosphate deposition in blood vessels is correlated to increased mortality risk. In this study, the formation of solid calcium phosphate in an in vitro solution mimicking the inorganic part of blood serum was studied. Methods. The precipitates formed were analyzed using several experimental techniques, including infrared spectroscopy, Raman spectroscopy, X-ray energy dispersive spectroscopy, chemical analysis of combustion gases, thermogravimetric analysis, as well as transmission electron microscopy and scanning electron microscopy. Results. The results indicate a microscopically amorphous but nano-crystalline material with an overall apatite structure. A plausible stoichiometry was determined to Ca-5(PO4)(3)(HCO3)center dot... (More)
Aim. Calcium phosphate deposition in blood vessels is correlated to increased mortality risk. In this study, the formation of solid calcium phosphate in an in vitro solution mimicking the inorganic part of blood serum was studied. Methods. The precipitates formed were analyzed using several experimental techniques, including infrared spectroscopy, Raman spectroscopy, X-ray energy dispersive spectroscopy, chemical analysis of combustion gases, thermogravimetric analysis, as well as transmission electron microscopy and scanning electron microscopy. Results. The results indicate a microscopically amorphous but nano-crystalline material with an overall apatite structure. A plausible stoichiometry was determined to Ca-5(PO4)(3)(HCO3)center dot 4H(2)O with an estimated solubility constant of 6.10(-39) (mol/L)(9). Bicarbonate in the water solution was shown to be essential for the precipitation, giving implications for in vitro studies. Conclusions. The calcium phosphate formed in this study shows many similarities to pathological calcium phosphates regarding composition, morphology and crystallinity. (Journal of Applied Biomaterials & Biomechanics 2009; 7: 13-22) (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Nano-crystalline, Mineralization, Apatite, Carbonated apatite
in
Journal of Applied Biomaterials and Biomechanics
volume
7
issue
1
pages
13 - 22
publisher
Wichtig Editore
external identifiers
  • wos:000269103400002
  • scopus:68149130919
ISSN
1724-6024
language
English
LU publication?
yes
id
ddae3f90-788f-40a6-87a0-869504588b2c (old id 1477033)
date added to LUP
2009-09-24 11:04:29
date last changed
2017-01-01 04:44:15
@article{ddae3f90-788f-40a6-87a0-869504588b2c,
  abstract     = {Aim. Calcium phosphate deposition in blood vessels is correlated to increased mortality risk. In this study, the formation of solid calcium phosphate in an in vitro solution mimicking the inorganic part of blood serum was studied. Methods. The precipitates formed were analyzed using several experimental techniques, including infrared spectroscopy, Raman spectroscopy, X-ray energy dispersive spectroscopy, chemical analysis of combustion gases, thermogravimetric analysis, as well as transmission electron microscopy and scanning electron microscopy. Results. The results indicate a microscopically amorphous but nano-crystalline material with an overall apatite structure. A plausible stoichiometry was determined to Ca-5(PO4)(3)(HCO3)center dot 4H(2)O with an estimated solubility constant of 6.10(-39) (mol/L)(9). Bicarbonate in the water solution was shown to be essential for the precipitation, giving implications for in vitro studies. Conclusions. The calcium phosphate formed in this study shows many similarities to pathological calcium phosphates regarding composition, morphology and crystallinity. (Journal of Applied Biomaterials & Biomechanics 2009; 7: 13-22)},
  author       = {Sandin, Karin and Kloo, Lars and Odselius, Rolf and Olsson, Lars-Fride},
  issn         = {1724-6024},
  keyword      = {Nano-crystalline,Mineralization,Apatite,Carbonated apatite},
  language     = {eng},
  number       = {1},
  pages        = {13--22},
  publisher    = {Wichtig Editore},
  series       = {Journal of Applied Biomaterials and Biomechanics},
  title        = {The observation of nano-crystalline calcium phosphate precipitate in a simple supersaturated inorganic blood serum model - composition and morphology},
  volume       = {7},
  year         = {2009},
}