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Kinetics and metabolic versatility of highly tolerant phenol degrading Alcaligenes strain TW1.

Essam, Tamer LU ; Amin, Magdy Aly; Tayeb, Ossama El; Mattiasson, Bo LU and Guieysse, Benoit LU (2010) In Journal of Hazardous Materials 173. p.783-788
Abstract
A bacterium that could completely metabolize phenol in batch culture supplied with up to 1200mg phenoll(-1) at room temperature (25 degrees C) was isolated from the activated sludge of the industrial wastewater treatment plant of a Coke company (Cairo, Egypt). Morphological and physiological characterization showed strain TW1 was a motile, strictly aerobic, gram negative and short-rod occurring singly or in clusters. Partial 16S rRNA gene sequence analysis revealed strain TW1 belonged to the beta group of Proteobacteria, showing 100% identity to Alcaligenes SCTI. Strain TW1 aerobically grew on a number of monocyclic aromatic compounds (hydroquinone, catechol and o-cresol) as well as polycyclic aromatic compounds (pyrene, phenanthrene and... (More)
A bacterium that could completely metabolize phenol in batch culture supplied with up to 1200mg phenoll(-1) at room temperature (25 degrees C) was isolated from the activated sludge of the industrial wastewater treatment plant of a Coke company (Cairo, Egypt). Morphological and physiological characterization showed strain TW1 was a motile, strictly aerobic, gram negative and short-rod occurring singly or in clusters. Partial 16S rRNA gene sequence analysis revealed strain TW1 belonged to the beta group of Proteobacteria, showing 100% identity to Alcaligenes SCTI. Strain TW1 aerobically grew on a number of monocyclic aromatic compounds (hydroquinone, catechol and o-cresol) as well as polycyclic aromatic compounds (pyrene, phenanthrene and naphthalene). The growth of Alcaligenes TW1 on phenol as sole carbon and energy source (25 degrees C) was well described by the Haldane kinetics model with a maximal specific growth rate of 0.58h(-1), a half-saturation constant of 10mgl(-1), and a substrate inhibition constant of 152-550mgl(-1). The biomass yield coefficient ranged from 0.55 to 0.64mg dry cell mass/mg phenol. Due to its high tolerance to phenol and high metabolic versatility, Alcaligenes sp. TW1 is considered an excellent candidate for the biotreatment of high strength phenol-laden industrial wastewaters. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Hazardous Materials
volume
173
pages
783 - 788
publisher
Elsevier
external identifiers
  • wos:000273135600111
  • pmid:19783362
  • scopus:72149129190
ISSN
1873-3336
DOI
10.1016/j.jhazmat.2009.09.006
language
English
LU publication?
yes
id
7b8b910a-9358-4326-b306-7196c3abc95e (old id 1483133)
date added to LUP
2009-10-13 08:46:36
date last changed
2018-06-10 03:03:57
@article{7b8b910a-9358-4326-b306-7196c3abc95e,
  abstract     = {A bacterium that could completely metabolize phenol in batch culture supplied with up to 1200mg phenoll(-1) at room temperature (25 degrees C) was isolated from the activated sludge of the industrial wastewater treatment plant of a Coke company (Cairo, Egypt). Morphological and physiological characterization showed strain TW1 was a motile, strictly aerobic, gram negative and short-rod occurring singly or in clusters. Partial 16S rRNA gene sequence analysis revealed strain TW1 belonged to the beta group of Proteobacteria, showing 100% identity to Alcaligenes SCTI. Strain TW1 aerobically grew on a number of monocyclic aromatic compounds (hydroquinone, catechol and o-cresol) as well as polycyclic aromatic compounds (pyrene, phenanthrene and naphthalene). The growth of Alcaligenes TW1 on phenol as sole carbon and energy source (25 degrees C) was well described by the Haldane kinetics model with a maximal specific growth rate of 0.58h(-1), a half-saturation constant of 10mgl(-1), and a substrate inhibition constant of 152-550mgl(-1). The biomass yield coefficient ranged from 0.55 to 0.64mg dry cell mass/mg phenol. Due to its high tolerance to phenol and high metabolic versatility, Alcaligenes sp. TW1 is considered an excellent candidate for the biotreatment of high strength phenol-laden industrial wastewaters.},
  author       = {Essam, Tamer and Amin, Magdy Aly and Tayeb, Ossama El and Mattiasson, Bo and Guieysse, Benoit},
  issn         = {1873-3336},
  language     = {eng},
  pages        = {783--788},
  publisher    = {Elsevier},
  series       = {Journal of Hazardous Materials},
  title        = {Kinetics and metabolic versatility of highly tolerant phenol degrading Alcaligenes strain TW1.},
  url          = {http://dx.doi.org/10.1016/j.jhazmat.2009.09.006},
  volume       = {173},
  year         = {2010},
}