Gelatin-fibrinogen cryogel dermal matrices for wound repair: Preparation, optimisation and in vitro study.
(2010) In Biomaterials 31. p.67-76- Abstract
- Macroporous sponge-like gelatin-fibrinogen (Gl-Fg) scaffolds cross-linked with different concentrations (0.05-0.5%) of glutaraldehyde (GA) were produced using cryogelation technology, which allows for the preparation of highly porous scaffolds without compromising their mechanical properties, and is a more cost-efficient process than freeze-drying. The produced Gl-Fg-GA(X) scaffolds had a uniform interconnected open porous structure with a porosity of up to 90-92% and a pore size distribution of 10-120mum. All of the obtained cryogels were elastic and mechanically stable, except for the Gl-Fg-GA(0.05) scaffolds. Swelling kinetics and degradation rate, but not the porous structure of the cryogels, were strongly dependent on the degree of... (More)
- Macroporous sponge-like gelatin-fibrinogen (Gl-Fg) scaffolds cross-linked with different concentrations (0.05-0.5%) of glutaraldehyde (GA) were produced using cryogelation technology, which allows for the preparation of highly porous scaffolds without compromising their mechanical properties, and is a more cost-efficient process than freeze-drying. The produced Gl-Fg-GA(X) scaffolds had a uniform interconnected open porous structure with a porosity of up to 90-92% and a pore size distribution of 10-120mum. All of the obtained cryogels were elastic and mechanically stable, except for the Gl-Fg-GA(0.05) scaffolds. Swelling kinetics and degradation rate, but not the porous structure of the cryogels, were strongly dependent on the degree of cross-linking. A ten-fold increase in the degree of cross-linking resulted in an almost 80-fold decrease in the rate of degradation in a solution of protease. Cryogels were seeded with primary dermal fibroblasts and the densities observed on the surface, plus the expression levels of collagen types I and III observed 5 days post-seeding, were similar to those observed on a control dermal substitute material, Integra((R)). Fibroblast proliferation and migration within the scaffolds were relative to the GA content. Glucose consumption rate was 3-fold higher on Gl-Fg-GA(0.1) than on Gl-Fg-GA(0.5) cryogels 10 days post-seeding. An enhanced cell motility on cryogels with reducing GA crosslinking was obtained after long time culture. Particularly marked cell infiltration was seen in gels using 0.1% GA as a crosslinker. The scaffold started to disintegrate after 42 days of in vitro culturing. The described in vitro studies demonstrated good potential of Gl-Fg-GA(0.1) scaffolds as matrices for wound healing. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1483141
- author
- Dainiak, Maria B ; Allan, Iain U ; Savina, Irina N ; Cornelio, Lisa ; James, Elizabeth S ; James, Stuart L ; Mikhalovsky, Sergey V ; Jungvid, Hans and Galaev, Igor LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biomaterials
- volume
- 31
- pages
- 67 - 76
- publisher
- Elsevier
- external identifiers
-
- wos:000272364600008
- pmid:19783036
- scopus:70350340966
- pmid:19783036
- ISSN
- 1878-5905
- DOI
- 10.1016/j.biomaterials.2009.09.029
- language
- English
- LU publication?
- yes
- id
- 1e156fdc-669c-4e39-bbff-f56af627219a (old id 1483141)
- date added to LUP
- 2016-04-01 11:00:50
- date last changed
- 2022-04-04 23:26:25
@article{1e156fdc-669c-4e39-bbff-f56af627219a,
abstract = {{Macroporous sponge-like gelatin-fibrinogen (Gl-Fg) scaffolds cross-linked with different concentrations (0.05-0.5%) of glutaraldehyde (GA) were produced using cryogelation technology, which allows for the preparation of highly porous scaffolds without compromising their mechanical properties, and is a more cost-efficient process than freeze-drying. The produced Gl-Fg-GA(X) scaffolds had a uniform interconnected open porous structure with a porosity of up to 90-92% and a pore size distribution of 10-120mum. All of the obtained cryogels were elastic and mechanically stable, except for the Gl-Fg-GA(0.05) scaffolds. Swelling kinetics and degradation rate, but not the porous structure of the cryogels, were strongly dependent on the degree of cross-linking. A ten-fold increase in the degree of cross-linking resulted in an almost 80-fold decrease in the rate of degradation in a solution of protease. Cryogels were seeded with primary dermal fibroblasts and the densities observed on the surface, plus the expression levels of collagen types I and III observed 5 days post-seeding, were similar to those observed on a control dermal substitute material, Integra((R)). Fibroblast proliferation and migration within the scaffolds were relative to the GA content. Glucose consumption rate was 3-fold higher on Gl-Fg-GA(0.1) than on Gl-Fg-GA(0.5) cryogels 10 days post-seeding. An enhanced cell motility on cryogels with reducing GA crosslinking was obtained after long time culture. Particularly marked cell infiltration was seen in gels using 0.1% GA as a crosslinker. The scaffold started to disintegrate after 42 days of in vitro culturing. The described in vitro studies demonstrated good potential of Gl-Fg-GA(0.1) scaffolds as matrices for wound healing.}},
author = {{Dainiak, Maria B and Allan, Iain U and Savina, Irina N and Cornelio, Lisa and James, Elizabeth S and James, Stuart L and Mikhalovsky, Sergey V and Jungvid, Hans and Galaev, Igor}},
issn = {{1878-5905}},
language = {{eng}},
pages = {{67--76}},
publisher = {{Elsevier}},
series = {{Biomaterials}},
title = {{Gelatin-fibrinogen cryogel dermal matrices for wound repair: Preparation, optimisation and in vitro study.}},
url = {{http://dx.doi.org/10.1016/j.biomaterials.2009.09.029}},
doi = {{10.1016/j.biomaterials.2009.09.029}},
volume = {{31}},
year = {{2010}},
}