Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Fibromodulin-deficient Mice Display Impaired Collagen Fibrillogenesis in Predentin as well as Altered Dentin Mineralization and Enamel Formation.

Goldberg, Michel ; Septier, Dominique ; Oldberg, Åke LU ; Young, Marian F and Ameye, Laurent G (2006) In Journal of Histochemistry and Cytochemistry 54(5). p.525-537
Abstract
To determine the functions of fibromodulin (Fmod), a small leucine-rich keratan sulfate proteoglycan in tooth formation, we investigated the distribution of Fmod in dental tissues by immunohistochemistry and characterized the dental phenotype of 1-day-old Fmod-deficient mice using light and transmission electron microscopy. Immunohistochemistry was also used to compare the relative protein expression of dentin sialoprotein (DSP), dentin matrix protein-1 (DMP 1), bone sialoprotein (BSP), and osteopontin (OPN) between Fmod-deficient mice and wild-type mice. In normal mice and rats, Fmod immunostaining was mostly detected in the distal cell bodies of odontoblasts and in the stratum intermedium and was weaker in odontoblast processes and... (More)
To determine the functions of fibromodulin (Fmod), a small leucine-rich keratan sulfate proteoglycan in tooth formation, we investigated the distribution of Fmod in dental tissues by immunohistochemistry and characterized the dental phenotype of 1-day-old Fmod-deficient mice using light and transmission electron microscopy. Immunohistochemistry was also used to compare the relative protein expression of dentin sialoprotein (DSP), dentin matrix protein-1 (DMP 1), bone sialoprotein (BSP), and osteopontin (OPN) between Fmod-deficient mice and wild-type mice. In normal mice and rats, Fmod immunostaining was mostly detected in the distal cell bodies of odontoblasts and in the stratum intermedium and was weaker in odontoblast processes and predentin. The absence of Fmod impaired dentin mineralization, increased the diameter of the collagen fibrils throughout the whole predentin, and delayed enamel formation. Immunohistochemistry provides evidence for compensatory mechanisms in Fmod-deficient mice. Staining for DSP and OPN was decreased in molars, whereas DMP 1 and BSP were enhanced. In the incisors, labeling for DSP, DMP 1, and BSP was strongly increased in the pulp and odontoblasts, whereas OPN staining was decreased. Positive staining was also seen for DMP 1 and BSP in secretory ameloblasts. Together these studies indicate that Fmod restricts collagen fibrillogenesis in predentin while promoting dentin mineralization and the early stages of enamel formation. (Less)
Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
tooth phenotype, osteopontin, dentin, enamel, dentin sialoprotein, bone sialoprotein, dentin matrix protein 1, fibromodulin
in
Journal of Histochemistry and Cytochemistry
volume
54
issue
5
pages
525 - 537
publisher
Histochemical Society
external identifiers
  • pmid:16344330
  • wos:000237101200004
  • scopus:33646026687
ISSN
0022-1554
DOI
10.1369/jhc.5A6650.2005
language
English
LU publication?
yes
id
3cda80aa-1e7e-4a61-93fc-bba67c1a9326 (old id 148731)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16344330&dopt=Abstract
date added to LUP
2016-04-01 17:01:48
date last changed
2020-10-20 02:24:08
@article{3cda80aa-1e7e-4a61-93fc-bba67c1a9326,
  abstract     = {To determine the functions of fibromodulin (Fmod), a small leucine-rich keratan sulfate proteoglycan in tooth formation, we investigated the distribution of Fmod in dental tissues by immunohistochemistry and characterized the dental phenotype of 1-day-old Fmod-deficient mice using light and transmission electron microscopy. Immunohistochemistry was also used to compare the relative protein expression of dentin sialoprotein (DSP), dentin matrix protein-1 (DMP 1), bone sialoprotein (BSP), and osteopontin (OPN) between Fmod-deficient mice and wild-type mice. In normal mice and rats, Fmod immunostaining was mostly detected in the distal cell bodies of odontoblasts and in the stratum intermedium and was weaker in odontoblast processes and predentin. The absence of Fmod impaired dentin mineralization, increased the diameter of the collagen fibrils throughout the whole predentin, and delayed enamel formation. Immunohistochemistry provides evidence for compensatory mechanisms in Fmod-deficient mice. Staining for DSP and OPN was decreased in molars, whereas DMP 1 and BSP were enhanced. In the incisors, labeling for DSP, DMP 1, and BSP was strongly increased in the pulp and odontoblasts, whereas OPN staining was decreased. Positive staining was also seen for DMP 1 and BSP in secretory ameloblasts. Together these studies indicate that Fmod restricts collagen fibrillogenesis in predentin while promoting dentin mineralization and the early stages of enamel formation.},
  author       = {Goldberg, Michel and Septier, Dominique and Oldberg, Åke and Young, Marian F and Ameye, Laurent G},
  issn         = {0022-1554},
  language     = {eng},
  number       = {5},
  pages        = {525--537},
  publisher    = {Histochemical Society},
  series       = {Journal of Histochemistry and Cytochemistry},
  title        = {Fibromodulin-deficient Mice Display Impaired Collagen Fibrillogenesis in Predentin as well as Altered Dentin Mineralization and Enamel Formation.},
  url          = {http://dx.doi.org/10.1369/jhc.5A6650.2005},
  doi          = {10.1369/jhc.5A6650.2005},
  volume       = {54},
  year         = {2006},
}