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Pichia stipitis xylose reductase helps detoxifying lignocellulosic hydrolysate by reducing 5-hydroxymethyl-furfural (HMF)

Almeida, Joao LU ; Modig, Tobias LU ; Anja, Röder; Lidén, Gunnar LU and Gorwa-Grauslund, Marie-Francoise LU (2008) In Biotechnology for Biofuels 1.
Abstract
Background: Pichia stipitis xylose reductase (Ps-XR) has been used to design Saccharomyces

cerevisiae strains that are able to ferment xylose. One example is the industrial S. cerevisiae xyloseconsuming

strain TMB3400, which was constructed by expression of P. stipitis xylose reductase and

xylitol dehydrogenase and overexpression of endogenous xylulose kinase in the industrial S.

cerevisiae strain USM21.

Results: In this study, we demonstrate that strain TMB3400 not only converts xylose, but also

displays higher tolerance to lignocellulosic hydrolysate during anaerobic batch fermentation as well

as 3 times higher in vitro HMF and furfural reduction activity than the control... (More)
Background: Pichia stipitis xylose reductase (Ps-XR) has been used to design Saccharomyces

cerevisiae strains that are able to ferment xylose. One example is the industrial S. cerevisiae xyloseconsuming

strain TMB3400, which was constructed by expression of P. stipitis xylose reductase and

xylitol dehydrogenase and overexpression of endogenous xylulose kinase in the industrial S.

cerevisiae strain USM21.

Results: In this study, we demonstrate that strain TMB3400 not only converts xylose, but also

displays higher tolerance to lignocellulosic hydrolysate during anaerobic batch fermentation as well

as 3 times higher in vitro HMF and furfural reduction activity than the control strain USM21. Using

laboratory strains producing various levels of Ps-XR, we confirm that Ps-XR is able to reduce HMF

both in vitro and in vivo. Ps-XR overexpression increases the in vivo HMF conversion rate by

approximately 20%, thereby improving yeast tolerance towards HMF. Further purification of Ps-XR

shows that HMF is a substrate inhibitor of the enzyme.

Conclusion: We demonstrate for the first time that xylose reductase is also able to reduce the

furaldehyde compounds that are present in undetoxified lignocellulosic hydrolysates. Possible

implications of this newly characterized activity of Ps-XR on lignocellulosic hydrolysate

fermentation are discussed. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
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Biotechnology for Biofuels
volume
1
publisher
BioMed Central
external identifiers
  • WOS:000272005200001
  • Scopus:47949108963
ISSN
1754-6834
DOI
10.1186/1754-6834-1-12
language
English
LU publication?
yes
id
3fada5aa-3f40-4f0a-9f8f-32b4f93a3bac (old id 1488366)
date added to LUP
2009-10-26 12:57:02
date last changed
2017-01-15 04:33:08
@article{3fada5aa-3f40-4f0a-9f8f-32b4f93a3bac,
  abstract     = {Background: Pichia stipitis xylose reductase (Ps-XR) has been used to design Saccharomyces<br/><br>
cerevisiae strains that are able to ferment xylose. One example is the industrial S. cerevisiae xyloseconsuming<br/><br>
strain TMB3400, which was constructed by expression of P. stipitis xylose reductase and<br/><br>
xylitol dehydrogenase and overexpression of endogenous xylulose kinase in the industrial S.<br/><br>
cerevisiae strain USM21.<br/><br>
Results: In this study, we demonstrate that strain TMB3400 not only converts xylose, but also<br/><br>
displays higher tolerance to lignocellulosic hydrolysate during anaerobic batch fermentation as well<br/><br>
as 3 times higher in vitro HMF and furfural reduction activity than the control strain USM21. Using<br/><br>
laboratory strains producing various levels of Ps-XR, we confirm that Ps-XR is able to reduce HMF<br/><br>
both in vitro and in vivo. Ps-XR overexpression increases the in vivo HMF conversion rate by<br/><br>
approximately 20%, thereby improving yeast tolerance towards HMF. Further purification of Ps-XR<br/><br>
shows that HMF is a substrate inhibitor of the enzyme.<br/><br>
Conclusion: We demonstrate for the first time that xylose reductase is also able to reduce the<br/><br>
furaldehyde compounds that are present in undetoxified lignocellulosic hydrolysates. Possible<br/><br>
implications of this newly characterized activity of Ps-XR on lignocellulosic hydrolysate<br/><br>
fermentation are discussed.},
  articleno    = {12},
  author       = {Almeida, Joao and Modig, Tobias and Anja, Röder and Lidén, Gunnar and Gorwa-Grauslund, Marie-Francoise},
  issn         = {1754-6834},
  language     = {eng},
  publisher    = {BioMed Central},
  series       = {Biotechnology for Biofuels},
  title        = {Pichia stipitis xylose reductase helps detoxifying lignocellulosic hydrolysate by reducing 5-hydroxymethyl-furfural (HMF)},
  url          = {http://dx.doi.org/10.1186/1754-6834-1-12},
  volume       = {1},
  year         = {2008},
}