Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

The polyphenol quercetin strongly increases homocysteine production in a human hepatoma (Hep G2) cell line.

Hultberg, Malin LU ; Isaksson, Anders LU ; Andersson, Anders S LU and Hultberg, Björn LU (2006) In Clinical Biochemistry 39(2). p.160-163
Abstract
Objective: The metabolism of homocysteine is influenced by several dietary factors, including folate, cobalamin and possibly also the intake of polyhydroxylated phenolic compounds (polyphenols), which were shown to increase plasma homocysteine (tHcy) concentration. In order to reveal the cause of the increased plasma tHcy, we have therefore investigated the effects of a polyphenol in cell cultures from human cell lines. Design and methods: We have studied the influence of the polyphenol quercetin (Quer) on intra- and extracellular homocysteine concentrations in HeLa and hepatoma cell cultures. Results: The main finding is an increased concentration of extracellular homocysteine in the presence of Quer in hepatoma cell cultures, whereas... (More)
Objective: The metabolism of homocysteine is influenced by several dietary factors, including folate, cobalamin and possibly also the intake of polyhydroxylated phenolic compounds (polyphenols), which were shown to increase plasma homocysteine (tHcy) concentration. In order to reveal the cause of the increased plasma tHcy, we have therefore investigated the effects of a polyphenol in cell cultures from human cell lines. Design and methods: We have studied the influence of the polyphenol quercetin (Quer) on intra- and extracellular homocysteine concentrations in HeLa and hepatoma cell cultures. Results: The main finding is an increased concentration of extracellular homocysteine in the presence of Quer in hepatoma cell cultures, whereas there were no significant changes of homocysteine concentration in HeLa cell cultures. There was no effect on cellular growth, as judged by cell protein. The presence of adenosyl-dialdehyde, an inhibitor of adenosyl-homocysteine hydrolase, abolished the increased extracellular concentration of homocysteine observed in hepatoma cell cultures in the presence of Quer. Conclusion: The antioxidative agent Quer strongly increased the extracellular concentration of homocysteine in hepatoma cell cultures probably due to increased cellular methylation. In the human body, the same phenomenon might lead to increased plasma tHcy. Since elevated plasma tHcy is associated with premature vascular disease, high long-lasting dietary intake of polyphenols might be harmful. The interaction between Quer and homocysteine turnover may therefore warrant a re-evaluation of polyphenols as relatively harmless antioxidative food supplements or therapeutic antioxidative agents. (c) 2005 The Canadian Society of Clinical Chemists. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
quercetin, hepatoma cell lines, HeLa cell lines, homocysteine, methylation, polyphenol
in
Clinical Biochemistry
volume
39
issue
2
pages
160 - 163
publisher
Elsevier
external identifiers
  • pmid:16330015
  • wos:000236019400010
  • scopus:32844456945
ISSN
1873-2933
DOI
10.1016/j.clinbiochem.2005.11.002
language
English
LU publication?
yes
id
746aacc1-94df-4e17-b95a-c5d4f57a89d3 (old id 148898)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16330015&dopt=Abstract
date added to LUP
2016-04-01 12:26:44
date last changed
2021-02-17 07:58:08
@article{746aacc1-94df-4e17-b95a-c5d4f57a89d3,
  abstract     = {Objective: The metabolism of homocysteine is influenced by several dietary factors, including folate, cobalamin and possibly also the intake of polyhydroxylated phenolic compounds (polyphenols), which were shown to increase plasma homocysteine (tHcy) concentration. In order to reveal the cause of the increased plasma tHcy, we have therefore investigated the effects of a polyphenol in cell cultures from human cell lines. Design and methods: We have studied the influence of the polyphenol quercetin (Quer) on intra- and extracellular homocysteine concentrations in HeLa and hepatoma cell cultures. Results: The main finding is an increased concentration of extracellular homocysteine in the presence of Quer in hepatoma cell cultures, whereas there were no significant changes of homocysteine concentration in HeLa cell cultures. There was no effect on cellular growth, as judged by cell protein. The presence of adenosyl-dialdehyde, an inhibitor of adenosyl-homocysteine hydrolase, abolished the increased extracellular concentration of homocysteine observed in hepatoma cell cultures in the presence of Quer. Conclusion: The antioxidative agent Quer strongly increased the extracellular concentration of homocysteine in hepatoma cell cultures probably due to increased cellular methylation. In the human body, the same phenomenon might lead to increased plasma tHcy. Since elevated plasma tHcy is associated with premature vascular disease, high long-lasting dietary intake of polyphenols might be harmful. The interaction between Quer and homocysteine turnover may therefore warrant a re-evaluation of polyphenols as relatively harmless antioxidative food supplements or therapeutic antioxidative agents. (c) 2005 The Canadian Society of Clinical Chemists. All rights reserved.},
  author       = {Hultberg, Malin and Isaksson, Anders and Andersson, Anders S and Hultberg, Björn},
  issn         = {1873-2933},
  language     = {eng},
  number       = {2},
  pages        = {160--163},
  publisher    = {Elsevier},
  series       = {Clinical Biochemistry},
  title        = {The polyphenol quercetin strongly increases homocysteine production in a human hepatoma (Hep G2) cell line.},
  url          = {http://dx.doi.org/10.1016/j.clinbiochem.2005.11.002},
  doi          = {10.1016/j.clinbiochem.2005.11.002},
  volume       = {39},
  year         = {2006},
}