Advanced

Influence of Internal Capsid Pressure on Viral Infection by Phage lambda

Koester, Sarah; Evilevitch, Alex LU ; Jeembaeva, Meerim LU and Weitz, David A. (2009) In Biophysical Journal 97(6). p.1525-1529
Abstract
Ejection of the genome from the virus, phage, is the initial step in the infection of its host bacterium. In vitro, the ejection depends sensitively on internal pressure within the virus capsid; however, the in vivo effect of internal pressure on infection of bacteria is unknown. Here, we use microfluidics to monitor individual cells and determine the temporal distribution of lysis due to infection as the capsid pressure is varied. The lysis probability decreases markedly with decreased capsid pressure. Of interest, the average lysis times remain the same but the distribution is broadened as the pressure is lowered.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biophysical Journal
volume
97
issue
6
pages
1525 - 1529
publisher
Cell Press
external identifiers
  • wos:000270380800002
  • scopus:70349928561
ISSN
1542-0086
DOI
10.1016/j.bpj.2009.07.007
language
English
LU publication?
yes
id
f11b917f-c25b-46f2-8e64-0f64cc74cd41 (old id 1489796)
date added to LUP
2009-10-21 12:36:51
date last changed
2017-07-30 03:54:13
@article{f11b917f-c25b-46f2-8e64-0f64cc74cd41,
  abstract     = {Ejection of the genome from the virus, phage, is the initial step in the infection of its host bacterium. In vitro, the ejection depends sensitively on internal pressure within the virus capsid; however, the in vivo effect of internal pressure on infection of bacteria is unknown. Here, we use microfluidics to monitor individual cells and determine the temporal distribution of lysis due to infection as the capsid pressure is varied. The lysis probability decreases markedly with decreased capsid pressure. Of interest, the average lysis times remain the same but the distribution is broadened as the pressure is lowered.},
  author       = {Koester, Sarah and Evilevitch, Alex and Jeembaeva, Meerim and Weitz, David A.},
  issn         = {1542-0086},
  language     = {eng},
  number       = {6},
  pages        = {1525--1529},
  publisher    = {Cell Press},
  series       = {Biophysical Journal},
  title        = {Influence of Internal Capsid Pressure on Viral Infection by Phage lambda},
  url          = {http://dx.doi.org/10.1016/j.bpj.2009.07.007},
  volume       = {97},
  year         = {2009},
}